15,082 Works

SapTrap Builder: a desktop utility for CRISPR edit design

Matthew L Schwartz & Erik M Jorgensen
Designing and building DNA reagents for CRISPR edits can be complicated as well as tedious. To streamline reagent design for our SapTrap CRISPR plasmid assembly system, we developed the SapTrap Builder software utility. SapTrap Builder provides an intuitive graphical user interface for visualizing the design of reagents for CRISPR-based genetic tag insertion and point mutagenesis. Our program guides users through several steps of CRISPR edit design, alerts the user when incompatibilities arise, and generates sequences...

Materials Experiment and Analysis Database:

Edwin Soedarmadji, John Gregoire & Contributors

A novel null allele of C. elegans gene ceh-14

Emily A. Bayer & Oliver Hobert
We have generated a novel null allele, ot900, of the C. elegans LIM homeodomain gene ceh-14. All existing deletion alleles of ceh-14 were generated using a Tc1 transposon insertion, one of which, ch3, is a putative null based on the resulting frame-shift and a reduction in detectable mRNA and protein levels (Cassata et al. 2000). However, in our hands we have noticed a propensity of the ch3 allele to revert to wild-type after several generations,...

A new deletion allele of sma-4

Alexandra N. McKillop, Herong Shi & Jun Liu
sma-4 encodes the co-Smad of the BMP pathway in C. elegans, which is also known as the Sma/Mab pathway (Savage et al. 1996). Null mutations in core components of this pathway, including the BMP ligand DBL-1, the receptors SMA-6 and DAF-4, and the R-Smads SMA-2 and SMA-3, all result in a small body size phenotype without significantly compromising viability (Gumienny and Savage-Dunn 2013). However, we found that even after multiple rounds of out-crossing, two deletion...

Materials Experiment and Analysis Database: Material library with W,Cu,Ni,Fe deposited by INKJET on 2013-02-04 from Plate 00004587

Edwin Soedarmadji, John Gregoire & Contributors

Materials Experiment and Analysis Database:

Edwin Soedarmadji, John Gregoire & Contributors

Eph Receptor Clustering by Chondroitin Sulfate Inhibits Axon Regeneration

Gregory Martin Miller
Chondroitin sulfate proteoglycans (CSPGs) play important roles in the developing and mature nervous system, where they guide axons, maintain stable connections, restrict synaptic plasticity, and prevent axon regeneration following CNS injury. The chondroitin sulfate glycosaminoglycan (CS GAG) chains that decorate CSPGs are essential for their functions. Through these sugar chains, CSPGs are able to bind and regulate the activity of a diverse range of proteins and through these interactions can regulate neuronal growth. These CS-protein...

Drosophila balancer reengineering using polycistronic gRNA for CRISPR/Cas9 gene editing

Leif Benner & Brian Oliver
A number of reagents have been generated for CRISPR/Cas9 genome editing in Drosophila (Gratz et al. 2014; Ren et al. 2013; Kondo and Ueda 2013; Port et al. 2014; Port and Bullock 2016). The ability to efficiently express multiple gRNAs from a single vector provides the advantage of creating multiple CRISPR/Cas9 alleles at a time. An elegant design has previously shown that gRNAs separated by tRNAs allow for genomic editing at multiple loci in Drosophila...

Materials Experiment and Analysis Database: Material library with Ti,Fe,Mg,Zr deposited by INKJET on 2013-02-04 from Plate 00004745

Edwin Soedarmadji, John Gregoire & Contributors

Silencing the ASI gustatory neuron pair increases expression of the stress-resistance gene sod-3 in a daf-16 and daf-3 independent manner

Peter Chisnell & Cynthia Kenyon
Ablation of the gustatory neuron pair ASI has been shown to extend lifespan, and this lifespan extension requires the function of the transcription factor daf-16/FOXO (Alcedo and Kenyon 2004). Given this relationship, we tested whether silencing the neuron pair ASI with the tetanus toxin light chain (Tetx), as opposed to ablating it, could increase the expression of a well- known DAF-16 target, the stress-resistance gene sod-3 (Furuyama et al. 2000; Honda and Honda 1999). Tetanus...

TCCON data from East Trout Lake, SK (CA), Release GGG2014.R1

D. Wunch, J. Mendonca, O. Colebatch, N. T. Allen, J.-F. Blavier, S. Roche, J. Hedelius, G. Neufeld, S. Springett, D. Worthy, R. Kessler & K. Strong
The Total Carbon Column Observing Network (TCCON) is a network of ground-based Fourier Transform Spectrometers that record direct solar absorption spectra of the atmosphere in the near-infrared. From these spectra, accurate and precise column-averaged abundances of atmospheric constituents including CO2, CH4, N2O, HF, CO, H2O, and HDO, are retrieved. This data set contains observations from the TCCON station East Trout Lake, SK, Canada.

Materials Experiment and Analysis Database:

Edwin Soedarmadji, John Gregoire & Contributors

Dominant-negative VPS-4 disrupts ODR-10::GFP distribution but has limited effects on chemotaxis

Ellen Zocher, Nelson Ruth & Caroline Dahlberg
Accumulation of ODR-10 depends on the sex and fed state of C. elegans (Ryan et al. 2014), though the precise mechanism of its regulation is not known. We tested whether the ESCRT pathway is required to maintain normal accumulation and distribution of ODR-10::GFP in AWA neurons under well-fed and starved conditions. The conserved AAA ATPase VPS-4 is required as the final step of the ESCRT pathway, to dissociate the machinery as cargo is moved from...

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