69 Works

Active Site-labeled Prothrombin Inhibits Prothrombinase in Vitro and Thrombosis in Vivo

Heather K. Kroh, Peter Panizzi, Svetlana Tchaikovski, T. Regan Baird, Nancy Wei, Sriram Krishnaswam, Guido Tans, Jan Rosing, Bruce Furie, Barbara C. Furie & Paul E. Bock
Mouse and human prothrombin (ProT) active site specifically labeled with D-Phe-Pro-Arg-CH2Cl (FPR-ProT) inhibited tissue factor-initiated thrombin generation in platelet-rich and platelet-poor mouse and human plasmas. FPR-prethrombin 1 (Pre 1), fragment 1 (F1), fragment 1.2 (F1.2), and FPR-thrombin produced no significant inhibition, demonstrating the requirement for all three ProT domains. Kinetics of inhibition of ProT activation by the inactive ProTS195A mutant were compatible with competitive inhibition as an alternate nonproductive substrate, although FPR-ProT deviated from this...

The P2Y(2) Nucleotide Receptor Mediates Tissue Factor Expression in Human Coronary Artery Endothelial Cells

Ling Ding, Wanshu Ma, Timothy Littmann, Riley Camp & Jianzhong Shen
The discovery of the role of P2Y12 receptor in platelet aggregation leads to a new anti-thrombotic drug Plavix; however, little is known about non-platelet P2Y receptors in thrombosis. This study tested the hypothesis that endothelial P2Y receptor(s) mediates up-regulation of tissue factor (TF), the initiator of coagulation cascade. Stimulation of human coronary artery endothelial cells (HCAEC) by UTP/ATP increased the mRNA level of TF but not of its counterpart-tissue factor pathway inhibitor, which was accompanied...

Combining Hardwood and Softwood Lignocellulosic Biomass for the Production of Ethanol

Angela Carver

Interpretation of the photoelectron spectra of superalkali species: Na3O and Na3O-

S Zein, J. V. Ortiz & Joseph Vincent Ortiz
Recently measured photoelectron spectra of the Na3O- anion have been interpreted with the aid of ab initio electron propagator calculations. As in the case of the Li3O-, we propose that the photoionization of ground and excited neutral states, in a sequential two photon absorption mechanism, plays a role in the interpretation of the observed spectrum. The lowest vertical electron detachment energy of Na3O- corresponds to a Dyson orbital that is composed chiefly of diffuse Na...

Mindfulness to Manage Workplace Stress and Microaggressions

Chippewa Thomas, Pambanisha Whaley & Jaena Alabi
Navigating workplace stress and microaggressions—verbal and non-verbal messages that are invalidating or demeaning—can be difficult in any profession, but is especially challenging in service-oriented professions like librarianship. In addition to identifying and naming stressors and microaggressions, various tools can be employed to mitigate the negative effects of these encounters. This session will provide attendees with a framework—mindfulness—that can be used to help process the often-fraught interactions that may take place in our libraries. The webinar...

Geophagy of Yunnan snub-nosed monkeys (Rhinopithecus bieti) at Xiangguqing in the Baimaxueshan Nature Reserve, China

Dayong Li, Baoping Ren, Jie Hu, Qingyu Zhang, Yumin Yang, Cyril C. Grueter, Ali Krzton, Xinming He, Ming Li & Alicia Krzton
Geophagy is common in extant nonhuman primate species, but the exact reasons for it across species remain unclear. Previous diet studies on Yunnan snub-nosed monkeys (Rhinopithecus bieti) were only focused on organic materials (plants and small animals). There are no reports on R. bieti exhibiting geophagy in the field before this study. This study was carried out at Xiangguqing in the Baimaxueshan Nature Reserve from June 2008 to May 2009. We recorded the behavior of...

Genomic responses to selection for tame/aggressive behaviors in the silver fox (Vulpes vulpes)

Xu Wang, Lenore Pipes, Lyudmila N. Trut, Yury Herbeck, Anastasiya V. Vladimirova, Rimma G. Gulevich, Anastasiya V. Kharlamova, Jennifer L. Johnson, Gregory M. Acland, Anna V. Kukekova & Andrew G. Clark
Animal domestication efforts have led to a shared spectrum of striking behavioral and morphological changes. To recapitulate this process, silver foxes have been selectively bred for tame and aggressive behaviors for more than 50 generations at the Institute for Cytology and Genetics in Novosibirsk, Russia. To understand the genetic basis and molecular mechanisms underlying the phenotypic changes, we profiled gene expression levels and coding SNP allele frequencies in two brain tissue specimens from 12 aggressive...

