29 Works

The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue

Fabian F. Voigt
Comparison of whole-mouse brain data acquired from a CLARITY-cleared VIP-tdTomato mouse with and without ASLM mode engaged

The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue

Fabian F. Voigt
Imaging a chicken embryo with the mesoSPIM

Imaging Mass Cytometry of human tonsil sections

Segura
We analysed the in situ localisation of immune cell types in human tonsils using imaging mass cytometry.

Analysis of coupled axial and lateral deformation of roots in soil — figure data

Meijer, Muir Wood, Knappett, Bengough & Liang Teng
This data archive is linked to the following publication: ‘Analysis of coupled axial and lateral deformation of roots in soil’ by G.J. Meijer, D. Muir Wood, J.A. Knappett, A.G. Bengough and T. Liang, as published in the International Journal for Analytical and Numerical Methods in Geomechanics (https://doi.org/10.1002/nag.2880). This data achieve contains all data required to replot any graph in this manuscript (Figures 8-18 and 20-22). Note that this paper is covered by a CC-BY license,...

Chemical composition of different sources of diesel fuel

Nic Daeid & Mat-Desa
Diesel samples were collected directly from eight different public fuelling stations in Glasgow, Scotland. Samples of 10, 25, 50, 75, 90, and 95% evaporated product were prepared, resulting in 56 samples in total which were analyzed using gas chromatography–mass spectrometry.

Audience and Expert Perspectives on Second Screens

Gorkovenko & Taylor
This dataset contains transcripts of co-design workshops and expert interviews carried out as part of a research project exploring how second screens (i.e. devices used while watching television) could be used to support engagement with televised political debates. We conducted four two-hour workshops with 18 participants in total, where they discussed the issues with political discourse online at large and the opportunities for second screens. The discussions were structured around four open-ended topics: (1) content,...

Uncovering the signaling landscape controlling breast cancer cell migration identifies novel metastasis driver genes

Esmee Koedoot
Triple-negative breast cancer (TNBC) is an aggressive and highly metastatic breast cancer subtype. Enhanced TNBC cell motility is a prerequisite of TNBC cell dissemination. Here, we apply an imaging-based RNAi phenotypic cell migration screen using two highly motile TNBC cell lines (Hs578T and MDA-MB-231) to provide a repository of signaling determinants that functionally drive TNBC cell motility.

Uncovering the signaling landscape controlling breast cancer cell migration identifies novel metastasis driver genes

Esmee Koedoot
Primary RNAi screen using 4198 smartpool siRNAs to identify cell migratory regulators in Hs578T and MDA-MB-231 triple-negative breast cancer cells.

Identifying Extrinsic versus Intrinsic Drivers of Variation in Cell Behavior in Human iPSC Lines from Healthy Donors

Vigilante, Laddach, Moens, Meleckyte, Leha, Ghahramani, Culley, Kathuria, Hurling, Vickers, Wiseman, Tewary, Zandstra, , Durbin, Fraternali, Stegle, Birney, Luscombe, Danovi & Watt
Large cohorts of human induced pluripotent stem cells (iPSCs) from healthy donors are a potentially powerful tool for investigating the relationship between genetic variants and cellular behavior. Here, we integrate high content imaging of cell shape, proliferation, and other phenotypes with gene expression and DNA sequence datasets from over 100 human iPSC lines. By applying a dimensionality reduction approach, Probabilistic Estimation of Expression Residuals (PEER), we extracted factors that captured the effects of intrinsic (genetic...

Air Pistol Pellet Dataset

Hamzah & Nic Daeid
Projectiles from a newly purchased air pistol with 0.177 calibre pellets (unjacketed) were fired a series of pellets and were examined using an optical microscope.

