2 Works

3D super-resolution fluorescence microscopy maps the variable molecular architecture of the Nuclear Pore Complex

Jan Ellenberg
We explored the power of 3D super-resolution microscopy combined with computational classification and averaging to explore the 3D structure of the NPC in single human cells. We show that this approach can build the first integrated 3D structural map containing both central as well as peripheral NPC subunits with molecular specificity and nanoscale resolution. Our unbiased classification of over ten thousand individual NPCs indicates that the nuclear ring and the nuclear basket can adopt different...

Diffusion-mediated HEI10 coarsening can explain meiotic crossover positioning in Arabidopsis

Christopher Morgan
In most organisms, the number and distribution of crossovers that occur during meiosis are tightly controlled. All chromosomes must receive at least one “obligatory crossover” and crossovers are prevented from occurring near one another by “crossover interference”. However, the mechanistic basis of this phenomenon of crossover interference has remained mostly mysterious. Using quantitative super-resolution cytogenetics and mathematical modelling, we investigated crossover positioning in the Arabidopsis thaliana wild-type, an over-expressor of the conserved E3 ligase HEI10,...

Registration Year

  • 2021

Resource Types

  • Image


  • European Molecular Biology Laboratory
  • Max Delbrück Center for Molecular Medicine
  • John Innes Centre
  • ETH Zurich