754 Works

JOVE Manuscript

Jessica M. Miller, Nikhil M. Mardhekar, Vasanthi Rajasekaran, Jianyi Zhang & Ramaswamy Kannappan
See enclosed

JOVE Manuscript

Jessica M. Miller, Nikhil M. Mardhekar, Vasanthi Rajasekaran, Jianyi Zhang & Ramaswamy Kannappan
See enclosed

Manuscript.pdf

Ramaswamy Kannappan, Saidulu Mattapally, Pooja A. Wagle & Jianyi Zhang
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Manuscript.pdf

Ramaswamy Kannappan, Saidulu Mattapally, Pooja A. Wagle & Jianyi Zhang
See attachement

Constructing TB Care Cascades.pdf

Ramnath Subbaraman, Ruvandhi R. Nathavitharana, Kenneth H. Mayer, Srinath Satyanarayana, Vineet K. Chadha, Nimalan Arinaminpathy & Pai Madhukar
The cascade of care is a model for evaluating patient retention across sequential stages of care required to achieve a successful treatment outcome. This approach was first used to evaluate HIV care and has since been applied to other diseases. The tuberculosis (TB) com- munity has only recently started using care cascade analyses to quantify gaps in quality of care. In this article, we describe methods for estimating gaps (patient losses) and steps (patients retained)...

Polhemus.BRC.Repeated cell transplan denervation.2019.pdf

David Polhemus, Rishi Trivedi, Thomas Sharp, Zhen Li, Traci Goodchild, Amy Scarborough, Geoffrey De Couto, Eduardo Marbán & David J. Lefer
Enthusiasm for cell therapy for myocardial injury has waned due to equivocal benefits in clinical trials. In an attempt to improve efficacy, we investigated repeated cell therapy and adjunct renal denervation (RDN) as strategies for augmenting cardioprotection with cardiosphere-derived cells (CDCs). We hypothesized that combining CDC post-conditioning with repeated CDC doses or delayed RDN therapy would result in superior function and remodeling. Wistar–Kyoto (WKY) rats or spontaneously hypertensive rats (SHR) were subjected to 45 min...

Pica in a Four-Year-Old Girl with Global Developmental Delay_2015.pdf

Yi-Hui Liu, Megan Pesch, Julie Lumeng & Martina Stein
CASE: Emily is a 4 and half-year-old girl whose foster mother is concerned about her odd eating behaviors.Emily has been with her foster mother for 1 year after exposure to domestic violence. Emily’s habit of eatingnonfood items led to her foster mother providing “100% supervision.” Emily constantly picks up, smells, andtastes nonfood items, particularly rocks and things made of metal. She “explores everything with hertongue.” Emily scoops dirt and gravel from sidewalk crevices into her...

The promise and peril of universal health care

David E. Bloom, Alexander Khoury & Ramnath Subbaraman
Universal health care (UHC) is garnering growing support throughout the world, a reflection of social and economic progress and of the recognition that population health is both an indicator and an instrument of national development. Substantial human and financial resources will be required to achieve UHC in any of the various ways it has been conceived and defined. Progress toward achieving UHC will be aided by new technologies, a willingness to shift medical tasks from...

Influence of oral progesterone on drug-induced QT interval lengthening, JACC Clin EP 2016.pdf

Tisdale JE, Jaynes HA, Overholser BR, Sowinski KM, Flockhart DA & Kovacs RJ
Published journal article describing the results of this work

Nat Commun-De novo adipocyte differentiation from Pdgfrβ+ preadipocytes protects against pathologic visceral adipose expansion in obesity.pdf

Mengle Shao, Lavanya Vishvanath, Napoleon Busbuso, Chelsea Hepler, Bo Shan, Ankit Sharma, Shiuhwei Chen, Xinxin Yu, Yu An, Yi Zhu, William Holland & Rana Gupta
Pathologic expansion of white adipose tissue (WAT) in obesity is characterized by adipocytehypertrophy, inflammation, and fibrosis; however, factors triggering this maladaptive remodelingare largely unknown. Here, we test the hypothesis that the potential to recruit newadipocytes from Pdgfrβ+ preadipocytes determines visceral WAT health in obesity. Wemanipulate levels of Pparg, the master regulator of adipogenesis, in Pdgfrβ+ precursors ofadult mice. Increasing the adipogenic capacity of Pdgfrβ+ precursors through Pparg overexpressionresults in healthy visceral WAT expansion in obesity...

