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Appendix A

Aditya Pratama
The behaviour of bucket brigades with worker collaboration on 3-station, 2-worker line with fully or partially cross-trained workers as slowest-to-fastest and fastest-to-slowest can be derived as follows:

Coral-hydrozoan co-occurrence dataset from Corals hosting symbiotic hydrozoans are less susceptible to predation and disease

Simone Montano, Simone Fattorini, Valeriano Parravicini, Michael L. Berumen, Paolo Galli, Davide Maggioni, Roberto Arrigoni, Davide Seveso & Giovanni Strona
The dataset report detailed information about all coral colonies investigated in the study. This includes sampling region (Maldives or Saudi Arabia), a unique identifier for sampling site, depth class, coral genus, colony size class, a binary field indicating the occurrence on the colony of symbiotic hydrozoans, and 8 binary fields indicating the presence of signs of various coral health conditions, namely: coral bleaching, coral algae overgrowth, fish bites, gastropod (Drupella spp.) bites, white syndrome, brown...

Supplementary material from "Corals hosting symbiotic hydrozoans are less susceptible to predation and disease"

Simone Montano, Simone Fattorini, Valeriano Parravicini, Michael L. Berumen, Paolo Galli, Davide Maggioni, Roberto Arrigoni, Davide Seveso & Giovanni Strona
In spite of growing evidence that climate change may dramatically affect networks of interacting species, whether—and to what extent—ecological interactions can mediate species' response to disturbances is an open question. Here we show how a largely overseen association such as that between hydrozoans and scleractinian corals could be possibly associated with a reduction in coral susceptibility to ever-increasing predator and disease outbreaks. We examined 2450 scleractinian colonies (from both Maldivian and the Saudi Arabian coral...

Enhancement of mechanical and tribological properties of SiC- and CB-reinforced aluminium 7075 hybrid composites through friction stir processing

Pratip Roy, Subhash Singh & Kaushik Pal
Aluminium alloy has wide application in both aircraft and automobile industry due to its high strength and light weight benefits. In this research work, stir casting process was used for fabricating aluminium alloy 7075 reinforced by silicon carbide (SiC) and carbon black (CB) particulates. X-ray diffraction analysis, energy dispersive X-Rays analysis and field emission scanning electron microscopy (FE-SEM) confirmed the presence of SiC and CB particles and there uniform distribution in aluminium matrix. Further, as-cast...

Supplement-Appendix A.docx

Aditya Pratama
The convergence condition of bucket brigades with worker collaboration with fully and partially cross-trained workers under a three-workstation, two-worker production line

Supplementary material from “Atlantic bluefin tuna spawn at suboptimal temperatures for their offspring”

P. Reglero, A. Ortega, R. Balbín, F.J. Abascal, A. Medina, E. Blanco, F. De La Gándara, D. Alvarez-Berastegui, M. Hidalgo, L. Rasmuson, F. Alemany & Ø. Fiksen
Supplementary material from “Atlantic bluefin tuna spawn at suboptimal temperatures for their offspring”

Supplementary material from "Atlantic bluefin tuna spawn at suboptimal temperatures for their offspring"

P. Reglero, A. Ortega, R. Balbín, F.J. Abascal, A. Medina, E. Blanco, F. De La Gándara, D. Alvarez-Berastegui, M. Hidalgo, L. Rasmuson, F. Alemany & Ø. Fiksen
Life-history traits such as spawning migrations and timing of reproduction are adaptations to specific environmental constraints and seasonal cycles in many organisms' annual routines. In this study we analyse how offspring fitness constrains spawning phenology in a large migratory apex predator, the Atlantic bluefin tuna. The reproductive schedule of Atlantic bluefin tuna varies between spawning sites suggesting plasticity to local environmental conditions. Generally, temperature is considered to be the main constraint on tuna spawning phenology....

Supplementary material from "Atlantic bluefin tuna spawn at suboptimal temperatures for their offspring"

P. Reglero, A. Ortega, R. Balbín, F.J. Abascal, A. Medina, E. Blanco, F. De La Gándara, D. Alvarez-Berastegui, M. Hidalgo, L. Rasmuson, F. Alemany & Ø. Fiksen
Life-history traits such as spawning migrations and timing of reproduction are adaptations to specific environmental constraints and seasonal cycles in many organisms' annual routines. In this study we analyse how offspring fitness constrains spawning phenology in a large migratory apex predator, the Atlantic bluefin tuna. The reproductive schedule of Atlantic bluefin tuna varies between spawning sites suggesting plasticity to local environmental conditions. Generally, temperature is considered to be the main constraint on tuna spawning phenology....

Supplementary Result Tables from Strong social relationships are associated with decreased longevity in a facultatively social mammal

Daniel T. Blumstein, Dana M. Williams, Alexandra N. Lim, Svenja Kroeger & Julien G. A. Martin
Humans in strong social relationships are more likely to live longer because social relationships may buffer stressors and thus have protective effects. However, a shortcoming of human studies is that they often rely on self-reporting of these relationships. By contrast, observational studies of non-human animals permit detailed analyses of the specific nature of social relationships. Thus, discoveries that some social animals live longer and healthier lives if they are involved in social grooming, forage together...

