31 Works

Woman skeletal muscle transcriptome with bed rest and countermeasures

Angèle Chopard, Martine Lecunff, Richard Danger, Guillaume Lamirault, Audrey Bihouee, Raluca Teusan, Bernard Jasmin, Jean-François Marini & Jean Leger
Microgravity has a dramatic impact on human physiology, illustrated in particular with skeletal muscle impairment. A thorough understanding of the mechanisms leading to loss of muscle mass and structural disorders is necessary for the definition of efficient clinical and spaceflight countermeasures. We investigated the effects of long-term bed rest on transcriptome of soleus (SOL) and vastus lateralis (VL) muscles in healthy women (BRC group, n=8), and the potential beneficial impact of protein supplementation (BRN group,...

Women International Space Simulation for Exploration (WISE)

Bernard Jasmin & Angèle Chopard
Based in Toulouse (France), Institute for Space Medicine and Physiology develop an expertise in the medical supervision of manned flights and the preparations for future interplanetary manned missions. It undertakes experiments which simulate the effects of the Space environment (involving bed rest, confinement, circadian rhythms, etc) in order to study its physiological effects. Space exploration and interplanetary travel requires that humans be confronted with extended durations of space environment and weightlessness. From a few days...

Evaluation of MALDI-TOF Mass spectrometry detection of DNA noncovalent complexes with basic polypeptides or polyamines

Peran Terrier, Jeanine Tortajada, Gregoire Zin & Wiliam Buchmann
In this work, MALDI-MS was evaluated as a method for the study of noncovalent complexes involving DNA and basic polypeptides (penta-L-arginine, penta-L-lysine) or polyamines (polypropylenimine dendrimers, linear and branched polyethylenimines).

Analysis of Synthetic Polymers by LC-MS using an APCI Interface

Bernard Desmazières, Wiliam Buchmann, Peran Terrier & Jeanine Tortajada
In this work, the performance of the APCI interface will be investigated in SEC and LC-MS coupling applied to the analysis of industrial polymers up to molecular masses of 5 kDa: polyethers; polysiloxanes, and copolymers of siloxanes. Representative applications will be described to highlight benefits and limitations of the APCI technique.

Mass Spectrometry methods for the analysis of synthetic polymers and DNA noncovalent complexes

Wiliam Buchmann
In this work, the performance of mass spectrometry will be investigated for the analysis of industrial polymers (polyethers; polysiloxanes, and copolymers of siloxanes) and noncovalent complexes( DNA and polypeptides or polyamines).

Effect of sulfated oligosaccharides (heparin and fucoidan) on the oligomerization of stromal cell-derived factor-1

Soraya Fermas, Florence Gonnet, Angela Sutton, Nathalie Charnaux, Barbara Mulloy, Yuguo Du, Françoise Baleux & Régis Daniel
We investigate the dimerization of SDF-1 in microM concentration and in the presence of sulfated oligosaccharides, using affinity capillary electrophoresis (ACE) hyphenated to electrospray ionization-mass spectrometry (ESI/MS). Chemokine stromal cell-derived factor-1 (SDF-1) is a potent chemoattractant involved in leukocyte trafficking and metastasis. Heparan sulfate on the cell surface binds SDF-1 and may modulate its function as a coreceptor of this chemokine. A major effect of the glycosaminoglycan binding may be on the quaternary structure of...

Analysis of sulfated oligosaccharrides by Mass Spectrometry

Florence Gonnet, Régis Daniel & Jeanine Tortajada
The structural heterogeneity of fucoidan with respect to linkage and sulfate position is still a limitation to trace clear correlations between the structure and biological activities of this polysaccharide, although much effort has been made during the past years to elucidate its structure complexes( DNA and polypeptides or polyamines). Recent studies have demonstrated the usefulness of tandem mass spectrometry for the sequencing of glycosaminoglycan oligosaccharides and the differentiation of sulfated isomers. Indeed, the fragmentation of...

Response of Caenorhabditis elegans to infection by Drechmeria coniospora at 24 hours post exposure

Jonathan Ewbank
Transcription profiling of Caenorhabditis elegans after an exposure of 4 hours to the fungal pathogen Drechmeria coniospora, and a post exposure of 24 hours. This experiment has been deposited in the Array Express database under reference number E-MEXP-768.

Response of Caenorhabditis elegans to infection by Drechmeria coniospora at 12 hours post exposure

Jonathan Ewbank
Transcription profiling of Caenorhabditis elegans after an exposure of 4 hours to the fungal pathogen Drechmeria coniospora, and a post exposure of 12 hours. This experiment has been deposited in the Array Express database under reference number E-MEXP-767.

