Dissertation, ETH Zürich, 2016, No. 23843
OBJECTIVES The purpose of the present study was to investigate whether transfemoral implantation of the balloon-expandable Edwards SAPIEN 3 device without prior balloon valvuloplasty is feasible. BACKGROUND Transcatheter aortic valve implantation (TAVI) without predilatation may be advantageous and is feasible with various transcatheter heart valves. METHODS A total of 163 consecutive patients with severe aortic stenosis undergoing transfemoral TAVI were enrolled at two sites. We assessed whether the crossing of the native aortic valve with...
Suppression of lupus nephritis and skin lesions in MRL/lpr mice by administration of the topoisomerase I inhibitor irinotecan.Ralph Schmid, Andreas Keil, Martin Herrmann, Sean Hall, Meike Körner & Steffen R. Frese
BACKGROUND Since the precise mechanism for the pathogenesis of systemic lupus erythematosus (SLE) is unknown, no targeted therapies in addition to immunosuppression are available so far. We recently demonstrated that administration of the topoisomerase I (topo I) inhibitor irinotecan at extremely low concentrations reversed established lupus nephritis in NZB/NZW mice. While profound immunosuppression was absent, we proposed changes in DNA relaxation and anti-double-stranded (ds)DNA antibody binding as the underlying mechanism. To exclude that these effects...
Effect of recombinant PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix: a pilot study in rat calvarial defects.Roberta Okamoto, Toni Dobsak, Celso K Sonoda, Eloá R Luvizuto, Reinhard Gruber, Stefan Tangl & Karoline Reich
BACKGROUND Supplementation of bone substitutes with recombinant platelet-derived growth factor-BB (PDGF-BB) can enhance bone regeneration. The aim of the study was to evaluate the effect of PDGF-BB on bone formation in the presence of β-tricalcium phosphate and bovine bone mineral matrix in a rat calvaria defect model. METHODS The authors examined 5 mm rat calvarial defects treated with β-tricalcium phosphate (TCP) or demineralized bovine bone mineral (DBBM) with and without 0.3 mg/ml recombinant PDGF-BB. Calvaria defects were...
BACKGROUND High dose radiation therapy is commonly used in maxillofacial surgeries to treat a number of head and neck tumors. Despite its widespread use, little information is available regarding the effects of irradiation on bone cell viability and release of growth factors following dose-dependent irradiation. METHODS Bone samples were collected from porcine mandibular cortical bone and irradiated at doses of 0, 7.5, 15, 30, 60 and 120 Grays. Thereafter, cell viability was quantified, and the...
Discriminative ability of reflex receptive fields to distinguish patients with acute and chronic low back pain.Peter Jüni, Christoph A Agten, Monika Müller, Fabienne Treichel, Paul Heini, José A Biurrun Manresa, Michele Curatolo & Ole K Andersen
Low back pain has a life time prevalence of 70% to 85%. Approximately 10% to 20% of all patients experience recurrent episodes or develop chronic low back pain. Sociodemographic, clinical, and psychological characteristics explain the transition from acute to chronic low back pain only to a limited extent. Altered central pain processing may be a contributing mechanism. The measurement of reflex receptive fields (RRF) is a novel method to assess altered central pain processing. The...
PRINCIPLES Neuroendocrine neoplasms (NENs) are believed to be associated with an increased risk for additional malignancies (AMs). We aimed to (1) assess the occurrence of AM in NEN patients (2) investigate the characteristics and temporal relationship of NEN patients with and without AM. METHODS The SwissNET registry has prospectively documented patients with NEN since 2008, covering the entire area of Switzerland. Clinical characteristics, functionality, location and histology of NEN as well as survival of all...
