16 Works

Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers

Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E & Garcia-Roves PM
Permeabilized muscle fibers are extensively used for analysis of mitochondrial function in exercise and pathophysiological studies. Inter- and intra-laboratory comparisons of published results on permeabilized muscle fibers are difficult due to application of different experimental procedures, including sample preparation, substrate-uncoupler-inhibitor titrations (SUIT), respiratory media, and oxygen regimes. Oxygen dependence of mitochondrial respiration in permeabilized fibers (about 100fold higher p50 compared to small living cells and isolated mitochondria [1]) reveals the requirement of using hyperoxic incubation...

Editorial: A vision on preprints

E Gnaiger
A manuscript in preparation for publication on ‘Mitochondrial states and rates’ is the first preprint posted on MitoFit Preprint Archives (Gnaiger et al 2019). It actually triggered the initiation of a preprint server for mitochondrial physiology and bioenergetics. This editorial presents the story behind starting MitoFit Preprint Arch, explaining the rationale of integrating it with the Bioblast and MitoPedia websites, to develop a vision of science communication beyond traditional journal and preprint publication. This is...

Editorial: A vision on preprints

E Gnaiger
A manuscript in preparation for publication on ‘Mitochondrial states and rates’ is the first preprint posted on MitoFit Preprint Archives (Gnaiger et al 2019). It actually triggered the initiation of a preprint server for mitochondrial physiology and bioenergetics. This editorial presents the story behind starting MitoFit Preprint Arch, explaining the rationale of integrating it with the Bioblast and MitoPedia websites, to develop a vision of science communication beyond traditional journal and preprint publication. This is...

Mitochondrial respiratory states and rates

Erich Gnaiger, Eleonor Aasander Frostner, Norwahidah Abdul Karim, Nada A Abumrad, Dario Acuna-Castroviejo, Reginald C Adiele, Bumsoo Ahn, Sameh S Ali, Lesley Alton, Marco G Alves, Francesca Amati, Nivea Dias Amoedo, Ioanna Andreadou, Marc Arago, Cenk Aral, Odeta Arandarcikaite, Anne-Sophie Armand, Thierry Arnould, Vlad Florian Avram, Damian M Bailey, Sudip Bajpeyi, Martina Bajzikova, Barbara M Bakker, Jonathan Barlow, Ana Carolina Bastos Sant'Anna Silva … & Joäo Laranjinha
As the knowledge base and importance of mitochondrial physiology to human health expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow guidelines of the International Union of Pure and Applied...

Mitochondrial respiratory states and rates

Erich Gnaiger, Eleonor Aasander Frostner, Norwahidah Abdul Karim, Nada A Abumrad, Dario Acuna-Castroviejo, Reginald C Adiele, Bumsoo Ahn, Sameh S Ali, Lesley Alton, Marco G Alves, Francesca Amati, Nivea Dias Amoedo, Ioanna Andreadou, Marc Arago, Cenk Aral, Odeta Arandarcikaite, Anne-Sophie Armand, Thierry Arnould, Vlad Florian Avram, Damian M Bailey, Sudip Bajpeyi, Martina Bajzikova, Barbara M Bakker, Jonathan Barlow, Ana Carolina Bastos Sant'Anna Silva … & V Weissig
As the knowledge base and importance of mitochondrial physiology to human health expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow guidelines of the International Union of Pure and Applied...

Interplay between metformin and miR-378a-3p in cells under hyperglycaemia

Machado IF, Teodoro JS, Palmeira CM & Rolo AP
Metformin is commonly used for the treatment of Type 2 Diabetes Mellitus (T2DM) being known for its role in the impairment of hepatic gluconeogenesis. However, it has been associated with the upregulation of microRNAs (miRNAs) involved in T2DM and cancer. Recently, it was reported that metformin promoted the expression of miR-378a-3p in HepG2 cells [1]. This miRNA is embedded in the Ppargc1b gene and has been reported to be an important player in the amelioration...

Itaconic acid decreases mitochondrial respiration and ROS generation in brain tissue

Danielus Umbrasas, T Vanagas, P Cizas & V Borutaite
Itaconic acid (IA) is a recently discovered mammalian metabolite which is produced by macrophages upon pro-inflammatory activation: in quiescent bone marrow – derived macrophages (BMDMs). IA is hardly detectable but upon lipopolysaccharide (LPS) stimulation it reaches millimolar concentrations [1]. Recently reported physiological roles of IA include inhibition of bacterial enzyme isocitrate lyase (bactericidal activity) and the inhibition of a Krebs cycle enzyme succinate dehydrogenase (SDH) in the host cells (which has been shown for BMDMs)...

MitoFit pdfNoncoding RNAs regulatory network in mitochondria

Jusic A, Hajrulahovic A & Devaux Y
Mitochondria are powerhouses of eukaryotic cells and possess own bioenergetically specialized genetic system also known as mitochondrial DNA (mtDNA). In human cells, mtDNA encodes 22 transfer RNAs, 2 ribosomal RNAs and 13 proteins which are subunits of the oxidative phosphorylation machinery. Due to the small size of the mitochondrial genome, mitochondrial biogenesis and function are largely dependent on a number of nuclear-encoded molecules that are imported to mitochondria via the translocase of the outer mitochondrial...

Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism

Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E & Huete-Ortega M
Bioenergetics is the study of how living organisms acquire and transform energy to perform biological work. Energetic coupling between chloroplasts and mitochondria has been described in algae, demonstrating the a good functionality and interaction between both organelles is necessary to maintain metabolic integrity. High-resolution respirometry (HRR) is widely used to assess mitochondrial respiration and other bioenergetics parameters in the biomedical field of mitochondrial research and its clinical applications. In our interdisciplinary study, we adapted the...

Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism

Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E & Huete-Ortega M
Bioenergetics is the study of how living organisms acquire and transform energy to perform biological work. Energetic coupling between chloroplasts and mitochondria has been described in algae, demonstrating the a good functionality and interaction between both organelles is necessary to maintain metabolic integrity. High-resolution respirometry (HRR) is widely used to assess mitochondrial respiration and other bioenergetics parameters in the biomedical field of mitochondrial research and its clinical applications. In our interdisciplinary study, we adapted the...

Mitochondrial respiratory states and rates

Erich Gnaiger, Eleonor Aasander Frostner, Norwahidah Abdul Karim, Nada A Abumrad, Dario Acuna-Castroviejo, Reginald C Adiele, Bumsoo Ahn, Sameh S Ali, Lesley Alton, Marco G Alves, Francesca Amati, Nivea Dias Amoedo, Ioanna Andreadou, Marc Arago, Julian Aragones, Cenk Aral, Odeta Arandarcikaite, Anne-Sophie Armand, Thierry Arnould, Vlad Florian Avram, Damian M Bailey, Sudip Bajpeyi, Martina Bajzikova, Barbara M Bakker, Jonathan Barlow … & Liga Zvejniece
As the knowledge base and importance of mitochondrial physiology to human health expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow guidelines of the International Union of Pure and Applied...

Mitochondrial respiratory states and rates

Erich Gnaiger, Eleonor Aasander Frostner, Norwahidah Abdul Karim, Nada A Abumrad, Dario Acuna-Castroviejo, Reginald C Adiele, Bumsoo Ahn, Sameh S Ali, Lesley Alton, Marco G Alves, Francesca Amati, Nivea Dias Amoedo, Ioanna Andreadou, Marc Arago, Cenk Aral, Odeta Arandarcikaite, Anne-Sophie Armand, Thierry Arnould, Vlad Florian Avram, Damian M Bailey, Sudip Bajpeyi, Martina Bajzikova, Barbara M Bakker, Jonathan Barlow, Ana Carolina Bastos Sant'Anna Silva … & Joäo Laranjinha
As the knowledge base and importance of mitochondrial physiology to human health expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow guidelines of the International Union of Pure and Applied...

Aging effects on mitochondrial control factors in Pink1 knockout Drosophila melanogaster

Debora F Gonçalves, AA Courtes, DD Hartmann, PR Carvalho, DM Marques, ML Machado, AV Furtado, FA Soares & CL Dalla Corte
Parkinson disease (PD) is considered the second most common neurodegenerative disorder in the world and is related mainly with aging. PD is characterized by loss of dopaminergic neurons and formation of protein aggregates, such as α-synuclein [1], leading to motor dysfunction, and impairment of cognitive and memory functions [2]. The pathology and symptoms of PD are well described, although its mechanisms and causes remain unclear. One mechanism involved in PD is mitochondrial dysfunction [3]. Mutation...

Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism

Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E & Huete-Ortega M
Bioenergetics is the study of how living organisms acquire and transform energy to perform biological work. Energetic coupling between chloroplasts and mitochondria has been described in algae, demonstrating the a good functionality and interaction between both organelles is necessary to maintain metabolic integrity. High-resolution respirometry (HRR) is widely used to assess mitochondrial respiration and other bioenergetics parameters in the biomedical field of mitochondrial research and its clinical applications. In our interdisciplinary study, we adapted the...

Effects of atovaquone and 4-nitrobenzoate on Plasmodium falciparum respiration

Crispim M, Verdaguer IB & Katzin AM
Although atovaquone is one of the newest antimalarial compounds discovered, resistant parasites have already been reported1. Atovaquone mechanism of action is established to be the competition with ubiquinol (UQH2) for the bc1 union at mitochondrial cytochrome bc1 complex and preventing the parasite from maintaining an oxidized ubiquinone (UQ) pool, essential for the DHODH activity and consequently for the pyrimidine's biosynthesis. In this sense, possible inhibitors of the ubiquinone biosynthesis pathway would be candidates by stimulating...

Effects of atovaquone and 4-nitrobenzoate on Plasmodium falciparum respiration

Crispim M, Verdaguer IB, Zafra CA & Katzin AM
Although atovaquone is one of the newest antimalarial compounds discovered, resistant parasites have already been reported1. Atovaquone mechanism of action is established to be the competition with ubiquinol (UQH2) for the bc1 union at mitochondrial cytochrome bc1 complex and preventing the parasite from maintaining an oxidized ubiquinone (UQ) pool, essential for the DHODH activity and consequently for the pyrimidine's biosynthesis. In this sense, possible inhibitors of the ubiquinone biosynthesis pathway would be candidates by stimulating...

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