Supplementary material from "Strong social relationships are associated with decreased longevity in a facultatively social mammal"

Daniel T. Blumstein, Dana M. Williams, Alexandra N. Lim, Svenja Kroeger & Julien G.A. Martin
Humans in strong social relationships are more likely to live longer because social relationships may buffer stressors and thus have protective effects. However, a shortcoming of human studies is that they often rely on self-reporting of these relationships. By contrast, observational studies of non-human animals permit detailed analyses of the specific nature of social relationships. Thus, discoveries that some social animals live longer and healthier lives if they are involved in social grooming, forage together...

Supplementary material from "Strong social relationships are associated with decreased longevity in a facultatively social mammal"

Daniel T. Blumstein, Dana M. Williams, Alexandra N. Lim, Svenja Kroeger & Julien G.A. Martin
Humans in strong social relationships are more likely to live longer because social relationships may buffer stressors and thus have protective effects. However, a shortcoming of human studies is that they often rely on self-reporting of these relationships. By contrast, observational studies of non-human animals permit detailed analyses of the specific nature of social relationships. Thus, discoveries that some social animals live longer and healthier lives if they are involved in social grooming, forage together...

Mechanochemically-generated solid state complex of C60-fullerene with tetra-(5,7-diphenyl)calix[4]azulene, NMR, XRD and DFT studies

Celine Schneider, Hidetaka Nishimura, Jaehyun Lee, Lawrence T. Scott, Atsushi Wakamiya, Roy Forbes & Paris E. Georghiou
The synthesis of tetra-(5,7-diphenyl)calix[4]azulene via the reaction of 5,7-diphenylazulene with formaldehyde is described. Its solid-state supramolecular complexation with C60 produced by a grinding of the two components was evaluated by solid-state 13C-NMR spectroscopy and X-ray powder diffraction. Gas-phase DFT studies of possible structures are reported.

Additional file 2: of Myocardial inflammation, injury and infarction during on-pump coronary artery bypass graft surgery

Shirjel Alam, Colin Stirrat, Nick Spath, Vipin Zamvar, Renzo Pessotto, Marc Dweck, Colin Moore, Scott Semple, Ahmed El-Medany, Divya Manoharan, Nicholas Mills, Anoop Shah, Saeed Mirsadraee, David Newby & Peter Henriksen
Correlations and change in ejection fraction (pre to post surgery). (DOCX 176 kb)

Myocardial inflammation, injury and infarction during on-pump coronary artery bypass graft surgery

Shirjel Alam, Colin Stirrat, Nick Spath, Vipin Zamvar, Renzo Pessotto, Marc Dweck, Colin Moore, Scott Semple, Ahmed El-Medany, Divya Manoharan, Nicholas Mills, Anoop Shah, Saeed Mirsadraee, David Newby & Peter Henriksen
Abstract Background Myocardial inflammation and injury occur during coronary artery bypass graft (CABG) surgery. We aimed to characterise these processes during routine CABG surgery to inform the diagnosis of type 5 myocardial infarction. Methods We assessed 87 patients with stable coronary artery disease who underwent elective CABG surgery. Myocardial inflammation, injury and infarction were assessed using plasma inflammatory biomarkers, high-sensitivity cardiac troponin I (hs-cTnI) and cardiac magnetic resonance imaging (CMR) using both late gadolinium enhancement...

Myocardial inflammation, injury and infarction during on-pump coronary artery bypass graft surgery

Shirjel Alam, Colin Stirrat, Nick Spath, Vipin Zamvar, Renzo Pessotto, Marc Dweck, Colin Moore, Scott Semple, Ahmed El-Medany, Divya Manoharan, Nicholas Mills, Anoop Shah, Saeed Mirsadraee, David Newby & Peter Henriksen
Abstract Background Myocardial inflammation and injury occur during coronary artery bypass graft (CABG) surgery. We aimed to characterise these processes during routine CABG surgery to inform the diagnosis of type 5 myocardial infarction. Methods We assessed 87 patients with stable coronary artery disease who underwent elective CABG surgery. Myocardial inflammation, injury and infarction were assessed using plasma inflammatory biomarkers, high-sensitivity cardiac troponin I (hs-cTnI) and cardiac magnetic resonance imaging (CMR) using both late gadolinium enhancement...

Discovery of the fourth mobile sulfonamide resistance gene

Mohammad Razavi, Nachiket Marathe, Michael Gillings, Carl-Fredrik Flach, Erik Kristiansson & D. Joakim Larsson
Abstract Background Over the past 75 years, human pathogens have acquired antibiotic resistance genes (ARGs), often from environmental bacteria. Integrons play a major role in the acquisition of antibiotic resistance genes. We therefore hypothesized that focused exploration of integron gene cassettes from microbial communities could be an efficient way to find novel mobile resistance genes. DNA from polluted Indian river sediments were amplified using three sets of primers targeting class 1 integrons and sequenced by long-...