Modelling the ferrochelatase c.315-48C modifier mutation for erythropoietic protoporphyria (EPP) in miceJasmin Barman-Aksözen, Daniel Schümperli, Paolo Cinelli, Judith Trüb, Elisabeth I. Minder, Vijay B. Bansode, Xiaoye Schneider-Yin, Pawel Pelczar, Paulina Ćwiek & Frank Koentgen
Erythropoietic Protoporphyria (EPP) is caused by deficiency of ferrochelatase (FECH) which incorporates iron into protoporphyrin IX (PPIX) to form heme. Excitation of accumulated PPIX by light generates oxygen radicals which evoke excessive pain and, after longer light exposure, ulcerations in exposed skin areas of EPP patients. Moreover, ∼5% of the patients develop a liver dysfunction due to PPIX accumulation. Most patients (∼97%) have a severe FECH mutation (Mut) in trans to an intronic polymorphism (c.315-48C)...
Prolonged pemetrexed pretreatment augments persistence of cisplatin-induced DNA damage and eliminates resistant lung cancer stem-like cells associated with EMT.Colin Tièche, Ralph Schmid, Patrick Dorn, Laurène Froment, Thomas Marti & Ren-Wang Peng
BACKGROUND Lung cancer is the leading cause of cancer-related mortality, and new therapeutic options are urgently needed. Non-small cell lung cancer (NSCLC) accounts for approximately 85% of all lung cancers, with the current standard regimen of care for NSCLC including chemotherapy with pemetrexed as a single agent or in combination with platinum-based agents, e.g. cisplatin. Pemetrexed is a folic acid antagonist that inhibits the synthesis of precursor nucleotides, whereas cisplatin directly induces DNA adducts, the...
In vitro characterization of an osteoinductive biphasic calcium phosphate in combination with recombinant BMP2.Richard John Miron, Masako Kobayashi, Yufeng Zhang, Yang Shuang, Anton Sculean & Lin Yizhen
BACKGROUND The repair of alveolar bone defects with growth factors and bone grafting materials has played a pivotal role in modern dentistry. Recombinant human bone morphogenetic protein-2 (rhBMP2), an osteoinductive growth factor capable of cell recruitment and differentiation towards the osteoblast lineage, has been utilized in combination with various biomaterials to further enhance new bone formation. Recently, a group of novel biphasic calcium phosphate (BCP) bone grafting materials have been demonstrated to possess osteoinductive properties...
Schedule-dependent increased efficiency of pemetrexed-ionizing radiation combination therapy elicits a differential DNA damage response in lung cancer cells.Colin Tièche, Thomas Marti, Ralph Schmid, Patrick Dorn, Laurène Froment & Ren-Wang Peng
BACKGROUND Lung cancer causes the most cancer deaths worldwide, thus there is a urgent need to develop new treatment options. Concurrent chemoradiotherapy has become a common strategy for the treatment of non-resectable solid tumors including non-small cell lung cancer. Pemetrexed is a folic acid antagonist that inhibits the synthesis of precursor nucleotides, whereas ionizing radiation induces DNA damage, the repair of which is dependent on sufficiently high nucleotide levels. In the clinical setting, the pemetrexed-ionizing...
Distribution and surface enrichment of radionuclides in lead-bismuth eutectic from spallation targetsJörg Neuhausen, David, J. -C., L. Zanini, Viktor Boutellier, Bernadette Hammer-Rotzler, Michael Wohlmuther, Andreas Türler & Dorothea Schumann
With the development of new high-power neutron spallation sources --both for scientific application and as neutron production tool for accelerator-driven systems-- the demand for experimentally obtained nuclear data on the residue nuclei production in the target is constantly increasing. In the present work, we examined two lead-bismuth-eutectic targets, irradiated with high-energy protons, concerning their radionuclide content and the spatial distribution of selected isotopes. The first one was the so-called ISOLDE target, being irradiated with 1-1.4GeV...
Salivary pellets induce a pro-inflammatory response involving the TLR4-NF-kB pathway in gingival fibroblasts.Adrian Lussi, Heinz-Dieter Müller, Barbara Cvikl & Reinhard Gruber
BACKGROUND Whole saliva provokes a substantial pro-inflammatory response in gingival fibroblasts. This raises the question whether the salivary pellet, which is used for diagnostic purposes, also has a pro-inflammatory capacity and, if yes, what the underlying mechanisms at the molecular level are. METHODS We examined the ability of extensively washed salivary pellets to provoke the expression of chemokines in gingival fibroblasts by real-time polymerase chain reaction and immunoassays. Protein composition was determined with proteomic analysis....