Transcription profiling of Caenorhabditis elegans 12 hours and 24 hours post exposure to fungal pathogen, Drechmeria coniospora

Jonathan Ewbank
Transcriptome study of Caenorhabditis elegans animals 12 hours and 24 hours post exposure to fungal pathogen, Drechmeria coniospora. This experiment has been deposited in the Array Express database under reference number E-MEXP-479.

Response of Caenorhabditis elegans to infection by Drechmeria coniospora

Jonathan Ewbank
Caenorhabditis elegans is a widely used invertebrate model organism. We are using C. elegans to dissect the molecular basis of host-pathogen interactions. We are investigating host innate immune defences. There is mounting evidence that resistance to infection in the nematode involves both a dedicated antimicrobial response and a more general reaction to infection-associated cellular stress. Our studies indicate that multiple signal pathways cooperate to control the expression of antimicrobial peptides and proteins. We are starting...

NMR characterization of I19L peptide interacting with FtsZ

Marie-Jeanne Clément, Kuoch Boi-Trinh, Ha-Duong Tap, Joshi Vandana, Loic Hamon, Flavio Toma, Patrick Curmi & Philippe Savarin
FtsZ is a prokaryotic tubulin-like protein. Despite a low degree of sequence identity with tubulin, it presents the same folding pattern and some similar functions, notably in cell division. Indeed, FtsZ and tubulin polymerize to form bundles and microtubules, respectively, which are essential for cell cytokinesis. STD-NMR and TRNOESY experiments revealed that I19L interacts with FtsZ and folds upon its binding but in a way different from what we observed with tubulin. These NMR data...

NMR characterization of I19L peptide interacting with tubulin

Marie-Jeanne Clement, Isabelle Jourdain, Sylvie Lachkar, Philippe Savarin, Benoit Gigant, Marcel Knossow, Flavio Toma, Andre Sobel & Patrick Curmi
In the present study, we demonstrate that such peptides derived from stathmin family proteins function as potent autonomous inhibitors of tubulin assembly. The NMR structural study of I19L, the most efficient of these peptides, revealed that, while the free peptide has no preferred conformation, it adopts in solution a beta-hairpin structure upon interaction with tubulin, close to that observed in tubulin-SLD crystals by X-ray analysis. Saturation transfer difference NMR (STD-NMR) spectroscopy allowed us to identify...

Structure analysis of a peptide interacting with tubulin or FtsZ

Philippe Savarin & Patrick Curmi
Understanding the cytoskeleton is required for understanding the dynamic organisation of the intracellular space. In eukaryotic cells, microtubules are one of the major components. It has been demonstrated in the present study that a peptide extracted from the N-terminal of Stathmin-like domain interacts with tubulin and impedes microtubule assembly. Interestingly, it has been demonstrated that one of this peptide was able to interact with FtsZ, the prokaryotic homologue of the tubulin.

MicroRNA expression pattern in primary lung cancers.

Marie-Pierre Puissegur, Pascal Barbry, Kevin Lebrigand, Véronique Hofman, Paul Hofman & Bernard Mari
MicroRNA expression pattern was analysed in 20 pairs of primary lung cancers and their corresponding non cancerous lung tissues. These specimens were obtained from the Nice Hospital Tumor Bio Bank, France. Dye swap-experiment were designed by comparing cancerous tissue versus adjacent normal lung tissue.This experiment has been deposited in the Gene Expression Omnibus (GEO) database under reference number GSE18692.

Gene expression analysis in primary lung cancers.

Marie-Pierre Puissegur, Pascal Barbry, Kevin Lebrigand, Véronique Hofman, Paul Hofman & Bernard Mari
Gene expression was analysed in 13 pairs of primary lung cancers and their corresponding non cancerous lung tissues. The specimens were obtained from the Nice Hospital Tumor Bio Bank, France. Dye swap-experiment were designed by comparing cancerous tissue versus adjacent normal lung tissue.This experiment has been deposited in the Gene Expression Omnibus (GEO) database under reference number GSE18694.

Identification of putative novel specific targets of mir-210 in A549 human adenocarcinoma cells

Bernard Mari, Marie-Pierre Puissegur, Pascal Barbry & Kevin Lebrigand
To identify putative novel specific targets of mir-210, we overexpressed miR-210 as well as miR-34a and a siRNA targeted against E2F3 in A549 human adenocarcinoma cells by transfecting them with synthetic pre-miRNAs or a synthetic negative pre-miRNA as control (miR-Neg). RNA samples were harvested at 48 hours post-transfection and 2 independent experiments performed in dye-swap: miR-210 versus miR-Neg ; miR-34a versus miR-Neg ; si-E2F3 versus miR-Neg ; si-control versus miR-Neg.This experiment has been deposited in...