Insulin regulates titin pre-mRNA splicing through the PI3K-Akt-mTOR kinase axis in a RBM20-dependent manner

Chaoqun Zhu
Titin, a giant sarcomeric protein, is largely responsible for the diastolic properties of the heart. It has two major isoforms, N2B and N2BA due to pre-mRNA splicing regulated mainly by a splicing factor RNA binding motif 20 (RBM20). Mis-splicing of titin pre-mRNA in response to external stimuli may lead to altered ratio of N2B to N2BA, and thus, impaired cardiac contractile function. However, little is known about titin alternative splicing in response to external stimuli....

Reactive oxygen species damage drives cardiac and mitchondrial dysfunction following acute nano titanium dioxide inhalation exposure.pdf

Cody E. Nichols, Danielle L. Shepherd, Quincy A. Hathaway, Andrya J. Durr, Dharendra Thapa, Alaeddin Abukabda, Jinghai Yi, Timothy R. Nurkiewicz & John M. Hollander
The goal of this study was to determine the extent of ROS involvement on cardiac function and the mitochondrion following nano-TiO2 exposure.

IRE1α inhibition decreased TXNIP/NLRP3 inflammasome activation through miR-17-5p after neonatal hypoxic–ischemic brain injury in rats

Jiping Tang
IRE1a-induced UPR pathway may contribute to inflammatory activation and brain injury following neonatal HI. IRE1a activation, through decay of miR-17-5p, elevated TXNIP expression to activate NLRP3 inflammasomeand aggravated brain damage.

Transplantation of Cardiac Mesenchymal Stem Cell-Derived Exosomes Promotes Repair in Ischemic Myocardium

Yaoliang Tang
intramyocardial delivery of C-MSC-Exo after myocardial infarction enhances cardiac angiogenesis, promotes cardiomyocyte proliferation, and preserves heart function. C-MSC-Exo constitute a novel form of cell-freetherapy for cardiac repair

Mothers of obese children use more direct imperatives to restrict eating_2017.pdf

Megan Pesch, Allison L. Miller, Danielle Appugliese, Katherine L. Rosenblum & Julie Lumeng
ABSTRACTObjective: To examine the association of mother and child characteristics with use of direct imperativesto restrict eating.Methods: A total of 237 mother–child dyads (mean child age, 70.9 months) participated in a videorecorded,laboratory-standardized eating protocol with 2 large portions of cupcakes. Videos were reliablycoded for counts of maternal direct imperatives to restrict children’s eating. Anthropometrics were measured.Regression models tested the association of participant characteristics with counts of direct imperatives.Results: Child obese weight status and maternal white...

KLF11 (Krüppel-Like Factor 11) Inhibits Arterial Thrombosis via Suppression of Tissue Factor in the Vascular Wall

Wenying Liang, Yanbo Fan, Haocheng Lu, Jinjian Sun, Ziyi Chang, Wenting Hu, Huilun Wang, Tianqing Zhu, Jintao Wang, Reheman Adili, Minerva Garcia-Barrio, Michael Holinstat, Daniel T. Eitzman, Jifeng Zhang & Y. Eugene Chen
Using a ferric chloride–induced thrombosis model, we found that the occlusion time was significantly reduced in conventional Klf11 knockout mice, whereas bone marrow transplantation could not rescue this phenotype, suggesting that vascular KLF11 is critical for inhibition of arterial thrombosis. We further demonstrated that vascular smooth muscle cell–specific Klf11 knockout mice also exhibited significantly reduced occlusion time. The expression of tissue factor (encoded by the F3 gene), a main initiator of the coagulation cascade, was...

Forrester et al., 2018.pdf

Steven J. Forrester, Daniel Kikuchi, Marina S. Hernandes, Qian Xu & Kathy K. Griendling
Reactive oxygen species (ROS) are well known for their role in mediating both physiological and pathophysiological signal transduction. Enzymes and subcellular compartments that typically produce ROS are associated with metabolic regulation, and diseases associated with metabolic dysfunction may be influenced by changes in redox balance. In this review, we summarize the current literature surrounding ROS and their role in metabolic and inflammatory regulation, focusing on ROS signal transduction and its relationship to disease progression. In...