A Remodeled Protein Arginine Methyltransferase 1 (PRMT1) Generates Symmetric Dimethylarginine

Shanying Gui, Symon Gathiaka, Jun Li, Jun Qu, Orlando Acevedo & Joan M. Hevel
Protein arginine methylation is emerging as a significant post-translational modification involved in various cell processes and human diseases. As the major arginine methylation enzyme, protein arginine methyltransferase 1 (PRMT1) strictly generates monomethylarginine and asymmetric dimethylarginine (ADMA), but not symmetric dimethylarginine (SDMA). The two types of dimethylarginines can lead to distinct biological outputs, as highlighted in the PRMT-dependent epigenetic control of transcription. However, it remains unclear how PRMT1 product specificity is regulated. We discovered that a...

Delocalized water and fluoride contributions to Dyson orbitals for electron detachment from the hydrated fluoride anion

Sylvio Canuto, Kaline Coutinho, Benedito J. C. Cabral, V. G. Zakrzewski & J.V. Ortiz
The experimental vertical electron detachment energy VEDE of aqueous fluoride, F− H2O , is approximately 9.8 eV, but spectral assignment is complicated by interference between F− 2p and H2O 1b1 orbitals. The electronic structure of F− H2O is analyzed with Monte Carlo and ab initio quantum-mechanical calculations. Electron-propagator calculations in the partial third-order approximation yield a VEDE of 9.4 eV. None of the Dyson orbitals corresponding to valence VEDEs consists primarily of F 2p functions....

Indirect Impacts of Recreational Scuba Diving: Patterns of Growth and Predation in Branching Stony Corals

Barak Guzner, Ariel Novplansky, Or Shalit & Nanette E. Chadwick
The highest recorded rates of recreational SCUBA diving occur on coral reefs at Eilat in the northern Red Sea, where many scleractinian corals are damaged by physical contact with divers. We compared coral skeletal growth and damage at sites that were protected vs unprotected from diving tourism, and identified tissue mortality through monthly monitoring of the branching stony coral Acropora hemprichii (Ehrenberg, 1834). We also monitored several genera of branching stony corals at sites that...

Electron propagator and coupled-cluster calculations on the photoelectron spectra of thiouracil and dithiouracil anions

O. Dolgounitcheava, V. G. Zakrzewski & J. V. Ortiz
Electron affinities, vertical electron detachment energies, and isomerization energies of 4-thiouracil, 2-thiouracil, and 2,4-dithiouracil and their valence anions have been calculated with ab initio electron propagator and other many-body methods. Anions in which protons have been transferred to the C5 from the N1 or N3 ring positions have been considered, but the canonical forms are most stable for the 4-thiouracil and 2,4-dithiouracil anions. Electron affinities of 0.61, 0.26, and 0.87 eV have been determined for...

Quasiparticle virtual orbitals in electron propagator calculations

Roberto Flores-Moreno & J.V. Ortiz
The computational limits of accurate electron propagator methods for the calculation of electron binding energies of large molecules are usually determined by the rank of the virtual orbital space. Electron density difference matrices that correspond to these transition energies in the second-order quasiparticle approximation may be used to obtain a virtual orbital space of reduced rank that introduces only minor deviations with respect to the results produced with the full, original set of virtual orbitals....

Efficient evaluation of analytic Fukui functions

Roberto Flores-Moreno, Junia Melin, J.V. Ortiz & Gabriel Merino
An efficient method for the analytic evaluation of Fukui functions is proposed. Working equations are derived and numerical results are used to validate the method on medium size set of molecules. In addition to the obvious advantages of analytic differentiation, the proposed method is efficient enough to be considered a practical alternative to the finite difference formulation used routinely. The reliability of the approximations used here is demonstrated and discussed. Problems found in other methods...

Assessment of transition operator reference states in electron propagator calculations

Roberto Flores-Moreno, V. G. Zakrzewski & J.V. Ortiz
The transition operator method combined with second-order, self-energy corrections to the electron propagator TOEP2 may be used to calculate valence and core-electron binding energies. This method is tested on a set of molecules to assess its predictive quality. For valence ionization energies, well known methods that include third-order terms achieve somewhat higher accuracy, but only with much higher demands for memory and arithmetic operations. Therefore, we propose the use of the TOEP2 method for the...

Electron binding energies and Dyson orbitals of Al(5)O(m)(-) (m=3,4,5) and Al(5)O(5)H(2)(-)

Alfredo Guevara-Garcia, Ana Martinez & J.V. Ortiz
Photoelectron spectra of Al5Om − m=3–5 and of the anion produced by the dissociative adsorption of a water molecule by Al5O4 − are interpreted with density-functional geometry optimizations and electron-propagator calculations of vertical electron detachment energies. For Al5O3 − , Al5O4 − , and Al5O5H2 − , the observed signals may be attributed to the most stable isomer of each anion. For Al5O5 − , the features in the photoelectron spectrum are due to three...