MiRNA and lung cancer (INCa project)

Pascal Barbry & Bernard Mari
Cites: Ortholan C, Puissegur MP, Ilie M, Barbry P, Mari B, Hofman P. MicroRNAs and lung cancer: new oncogenes and tumor suppressors, new prognostic Factors and Potential Therapeutic Targets. Curr Med Chem. 2009;16(9):1047-61. Review. PMID: 19275611.

Transcription profiling of Ikaros deficient plasmacytoid dendritic cells

Philippe Kastner
Ikaros is an important regulator of early dendritic cell development. To determine whether bone marrow plasmacytoid dendritic cells are affected by Ikaros decifiency, the transcription profilings of these cells has been compared to those of wild-type plasmacytoid cells.

Role of Ikaros in hematopoietic cell development

Philippe Kastner
The hematopoietic cell-specific transcription factor Ikaros functions as a key regulator of lymphocyte differentiation and a tumor suppressor in T cells. The Ikaros gene encodes a zinc-finger transcription factor required during early lymphocyte development. We study Ikaros function in lymphocyte cell lineage using mice bearing a hypomorphic mutation in the ikzf1 (Ikaros) locus (IkL/L).

Comparison of mouse NK cell subsets defined by CD27 and CD11b expression

Thierry Walzer & Eric Vivier
Previous reports have defined three subsets of mouse NK cells on the basis of the expression of CD27 and CD11b. The developmental relationship between these subsets was unclear. To address this issue, we evaluated the overall proximity between mouse NK cell subsets defined by CD27 and CD11b expression using pangenomic gene expression profiling. The results suggest that CD27+CD11b-, CD27+CD11b+ and CD27-CD11b+ correspond to three different intermediates stages of NK cell development. This experiment has been...

Differential roles of ROR gamma-t in the development of NKp46+ spleen (LTIL) cells and NKp46+ NK cells in gut and skin

Damien Chaussabel, Elena Tomasello, Eric Vivier, Carmelo Luci, Ana Reynders & Ivaylo Ivanov
Natural killer (NK) cells are NKp46+CD3- lymphocytes that can perform granule-dependent cytotoxicity and produce interferon-gamma, when isolated from blood, lymphoid organs, lung, liver and uterus. Here we identify in dermis, gut lamina propria and cryptopatches, very distinct populations of NKp46+CD3- cells with reduced ability to degranulate and to produce interferon-gamma. In gut, the transcription factor RORgamma-t and CD127 (IL-7R alpha) defined a novel subset of NKp46+CD3- that is reminiscent of lymphoid tissue inducer (LTi)-like cells....

Natural killer cells and innate immunity

Eric Vivier
Natural Killer (NK) cells are lymphocytes of the innate immune system that recognize and induce the lysis of a variety of target cells, including virally-infected cells, tumor cells, and allogenic cells without prior specific sensitization. In addition, NK cells elaborate a variety of regulatory cytokines including IFNg, TGFß1, TNFa, IL-1bß, IL-10, G-CSF, and GM-CSF and CC-chemokines, such as RANTES, MIP-1a and MIP-1bß which are involved in the elimination of intracellular pathogens in vivo, as well...

Deciphering Transcriptional Regulatory Networks that Control Differentiation, Identity and Proliferation of Murine ES and T Cells

Pierre Ferrier & Jean-Christophe Andrau
We are interested in transcription factor binding patterns both in murine ES cells and in differentiated T cells. Among others we study genome wide recruitment of RNA poymerase II, TBP, polycomb and other TFs in these cells using either chip on chip or ChIPseq approaches.

Suz12 ChIP-on-chip of mouse embryonic stem cell

Pierre Cauchy, Touati Benoukraf, Romain Fenouil, Frédéric Koch, Jean-Christophe Andrau & Pierre Ferrier
We aim to identify target sequences of suz12, a member of the polycomb group proteins (PcG) PRC2 (polycomb repressive complex 2). We study the role played by this polycomb complex on genetic plasticity during embryonic development in undifferentiated mouse embryonic stem cells (day 8). Suz12 is required for trimethylation of the tail of histone H3 on lysine 27 (H3K27me3). This epigenetic modification of chromatin induces dynamic repression of many target genes involved in developmental pathways....

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Publication Year

  • 2010
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Registration Year

  • 2010
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