22 Works

Response of Caenorhabditis elegans to infection by Drechmeria coniospora at 12 hours post exposure

Jonathan Ewbank
Transcription profiling of Caenorhabditis elegans after an exposure of 4 hours to the fungal pathogen Drechmeria coniospora, and a post exposure of 12 hours. This experiment has been deposited in the Array Express database under reference number E-MEXP-767.

Comparison of mouse dendritic cell subsets by genome-wide expression profiling

Marc Dalod, Robbins Scott, Doulaye Dembele, Philippe Kastner & Susan Chan
Spleen or lymph node resident DCs are subdivided into conventional DC (cDC) subsets (CD11b and CD8alpha in mouse; BDCA1 and BDCA3 in man) and plasmacytoid DCs (pDCs). It is currently unclear if these various DC populations belong to a unique hematopoietic lineage and if the subsets identified in the mouse and human systems are evolutionary homologs. To bring novel insights into these questions, we sought conserved genetic signatures for these DCs through the analysis of...

NMR characterization of I19L peptide interacting with FtsZ

Marie-Jeanne Clément, Kuoch Boi-Trinh, Ha-Duong Tap, Joshi Vandana, Loic Hamon, Flavio Toma, Patrick Curmi & Philippe Savarin
FtsZ is a prokaryotic tubulin-like protein. Despite a low degree of sequence identity with tubulin, it presents the same folding pattern and some similar functions, notably in cell division. Indeed, FtsZ and tubulin polymerize to form bundles and microtubules, respectively, which are essential for cell cytokinesis. STD-NMR and TRNOESY experiments revealed that I19L interacts with FtsZ and folds upon its binding but in a way different from what we observed with tubulin. These NMR data...

Transcription profiling of Caenorhabditis elegans 12 hours and 24 hours post exposure to fungal pathogen, Drechmeria coniospora

Jonathan Ewbank
Transcriptome study of Caenorhabditis elegans animals 12 hours and 24 hours post exposure to fungal pathogen, Drechmeria coniospora. This experiment has been deposited in the Array Express database under reference number E-MEXP-479.

Analysis of sulfated oligosaccharrides by Mass Spectrometry

Florence Gonnet, Régis Daniel & Jeanine Tortajada
The structural heterogeneity of fucoidan with respect to linkage and sulfate position is still a limitation to trace clear correlations between the structure and biological activities of this polysaccharide, although much effort has been made during the past years to elucidate its structure complexes( DNA and polypeptides or polyamines). Recent studies have demonstrated the usefulness of tandem mass spectrometry for the sequencing of glycosaminoglycan oligosaccharides and the differentiation of sulfated isomers. Indeed, the fragmentation of...

Suz12 ChIP-on-chip of mouse embryonic stem cell

Pierre Cauchy, Touati Benoukraf, Romain Fenouil, Frédéric Koch, Jean-Christophe Andrau & Pierre Ferrier
We aim to identify target sequences of suz12, a member of the polycomb group proteins (PcG) PRC2 (polycomb repressive complex 2). We study the role played by this polycomb complex on genetic plasticity during embryonic development in undifferentiated mouse embryonic stem cells (day 8). Suz12 is required for trimethylation of the tail of histone H3 on lysine 27 (H3K27me3). This epigenetic modification of chromatin induces dynamic repression of many target genes involved in developmental pathways....

Major metabolites of Charentais melon flesh

Benoit Biais, J William Allwood, Catherine Deborde, Yun Xu, Mickael Maucourt, Bertrand Beauvoit, Warwick B Dunn, Daniel Jacob, Royston Goodacre, Dominique Rolin & Annick Moing
The major metabolites of melon flesh of three F1 hybrid cultivars (Cézanne, Escrito and Hugo) of Charentais melon (Cucumis melo L.) were analysed by quantitative 1H-NMR of polar extracts. This experiment has been deposited in the MeRy-B database, a plant metabolomics platform allowing the storage and visualisation of Nuclear Magnetic Resonance (NMR) metabolic profiles from plants (http://www.cbib.u-bordeaux2.fr/MERYB/).