Performance of ryegrass varieties in Alabama, 2001-2002

Kathryn M. Glass & Edzard Van Santen
"July 2002."

Estimating the Center of Rotation of Tomographic Imaging Systems with Limited Projections

Huanyi Zhou, Stanley J. Reeves & Peter R. Panizzi
For a tomographic imaging system, image reconstruction quality is highly correlated with accurate determination of the true center of rotation (COR) location. A significant center offset error will introduce ringing, streaking, or other artifacts into the reconstruction, while smaller COR error will cause blurring of the image. Well known COR correction techniques including image registration, center of mass calculation, or reconstruction evaluation work well under certain conditions. However, some conditions, e.g. parallel projections or no...

Identification of Rickettsia felis DNA in the blood of domestic cats and dogs in the USA

, Subarna Barua, Patrick John Kelly, Chenoweth Kelly, Bernhard Kaltenboeck & Chengming Wang
Background: The main vector and reservoir host of Rickettsia felis, an emerging human pathogen causing flea-borne spotted fever, is the cat flea Ctenocephalides felis. While cats have not been found to be infected with the organism, significant percentages of dogs from Australia and Africa are infected, indicating that they may be important mammalian reservoirs. The objective of this study was to determine the presence of R. felis DNA in the blood of domestic dogs and...

Is Pairing with a Relative Heritable? Estimating Female and Male Genetic Contributions to the Degree of Biparental Inbreeding in Song Sparrows (Melospiza melodia)

Mathew E. Wolak & Jane M. Reid
The degree of inbreeding expressed within populations can profoundly shape evolutionary dynamics. The degree to which individuals inbreed is frequently assumed to evolve in response to selection, for example, resulting from inbreeding depression. Such evolutionary responses require additive genetic variance (V-A) in the degree to which individuals inbreed. However, the magnitude of V-A in the degree of biparental inbreeding has never been estimated. We devised a quantitative genetic model to estimate sex-specific V-A in the...

Microaggressions, harassment, and workplace stress

Chippewa Thomas, Pambanisha Whaley & Jaena Alabi
A panel presentation about microaggressions, harassment, which can contribute to workplace stress. Potential strategies for mitigating the negative effects of experiencing these phenomena are also presented. Part of the REFORMA VII pre-conference, "Bridging the Gap: In Support of Women in Librarianship."

It Takes a Village: Incorporating Intentionality Into Network Development

Jennilyn M. Wiley
Do you have problems connecting with colleagues that can answer your questions? Do you ever find yourself in a situation where you aren’t sure who can address a particular knowledge gap? Do you expect network development activities to occur organically? Have you ever wondered what a sociogram is or how to create one? If you answered yes to any of these questions, come discover the process of social network analysis (SNA) and how you can...

Interpretation of the photoelectron spectra of superalkali species: Li3O and Li3O-

S. Zein & J. V. Ortiz
The present paper deals with the interpretation of the photoelectron spectrum of the Li3O-. After several failed attempts to attribute all of the observed peaks in the experimental spectrum to anionic species, neutral species were considered assuming a sequential two-photon absorption mechanism. We find that only two of the six observed peaks can be attributed to photodetachments and that all other observed features can be assigned to ionizations from the ground and excited states of...

Performance of cotton varieties in Alabama, 2020

Henry G. Jordan
Caption title. "January 11, 2021."

OH3- and O2H5- double Rydberg anions: Predictions and comparisons with NH4- and N2H7-

Junia Melin & J.V. Ortiz
A low barrier in the reaction pathway between the double Rydberg isomer of OH3 − and a hydride-water complex indicates that the former species is more difficult to isolate and characterize through anion photoelectron spectroscopy than the well known double Rydberg anion DRA, tetrahedral NH4 − . Electron propagator calculations of vertical electron detachment energies VEDEs and isosurface plots of the electron localization function disclose that the transition state’s electronic structure more closely resembles that...

Wild Pigs: inciting factor in southern pine decline?

Lori G. Eckhardt, Roger D. Menard & Stephen S. Ditchkoff
During an investigation into southern pine decline at Fort Benning Georgia, the possibility of wild pigs (Sus scrofa) as an inciting factor became evident. Their rooting activity caused significant root damage on sites showing symptoms of pine decline. It was thought that perhaps the pigs may be moving around pathogenic fungi during their rooting activity in Pinus taeda (loblolly pine) stands. In 2008 and 2010, fungal isolates were obtained from the snouts of wild pigs...

Registration Year

  • 2021
  • 2020

Resource Types

  • Text