7 Works

Live imaging of remyelination in the adult mouse corpus callosum

Sebastian Jessberger & Sara Bottes
Oligodendrocyte precursor cells (OPCs) retain the capacity to remyelinate axons in the corpus callosum (CC) upon demyelination. However, the dynamics of OPC activation, mode of cell division, migration, and differentiation on a single cell level remain poorly understood due to the lack of longitudinal observations of individual cells within the injured brain. After inducing focal demyelination with lysophosphatidylcholin (LPC) in the CC of adult mice, we used 2-photon microscopy to follow for up to 2...

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Dominik Olszewski
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Quantification of within patient Staphylococcus aureus phenotypic heterogeneity as a proxy for presence of persisters across clinical presentations

Julian Baer
Staphylococcus aureus was isolated from patients suffering cardiovascular, prosthetic joint or other infections and plated on Columbia sheep blood agar. Macroscopic time-lapse imaging was performed to quantify colony appearance-time and growth-rate. As a baseline reference, the same time-lapse imaging protocol was used after subculturing clinical isolates in tryptic soy broth until bacteria reached exponential growth phase.

Imaging mass cytometry and cell masks for 483 breast tumour samples from the METABRIC study

Hamid Ali
Multiplexed imaging of breast cancer tissues for 37 proteins

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Dominik Olszewski
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

High content genome-wide siRNA screen to investigate the coordination of cell size and RNA production

Micha Müller
Coordination of RNA abundance and transcription rates with cell size has been observed in diverse organisms and isogenic cell populations. However, how cells achieve such ‘size-scaling’ of transcription is unknown. Here we describe a genome-wide siRNA screen to find novel regulators of global RNA production in human cells. We quantify single-cell RNA production rates using metabolic pulse-labelling of RNA and subsequent high-content imaging. Our quantitative, single-cell measurements of DNA, nascent RNA, proliferating cell nuclear antigen...

Quantification of within patient Staphylococcus aureus phenotypic heterogeneity as a proxy for presence of persisters across clinical presentations

Julian Baer
Staphylococcus aureus was isolated from patients suffering cardiovascular, prosthetic joint or other infections and plated on Columbia sheep blood agar. Macroscopic time-lapse imaging was performed to quantify colony appearance-time and growth-rate. As a baseline reference, the same time-lapse imaging protocol was used after subculturing clinical isolates in tryptic soy broth until bacteria reached exponential growth phase.

Registration Year

  • 2022
    4
  • 2021
    2
  • 2020
    1

Resource Types

  • Image
    7

Affiliations

  • University of Zurich
    7
  • University Hospital of Zurich
    1
  • Cambridge Hospital
    1
  • University of British Columbia
    1
  • Cancer Research UK Cambridge Institute
    1