Effect of sulfated oligosaccharides (heparin and fucoidan) on the oligomerization of stromal cell-derived factor-1

Soraya Fermas, Florence Gonnet, Angela Sutton, Nathalie Charnaux, Barbara Mulloy, Yuguo Du, Françoise Baleux & Régis Daniel
We investigate the dimerization of SDF-1 in microM concentration and in the presence of sulfated oligosaccharides, using affinity capillary electrophoresis (ACE) hyphenated to electrospray ionization-mass spectrometry (ESI/MS). Chemokine stromal cell-derived factor-1 (SDF-1) is a potent chemoattractant involved in leukocyte trafficking and metastasis. Heparan sulfate on the cell surface binds SDF-1 and may modulate its function as a coreceptor of this chemokine. A major effect of the glycosaminoglycan binding may be on the quaternary structure of...

Natural killer cells and innate immunity

Eric Vivier
Natural Killer (NK) cells are lymphocytes of the innate immune system that recognize and induce the lysis of a variety of target cells, including virally-infected cells, tumor cells, and allogenic cells without prior specific sensitization. In addition, NK cells elaborate a variety of regulatory cytokines including IFNg, TGFß1, TNFa, IL-1bß, IL-10, G-CSF, and GM-CSF and CC-chemokines, such as RANTES, MIP-1a and MIP-1bß which are involved in the elimination of intracellular pathogens in vivo, as well...

Deciphering Transcriptional Regulatory Networks that Control Differentiation, Identity and Proliferation of Murine ES and T Cells

Pierre Ferrier & Jean-Christophe Andrau
We are interested in transcription factor binding patterns both in murine ES cells and in differentiated T cells. Among others we study genome wide recruitment of RNA poymerase II, TBP, polycomb and other TFs in these cells using either chip on chip or ChIPseq approaches.

Metabolomics of Charentais Melon Fruits

Catherine Deborde
Metabolomics approaches using 1H-NMR and GC-EI-TOF-MS profiling were employed to characterise three F1 hybrid cultivars (Cézanne, Escrito and Hugo) of Charentais melon (Cucumis melo L.). This experiment has been deposited in the MeRy-B database, a plant metabolomics platform allowing the storage and visualisation of Nuclear Magnetic Resonance (NMR) metabolic profiles from plants (http://www.cbib.ubordeaux2.fr/MERYB/).

Response of Caenorhabditis elegans to infection by Drechmeria coniospora

Jonathan Ewbank
Caenorhabditis elegans is a widely used invertebrate model organism. We are using C. elegans to dissect the molecular basis of host-pathogen interactions. We are investigating host innate immune defences. There is mounting evidence that resistance to infection in the nematode involves both a dedicated antimicrobial response and a more general reaction to infection-associated cellular stress. Our studies indicate that multiple signal pathways cooperate to control the expression of antimicrobial peptides and proteins. We are starting...

Role of Ikaros in hematopoietic cell development

Philippe Kastner
The hematopoietic cell-specific transcription factor Ikaros functions as a key regulator of lymphocyte differentiation and a tumor suppressor in T cells. The Ikaros gene encodes a zinc-finger transcription factor required during early lymphocyte development. We study Ikaros function in lymphocyte cell lineage using mice bearing a hypomorphic mutation in the ikzf1 (Ikaros) locus (IkL/L).

Spatial localisation of metabolites in the Charentais melon fruit

Benoit Biais, J William Allwood, Catherine Deborde, Yun Xu, Mickael Maucourt, Bertrand Beauvoit, Warwick B Dunn, Daniel Jacob, Royston Goodacre, Dominique Rolin & Annick Moing
Spatial localisation of metabolites was investigated on one representative fruit for each cultivar by direct 1H-NMR profiling of juice or GC-EI-TOF-MS profiling of tissue extracts collected from different locations in the fruit flesh. This experiment has been deposited in the MeRy-B database, a plant metabolomics platform allowing the storage and visualisation of Nuclear Magnetic Resonance (NMR) metabolic profiles from plants (http://www.cbib.u-bordeaux2.fr/MERYB/).

Gene expression analysis in primary lung cancers.

