Systematic reviews (e.g. Sandall et al. 2015) have demonstrated a link between midwifery-led continuity of care models and improved Clinical, Psychosocial and Organisational (CPO) outcomes, including preterm birth and breastfeeding rates. However, the underlying causal mechanisms within these models are not understood, and trial reports rarely detail all the relevant contextual factors for the intervention and control groups. This pilot study will test the use of focus groups with service users and providers across NHS...

Tissue clearing is one of the most powerful strategies for a comprehensive analysis of disease progression. Here, we established an integrated pipeline that combines tissue clearing, 3D imaging, and machine learning and applied to a mouse tumour model of experimental lung metastasis using human lung adenocarcinoma A549 cells. This pipeline provided the spatial information of the tumour microenvironment. We further explored the role of transforming growth factor-beta (TGF-beta) in cancer metastasis. TGF-beta-stimulated cancer cells enhanced...

Petrol samples were collected directly from nine different public fuelling stations in Glasgow, Scotland. Samples of 10, 25, 50, 75, 90, and 95% evaporated product were prepared, resulting in 63 samples in total which were analyzed using gas chromatography–mass spectrometry.

Media content to archive for the Nunalleq Educational Resource project (2017-2019). This contains an organised folder structure containing 3D model files, audio files, video files as well as text and photoshop files outlining the structure of the original resource. Additionally we have provided a 'walkthrough' video which demonstrated how the interactions work within the resource. The installers for the current resource build which were distributed to schools in Alaska and made available for public download...

0.177 calibre pellets from a newly purchased air pistol were fired in a series and were examined using an optical microscope.

Oligodendrocyte precursor cells (OPCs) retain the capacity to remyelinate axons in the corpus callosum (CC) upon demyelination. However, the dynamics of OPC activation, mode of cell division, migration, and differentiation on a single cell level remain poorly understood due to the lack of longitudinal observations of individual cells within the injured brain. After inducing focal demyelination with lysophosphatidylcholin (LPC) in the CC of adult mice, we used 2-photon microscopy to follow for up to 2...

In most organisms, the number and distribution of crossovers that occur during meiosis are tightly controlled. All chromosomes must receive at least one “obligatory crossover” and crossovers are prevented from occurring near one another by “crossover interference”. However, the mechanistic basis of this phenomenon of crossover interference has remained mostly mysterious. Using quantitative super-resolution cytogenetics and mathematical modelling, we investigated crossover positioning in the Arabidopsis thaliana wild-type, an over-expressor of the conserved E3 ligase HEI10,...

The Leverhulme Research Centre for forensic science (LRCFS) at the University of Dundee has had several ongoing projects (collectively known by the project name Bruin) in the area of digital documentation of simulated crime scenes, particularly focusing on the 'art of the possible' using Virtual Reality as technology tools for facilitating this. LRCFS and NKC always welcome collaborations and look forward to positive discussions on how we can work together in partnership in this emerging...

During early Drosophila embryogenesis, a network of gene regulatory interactions orchestrates terminal patterning, playing a critical role in the subsequent formation of the gut. We utilized CRISPR gene editing at endogenous loci to create live reporters of transcription and light-sheet microscopy to monitor the individual components of posterior patterning network across 90 minutes prior to gastrulation. We developed a computational pipeline for fusing imaging datasets of the individual components into a common multivariable trajectory. This...

The aim of the study was to investigate relationships between ALS-related RNA-binding proteins and ALS, under different stress conditions.

We report the development of a semi-automated image analysis plug-in, MarrowQuant, which employs the open-source software QuPath, to systematically quantify multiple bone components in H&E sections in an unbiased manner. MarrowQuant discerns and quantifies the areas occupied by bone, adipocyte ghosts, hematopoietic cells and the interstitial/microvascular compartment. A separate feature, AdipoQuant, fragments adipocyte ghosts in H&E-stained sections of extramedullary adipose tissue sections to render adipocyte area and size distribution. Quantification of BM hematopoietic cellularity with...

This dataset contains all data underlying the work presented in the linked Geotechnique journal publication. It contains the result of all experimental work described, as well as all numerical code (mixture of MATLAB, Python and R code) and numerical predictions for all computational models described.

Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Sun (2018) develops a narrative index to comprehensively measure the PBC's policy stance (Sun-MP index), based on the additional information on policy-makers’ intentions extracted from the PBC's documents. It is a five-value index on a quarterly basis. The policy stance of -2 stands for strong easing, indicating a strong emphasis on promoting real growth; 2 for strong tightening that indicates a strong policy emphasis on inflation reduction; -1 for easing and 1 for tightening, indicating...

There seems a natural connection between human bodies, animal bodies, and books, not least because they are all containers, vessels of knowledge and experience. So I explored this year many materials that have skin-like qualities. And I began to think about how fabrics are like a second skin, worn to cover the human skin underneath. And I investigated how these exploratory materials might become something like prosthetics, or barriers and protection from anything touching the...

The immunological synapse is a complex structure that decodes stimulatory signals into adapted lymphocyte responses. As such, it represents a unique window to monitor lymphocyte activity, owing to the development of systematic quantitative approaches applicable to primary cells. Here we demonstrate the applicability of high-content imaging to human T and NK cells and develop a pipeline for unbiased analysis of high-definition morphological profiles. Our approach reveals how distinct facets of actin cytoskeleton remodeling shape immunological...

The immunological synapse is a complex structure that decodes stimulatory signals into adapted lymphocyte responses. As such, it represents a unique window to monitor lymphocyte activity, owing to the development of systematic quantitative approaches applicable to primary cells. Here we demonstrate the applicability of high-content imaging to human T and NK cells and develop a pipeline for unbiased analysis of high-definition morphological profiles. Our approach reveals how distinct facets of actin cytoskeleton remodeling shape immunological...

The immunological synapse is a complex structure that decodes stimulatory signals into adapted lymphocyte responses. As such, it represents a unique window to monitor lymphocyte activity, owing to the development of systematic quantitative approaches applicable to primary cells. Here we demonstrate the applicability of high-content imaging to human T and NK cells and develop a pipeline for unbiased analysis of high-definition morphological profiles. Our approach reveals how distinct facets of actin cytoskeleton remodeling shape immunological...

Long acting beta agonists (LABA) and long acting muscarinic receptor antagonists (LAMA) are both used in the treatment of obstructive airway disease, in asthma they are given with inhaled corticosteroids (ICS), in chronic obstructive pulmonary disease (COPD) they may be given with or without ICS. These therapies may attenuate the degree to which airways narrow to a bronchoconstrictor stimulus (airway hyperresponsiveness). Long acting bronchodilators may also change airway geometry as well as pure airway calibre,...

Bronchiectasis is a pulmonary condition defined by abnormal bronchial dilation resulting in chronic cough, inflammation, and repeated chest infections. Cilia are tiny hair-like structures in the airways which beat in a co-ordinated fashion to remove harmful pathogens trapped by mucus. Mucociliary dysfunction is a feature of inflammatory lung diseases like bronchiectasis and COPD which are believed to be primarily Th1 driven, and asthma which is believed to be predominantly Th2 driven. It is now recognised...

We consider the mathematical modelling of smectic A liquid crystals using the continuum dynamic theory for smectic A liquid crystals that has been developed by Stewart [79]. We also examine biaxial nematic liquid crystals using the continuum theory of Leslie and co-workers [50,51] and the review by [80]. The Helfrich-Hurault transition is considered in this thesis to study the influence of the compression coefficient B0 when an electric field is applied. The molecular alignment described...