A quantitative map of human Condensins provides new insights into mitotic chromosome architecture

Jan Ellenberg
Homozygous genome editing was used to fluorescently tag subunits of Condensin I and II in HeLa cells. Absolute abundance, spacing, and dynamic localization of the tagged proteins during mitosis was recorded by fluorescence correlation spectroscopy (FSC)-calibrated live-cell imaging and superresolution microscopy.

The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue

Fabian F. Voigt
The mesoscale selective plane illumination microscope (mesoSPIM) designed in this study was tested with various cleared tissue samples.

Mapping the perturbome network of cellular perturbations

Jörg Menche

Meiotic cellular rejuvenation is coupled to nuclear remodeling in budding yeast

Elçin Ünal

Chemical composition of different sources of petrol

Nic Daeid & Mat-Desa
Petrol samples were collected directly from nine different public fuelling stations in Glasgow, Scotland. Samples of 10, 25, 50, 75, 90, and 95% evaporated product were prepared, resulting in 63 samples in total which were analyzed using gas chromatography–mass spectrometry.

Examination of rifling lands on fired air pellets

Hamzah & Nic Daeid
0.177 calibre pellets from a newly purchased air pistol were fired in a series and were examined using an optical microscope.

The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue

Fabian F. Voigt
Imaging a whole mouse central nervous system with the mesoSPIM

A quantitative map of human Condensins provides new insights into mitotic chromosome architecture

Jan Ellenberg
FRAP experiments of Condensin-mEGFP subunits on the metaphase plate.

A quantitative map of human Condensins provides new insights into mitotic chromosome architecture

Jan Ellenberg
Single Colour - Condensin subunits were immunostained (with an anti-GFP antibody), and mitotic cells with chromatids oriented in parallel to the focal plane were selected for imaging. DNA (Hoechst) and Condensins (mEGFP tag and anti-GFP immunostaining) were imaged by diffraction-limited microscopy, whereas immunostained Condensin was also imaged by STED microscopy. Double Colour - NCAPH2-mEGFP and NCAPH-Halo were immunostained respectively with anti-GFP (1:500; ab13970; Abcam) + goat secondary anti-chicken Alexa Fluor 594 (1;500; A-11042; Thermo Fisher...

Live imaging screen reveals that TYRO3 and GAK ensure accurate spindle positioning in human cells

Benita Wolf, Coralie Busso & Pierre Gönczy
Proper spindle positioning is crucial for spatial cell division control. Spindle positioning in human cells relies on a ternary complex comprising Gαi1-3, LGN and NuMA, which anchors dynein at the cell cortex, thus enabling pulling forces to be exerted on astral microtubules. We developed a live imaging siRNA-based screen using stereotyped fibronectin micropatterns to uncover components modulating spindle positioning in human cells, testing 1280 genes, including all kinases and phosphatases. We thus discover 16 components...

Consent and Inclusion of care home residents: Additional research materials, analysis and results

Marianne Dee & Vicki Hanson
This site contains information on the gathering of data (2016-17) from the residents of care homes for older people in the U.K. The study created a novel visual game activity to enhance engagement with residents, and a redesigned ethical consenting approach to be more accessible, equitable and inclusive. Described are the tools used to develop a visual method of interacting with vulnerable people to better facilitate interviews with them. This includes the research materials, the...

The mesoSPIM initiative – open-source light-sheet microscopes for imaging cleared tissue

Fabian F. Voigt
Imaging a whole mouse central nervous system with the mesoSPIM

Chemical composition of different light petroleum distillates (LPD)

Nic Daeid & Mat-Desa
Lighter fuel samples from five different commercial sources were purchased. Samples of 10, 25, 50, 75, 90, and 95% evaporated product were prepared, resulting in 40 samples in total which were analyzed using gas chromatography–mass spectrometry.

Analysis of cyclic straining on cytoskeletal systems

Merkel
Characterization of cytoskeletal systems (actin, microtubules, vimentin networks) to cyclic straining using confocal laser scanning microscopy, including experiments with nocodazole to depolymerize microtubules.

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