Discovery of the fourth mobile sulfonamide resistance gene

Mohammad Razavi, Nachiket Marathe, Michael Gillings, Carl-Fredrik Flach, Erik Kristiansson & D. Joakim Larsson
Abstract Background Over the past 75 years, human pathogens have acquired antibiotic resistance genes (ARGs), often from environmental bacteria. Integrons play a major role in the acquisition of antibiotic resistance genes. We therefore hypothesized that focused exploration of integron gene cassettes from microbial communities could be an efficient way to find novel mobile resistance genes. DNA from polluted Indian river sediments were amplified using three sets of primers targeting class 1 integrons and sequenced by long-...

Additional file 9: of Discovery of the fourth mobile sulfonamide resistance gene

Mohammad Razavi, Nachiket Marathe, Michael Gillings, Carl-Fredrik Flach, Erik Kristiansson & D. Joakim Larsson
Phylogenetic tree of DHPS proteins encoded by chromosomal genes and mobile sulfonamide resistance genes. (TXT 283 kb)

Additional file 1: of Myocardial inflammation, injury and infarction during on-pump coronary artery bypass graft surgery

Shirjel Alam, Colin Stirrat, Nick Spath, Vipin Zamvar, Renzo Pessotto, Marc Dweck, Colin Moore, Scott Semple, Ahmed El-Medany, Divya Manoharan, Nicholas Mills, Anoop Shah, Saeed Mirsadraee, David Newby & Peter Henriksen
Systemic inflammatory markers. (DOCX 387 kb)

Additional file 8: of Discovery of the fourth mobile sulfonamide resistance gene

Mohammad Razavi, Nachiket Marathe, Michael Gillings, Carl-Fredrik Flach, Erik Kristiansson & D. Joakim Larsson
Phylogenetic tree of the identified OXA-variant gene cassettes and 289 known OXA-variants retrieved from the CARD database. (TXT 9 kb)

DNA and RNA-sequence based GWAS highlights membrane-transport genes as key modulators of milk lactose content

Thomas Lopdell, Kathryn Tiplady, Maksim Struchalin, Thomas Johnson, Michael Keehan, Ric Sherlock, Christine Couldrey, Stephen Davis, Russell Snell, Richard Spelman & Mathew Littlejohn
Abstract Background Lactose provides an easily-digested energy source for neonates, and is the primary carbohydrate in milk in most species. Bovine lactose is also a key component of many human food products. However, compared to analyses of other milk components, the genetic control of lactose has been little studied. Here we present the first GWAS focussed on analysis of milk lactose traits. Results Using a discovery population of 12,000 taurine dairy cattle, we detail 27...

DNA and RNA-sequence based GWAS highlights membrane-transport genes as key modulators of milk lactose content

Thomas Lopdell, Kathryn Tiplady, Maksim Struchalin, Thomas Johnson, Michael Keehan, Ric Sherlock, Christine Couldrey, Stephen Davis, Russell Snell, Richard Spelman & Mathew Littlejohn
Abstract Background Lactose provides an easily-digested energy source for neonates, and is the primary carbohydrate in milk in most species. Bovine lactose is also a key component of many human food products. However, compared to analyses of other milk components, the genetic control of lactose has been little studied. Here we present the first GWAS focussed on analysis of milk lactose traits. Results Using a discovery population of 12,000 taurine dairy cattle, we detail 27...

Canary: an atomic pipeline for clinical amplicon assays

Kenneth Doig, Jason Ellul, Andrew Fellowes, Ella Thompson, Georgina Ryland, Piers Blombery, Anthony Papenfuss & Stephen Fox
Abstract Background High throughput sequencing requires bioinformatics pipelines to process large volumes of data into meaningful variants that can be translated into a clinical report. These pipelines often suffer from a number of shortcomings: they lack robustness and have many components written in multiple languages, each with a variety of resource requirements. Pipeline components must be linked together with a workflow system to achieve the processing of FASTQ files through to a VCF file of...

Canary: an atomic pipeline for clinical amplicon assays

Kenneth Doig, Jason Ellul, Andrew Fellowes, Ella Thompson, Georgina Ryland, Piers Blombery, Anthony Papenfuss & Stephen Fox
Abstract Background High throughput sequencing requires bioinformatics pipelines to process large volumes of data into meaningful variants that can be translated into a clinical report. These pipelines often suffer from a number of shortcomings: they lack robustness and have many components written in multiple languages, each with a variety of resource requirements. Pipeline components must be linked together with a workflow system to achieve the processing of FASTQ files through to a VCF file of...

Additional file 6 of DNA and RNA-sequence based GWAS highlights membrane-transport genes as key modulators of milk lactose content

Thomas Lopdell, Kathryn Tiplady, Maksim Struchalin, Thomas Johnson, Michael Keehan, Ric Sherlock, Christine Couldrey, Stephen Davis, Russell Snell, Richard Spelman & Mathew Littlejohn
Figure S3. WGS resolution for eQTL of all gene located within 1Mbp windows centred on QTL peaks for lactose phenotypes. (PDF 8428 kb)

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