PIP2 depletion promotes TRPV4 channel activity in mouse brain capillary endothelial cells

Osama F. Harraz, Thomas A. Longden, David Hill-Eubanks & Mark T Nelson
We recently reported that the inward-rectifier Kir2.1 channel in brain capillary endothelial cells (cECs) plays a major role in neurovascular coupling (NVC) by mediating a neuronal activity-dependent, propagating vasodilatory (hyperpolarizing) signal. We further demonstrated that Kir2.1 activity is suppressed by depletion of plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP2). Whether cECs express depolarizing channels that intersect with Kir2.1-mediated signaling remains unknown. Here, we report that Ca2+/Na+-permeable TRPV4 (transient receptor potential vanilloid 4) channels are expressed in cECs...

Carnosine modulates nitric oxide in stimulated murine RAW 264.7 macrophages

Giuseppe Caruso, Claudia G. Fresta, Francisco J. Martinez-Becerra, Antonio Lopalco, Ryan T. Johnson, Richard P. S. De Campos, Joseph M. Siegel, Manjula B. Wijesinghe, Giuseppe Lazzarino & Susan M. Lunte
Excess nitric oxide (NO) production occurs in several pathological states, including neurodegeneration, ischemia, and inflammation. Carnosine has been reported to decrease oxidative/nitrosative stress-associated cell damage by reducing the amount of NO produced. In this study, we evaluated the effect of carnosine on NO production by macrophages stimulated with LPS + IFN-γ. Intracellular NO and intracellular and extracellular nitrite were measured by microchip electrophoresis with laser-induced fluorescence and by the Griess assay, respectively. Results showed that...

Vincentz et al., 2016, PNAS.pdf

Joshua Vincentz, Jianwen Que & Anthony B. Firulli
Cranial neural crest cells (crNCCs) migrate from the neural tube to the pharyngeal arches (PAs) of the developing embryo and, sub- sequently, differentiate into bone and connective tissue to form the mandible. Within the PAs, crNCCs respond to local signaling cues to partition into the proximo-distally oriented subdomains that convey positional information to these developing tissues. Here, we show that the distal-most of these subdomains, the distal cap, is marked by expression of the transcription...

Engineering approaches to prevent blood clotting from medical implants

Aaron Wilson, Pierre Neuenschwander & Shih-Feng Chou
Injectable and/or Implantable medical devices are widely used in the treatment of diseases. Among them, vascular stents provide the medical solution to treat blood clotting. However, traditional metallic stents, even with current improvements in anticoagulation properties, have potential drawbacks in local inflammation when first implanted into the body and undesirable protein adsorption and cell adhesion after a prolonged period of time in the body. In this perspective, we discuss several engineering approaches, including drug-eluting materials,...

Gizaw et al. - 2019 - The role of electrospun fiber scaffolds in stem ce.pdf

Mulugeta Gizaw, Addison Faglie, Martha Pieper, Sarju Poudel & Shih-Feng Chou
Stem cell therapy has emerged as one of the topics in tissue engineering where undifferentiated and multipotent cells are strategically placed/ injected in tissue structure for cell regeneration. Over the years, stem cells have shown promising results in skin repairs for non-healing and/or chronic wounds. The addition of the stem cells around the wound site promotes signaling pathways for growth factors that regulate tissue reconstruction. However, injecting stem cells around the wound site has its...

Nox2 contributes to hyperinsulinemia-induced redox imbalance and impaired vascular function.pdf

Abeer M Mahmoud, Mohamed M Ali, Edwin R. Miranda, Jacob T. Mey, Brian K. Blackburn, Jacob M. Haus & Shane A. Phillips
Hyperinsulinemia impaired vasodilation and nitric oxide bioavailability via inducing NADPH oxidase activity and oxidative stress

Beta2AR-Dependent Chemokine Receptor 2 Expression Regulates Leukocyte Recruitment to teh Heart Following Acute Injury.pdf

Laurel A. Grisanti, Christopher J. Traynham, Ashley A. Repas, Erhe Gao, Walter J. Koch & Douglas G. Tilley
Following cardiac injury, early immune cell responses are essential
for initiating cardiac remodeling and tissue repair. We previously
demonstrated the importance of β2-adrenergic receptors (β2ARs)
in the regulation of immune cell localization following acute cardiac
injury, with deficient leukocyte infiltration into the damaged
heart. The purpose of this study was to investigate the mechanism
by which immune cell-expressed β2ARs regulate leukocyte recruitment
to the heart following acute cardiac injury. Chemokine receptor
2 (CCR2) expression and responsiveness to C-C motif chemokine
ligand 2 (CCL2)-mediated migration...

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