Hair cortisol as a hypothalamic-pituitary-adrenal axis biomarker in pregnant women with asthma: a retrospective observational study.Anne Smith, Gideon Koren, Howard Berger, Kaitlyn Shaw, Laura Smy, Bruce Carleton & Ursula Amstutz
BACKGROUND Cortisol is a hormone involved in many physiological functions including fetal maturation and epigenetic programming during pregnancy. This study aimed to use hair cortisol as a biomarker of chronic inhaled corticosteroid (ICS) exposure and assess the potential effects of asthma on the hypothalamic-pituitary-adrenal (HPA) axis in pregnant women. We hypothesized that pregnant women with asthma treated with ICS would exhibit lower hair cortisol concentrations, indicative of adrenal suppression, compared to women with asthma not...
Association of erosive tooth wear and dental caries in Northern Finland Birth Cohort 1966 - an epidemiological cross-sectional study.Paula Pesonen, Adrian Lussi, Vuokko Anttonen, Leo Tjäderhane, Viivi Alaraudanjoki & Marja-Liisa Laitala
BACKGROUND The main aim of this cross-sectional study was to investigate the prevalence and severity of erosive tooth wear and its association with dental caries and socio-demographic factors among middle-aged Finnish adults. METHODS Of the total Northern Finland Birth Cohort 1966 (n = 12,058), a convenience sample (n = 3181 adults) was invited for an oral health examination of which 1962 (61.7 %) participated, comprising the final study group. Clinical examinations were carried out by trained and calibrated dentists. Erosive...
Bone conditioned media (BCM) improves osteoblast adhesion and differentiation on collagen barrier membranes.Masako Kobayashi, Richard John Miron, Daniel Buser, Jordi Caballé Serrano, Dieter Bosshardt & Reinhard Gruber
BACKGROUND The use of autogenous bone chips during guided bone regeneration procedures has remained the gold standard for bone grafting due to its excellent combination of osteoconduction, osteoinduction and osteogenesis. Recent protocols established by our group have characterized specific growth factors and cytokines released from autogenous bone that have the potential to be harvested and isolated into bone conditioned media (BCM). Due to the advantageous osteo-promotive properties of BCM, the aims of the present study...
BACKGROUND Spontaneous whole lung torsion is an absolut rarity and most cases occur after previous surgery. CASE PRESENTATION We present the case of a spontaneous whole-lung torsion in a 82-year old man. The patient was referred to our thoracic surgery department from the emergency department of a referring hospital with rapidly progressive dyspnea. CT-scan revealed a 180° degree counterclockwise torsion of the entire right lung with complete atelectasis and congestion of the upper lobe as...
Clinical and epidemiological analysis of Campylobacter fetus subsp. fetus infections in humans and comparative genetic analysis with strains isolated from cattle.Carlos Abril Gaona, Sara Christine Droz, Isabelle Brodard, Colette Brunner, Niklaus Von Steiger, Peter Kuhnert & Robert Escher
BACKGROUND Campylobacter fetus subspecies fetus (CFF) is an important pathogen for both cattle and humans. We performed a systematic epidemiological and clinical study of patients and evaluated the genetic relatedness of 17 human and 17 bovine CFF isolates by using different genotyping methods. In addition, the serotype, the dissemination of the genomic island containing a type IV secretion system (T4SS) and resistance determinants for tetracycline and streptomycin were also evaluated. METHODS The isolates from patients...
Universität Zürich, ZORA55,788
E-Pics 4, Biosys43,838
Bern Open Repository and Information System (BORIS)22,364
E-Pics Alte und Seltene Drucke15,362
E-Pics 6, Sternwarte5,648
edoc - University Library of Basel2,485
E-Pics 3, EWS2,465