Marie-Pierre Puissegur, Pascal Barbry, Kevin Lebrigand, Véronique Hofman, Paul Hofman & Bernard Mari
Gene expression was analysed in 13 pairs of primary lung cancers and their corresponding non cancerous lung tissues. The specimens were obtained from the Nice Hospital Tumor Bio Bank, France. Dye swap-experiment were designed by comparing cancerous tissue versus adjacent normal lung tissue.This experiment has been deposited in the Gene Expression Omnibus (GEO) database under reference number GSE18694.

Analysis of Synthetic Polymers by LC-MS using an APCI Interface

Bernard Desmazières, Wiliam Buchmann, Peran Terrier & Jeanine Tortajada
In this work, the performance of the APCI interface will be investigated in SEC and LC-MS coupling applied to the analysis of industrial polymers up to molecular masses of 5 kDa: polyethers; polysiloxanes, and copolymers of siloxanes. Representative applications will be described to highlight benefits and limitations of the APCI technique.

Differential roles of ROR gamma-t in the development of NKp46+ spleen (LTIL) cells and NKp46+ NK cells in gut and skin

Damien Chaussabel, Elena Tomasello, Eric Vivier, Carmelo Luci, Ana Reynders & Ivaylo Ivanov
Natural killer (NK) cells are NKp46+CD3- lymphocytes that can perform granule-dependent cytotoxicity and produce interferon-gamma, when isolated from blood, lymphoid organs, lung, liver and uterus. Here we identify in dermis, gut lamina propria and cryptopatches, very distinct populations of NKp46+CD3- cells with reduced ability to degranulate and to produce interferon-gamma. In gut, the transcription factor RORgamma-t and CD127 (IL-7R alpha) defined a novel subset of NKp46+CD3- that is reminiscent of lymphoid tissue inducer (LTi)-like cells....

Comparison of mouse NK cell subsets defined by CD27 and CD11b expression

Thierry Walzer & Eric Vivier
Previous reports have defined three subsets of mouse NK cells on the basis of the expression of CD27 and CD11b. The developmental relationship between these subsets was unclear. To address this issue, we evaluated the overall proximity between mouse NK cell subsets defined by CD27 and CD11b expression using pangenomic gene expression profiling. The results suggest that CD27+CD11b-, CD27+CD11b+ and CD27-CD11b+ correspond to three different intermediates stages of NK cell development. This experiment has been...

Dendritic cells and antiviral defenses

Marc Dalod
The goal of our research is to identify new molecular pathways regulating the biology of DC subsets or more generally innate immune responses to viral infection. The model used is murine cytomegalovirus (MCMV) infection. The specific aims are 1) to determine the molecular bases regulating in vivo differentiation and anti-viral functions of DC subsets, 2) to investigate further NK cell anti-viral and immunoregulatory functions during viral infection, and 3) to identify viral molecules and cellular...

Response of Caenorhabditis elegans to infection by Drechmeria coniospora at 24 hours post exposure

Jonathan Ewbank
Transcription profiling of Caenorhabditis elegans after an exposure of 4 hours to the fungal pathogen Drechmeria coniospora, and a post exposure of 24 hours. This experiment has been deposited in the Array Express database under reference number E-MEXP-768.

Woman skeletal muscle transcriptome with bed rest and countermeasures

Angèle Chopard, Martine Lecunff, Richard Danger, Guillaume Lamirault, Audrey Bihouee, Raluca Teusan, Bernard Jasmin, Jean-François Marini & Jean Leger
Microgravity has a dramatic impact on human physiology, illustrated in particular with skeletal muscle impairment. A thorough understanding of the mechanisms leading to loss of muscle mass and structural disorders is necessary for the definition of efficient clinical and spaceflight countermeasures. We investigated the effects of long-term bed rest on transcriptome of soleus (SOL) and vastus lateralis (VL) muscles in healthy women (BRC group, n=8), and the potential beneficial impact of protein supplementation (BRN group,...

Evaluation of MALDI-TOF Mass spectrometry detection of DNA noncovalent complexes with basic polypeptides or polyamines

Peran Terrier, Jeanine Tortajada, Gregoire Zin & Wiliam Buchmann
In this work, MALDI-MS was evaluated as a method for the study of noncovalent complexes involving DNA and basic polypeptides (penta-L-arginine, penta-L-lysine) or polyamines (polypropylenimine dendrimers, linear and branched polyethylenimines).

Registration Year

  • 2010
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