Recent evidence suggests that groups of cells are more likely to form clinically dangerous metastatic tumors, emphasizing the importance of understanding mechanisms underlying collective behavior. The emergent collective behavior of migrating cell sheets in vitro has been shown to be disrupted in tumorigenic cells but the connection between this behavior and in vivo tumorigenicity remains unclear. We use particle image velocimetry to measure a multidimensional migration phenotype for genetically defined human breast epithelial cell lines...

Dataset of 2718 raw images taken for the study of transfer and persistence of UV powder deposited on a variety of clothing material. This dataset constitutes a ground truth for use as a model for forensic trace evidence.

Lightsheet microscopy has been previously used to image and directly study the process of gastrulation in a zebrafish embryo. Before now it was not possible to use this method to study late-stage somatogenesis, due to the movement of the embryo, resulting in displacement from the field of view. Here we achieve 4D lightsheet imaging with online, image based registration to maintain the field of view during long timelapses, with emphasis on the tailbud. The zebrafish...

Recent evidence suggests that groups of cells are more likely to form clinically dangerous metastatic tumors, emphasizing the importance of understanding mechanisms underlying collective behavior. The emergent collective behavior of migrating cell sheets in vitro has been shown to be disrupted in tumorigenic cells but the connection between this behavior and in vivo tumorigenicity remains unclear. We use particle image velocimetry to measure a multidimensional migration phenotype for genetically defined human breast epithelial cell lines...

We explored the power of 3D super-resolution microscopy combined with computational classification and averaging to explore the 3D structure of the NPC in single human cells. We show that this approach can build the first integrated 3D structural map containing both central as well as peripheral NPC subunits with molecular specificity and nanoscale resolution. Our unbiased classification of over ten thousand individual NPCs indicates that the nuclear ring and the nuclear basket can adopt different...

Proper spindle positioning is crucial for spatial cell division control. Spindle positioning in human cells relies on a ternary complex comprising Gαi1-3, LGN and NuMA, which anchors dynein at the cell cortex, thus enabling pulling forces to be exerted on astral microtubules. We developed a live imaging siRNA-based screen using stereotyped fibronectin micropatterns to uncover components modulating spindle positioning in human cells, testing 1280 genes, including all kinases and phosphatases. We thus discover 16 components...

Vegetation is a potential indicator of land contaminated by hydrocarbons. Remote sensing holds great potential for detecting stress in vegetation caused by the presence of hydrocarbons, but as yet this potential has not been realised, largely due to a lack of understanding of the biophysiological impacts of hydrocarbons on plant growth, and the specific spectral expression of them. Previous research has demonstrated success in laboratory simulation of hydrocarbon contamination and the analysis of the spectral...

Gene expression studies in early human development from 3 to 19 post conceptional weeks (PCW).

The coordination of cell movements across spatio-temporal scales ensures precise positioning of organs during vertebrate gastrulation. Mechanisms governing such morphogenetic movements have been studied only within a local region, a single germlayer or in whole embryos without cell identity. Scale-bridging imaging and automated analysis of cell dynamics are needed for a deeper understanding of tissue formation during gastrulation. Here we report pan-embryo analyses of formation and dynamics of all three germlayers simultaneously within a developing...

Away from the Mouse H&E quantification, the current version of MarrowQuant was tested on a small set of H&E bone marrow biopsies from anonymised patients. The tool performs in a comparable way as compared to the gold standard (Pathologists’ scorings), but it needs many optimisations before validating at the level of the human bone marrow biopsies quantifications and in clinical context.

We provide the first and complete atlas for morphometric analysis and visualization of heart tube morphogenesis, reporting morphological variability and early emergence of left-right asymmetry patterns.

LGBT+ people are evidenced globally as being negatively impacted by the criminal justice system and face specific challenges within custodial settings due to their sexual orientation or gender identity, in what are inherently heteronormative and overtly masculine spaces culturally, and where hard lines between sex and gender are actively enforced and even reified in policy and practice at the institutional level. This dissertation sought to explore the experience of LGBT+ people in UK prisons relative...

Data collected from the centrifuge testing programme of six screw piles for the Supergen Wind Hub Grand Challenges

This dataset contains data of experiments underlying the work presented in the linked Ecological Engineering journal publication (DOI: 10.1016/j.ecoleng.2022.106621). Source code for the DRAM model (written in R) can be found in a separate repository (DOI: 10.5281/zenodo.6375587).

Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

The immunological synapse is a complex structure that decodes stimulatory signals into adapted lymphocyte responses. As such, it represents a unique window to monitor lymphocyte activity, owing to the development of systematic quantitative approaches applicable to primary cells. Here we demonstrate the applicability of high-content imaging to human T and NK cells and develop a pipeline for unbiased analysis of high-definition morphological profiles. Our approach reveals how distinct facets of actin cytoskeleton remodeling shape immunological...

Poster related to a study exploring the ongoing multiple and multi-dimensional transitions experienced by medicine, nursing and dentistry students due to graduate in summer 2020.

Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Community development is commonly described as contested practice, it is defined in many nuanced ways and there are challenges across the literature encouraging practitioners to engage more fully with critical thinking and transformative intent. The aim of this inquiry was to investigate community development practitioners’ perspectives on their practice in Scotland. Using an interpretivist framework, the research was designed to foreground the participants’ voices as experts on their own practice. Dyadic dialogical interviews were developed...

A qualitative case study approach was adopted, focusing on the development of shale gas in specific licensed areas. Semi structured interviews and documentary analysis were used to obtain the perspectives of engagement managers, regulators, local communities, Non-Governmental Organisations (NGOs) and industry experts. Collectively, all study participants were stakeholders in the shale gas development process. There was consistency in the understanding of who or what constituted as stakeholders; however, stakeholder engagement was often viewed as prescriptive,...

The goal of this study is to present a methodology to create 3 dimensional structures reconstructed from high resolution histological sections by utilizing convolutional neural networks to annotate the images, discard the folded and/or torn section with an unsupervised information-theory-based clustering algorithm and apply Homography to correct perspective of each image. The resulting models then can be used for 3 dimensional micromorphometry analysis to aid the decision making process of medical professionals.

Important questions about the development of a common European energy security policy have been unexplored in the literature. The literature on European energy security focuses on the EU’s internal and external energy policy and explores issues ranging from the importance of speaking with one voice to who is leading European energy policy. Other studies focus on how much progress has been made towards the development of a common European energy security policy, what factors have...

This dataset consists of economic implied sentiments based on filtering of articles based on economic policy keywords. The process of filtering is described in : Rambaccussing, D., & Kwiatkowski, A. (2020). Forecasting with news sentiment: Evidence with UK newspapers. International Journal of Forecasting, 36(4), 1501-1516. Bowden, J., Kwiatkowski, A., & Rambaccussing, D. (2019). Economy through a lens: distortions of policy coverage in UK national newspapers. Journal of Comparative Economics, 47(4), 881-906. The construction of the...

Background The nursing profession has been challenged to improve standards of care in the UK. Nursing has been, and continues to be, considered a caring profession, but this reputation has been undermined since the events in Mid-Staffordshire Hospitals emerged between 2005 and 2009. The ensuing debate in the professional nursing journals sought to apportion blame under the guise of seeking solutions. Many arguments were revisited. This included scrutiny of undergraduate nursing education post-1996 when provision...