51 Works

Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH

Magda Bienko
We describe a dataset obtained by applying our recently developed iFISH pipeline to simultaneously visualize 16 small (size range: 62–73 kilobases, kb) evenly spaced DNA loci on chromosome 2 in human cells, in a single round of hybridization. We show how, using combinatorial color coding, multiple loci can be precisely 3D localized in single cells, and how inter-loci distances scale inversely with chromosome contact frequencies determined by Hi-C, as expected. We provide raw images and...

Quantification of within patient Staphylococcus aureus phenotypic heterogeneity as a proxy for presence of persisters across clinical presentations

Julian Baer
Staphylococcus aureus was isolated from patients suffering cardiovascular, prosthetic joint or other infections and plated on Columbia sheep blood agar. Macroscopic time-lapse imaging was performed to quantify colony appearance-time and growth-rate. As a baseline reference, the same time-lapse imaging protocol was used after subculturing clinical isolates in tryptic soy broth until bacteria reached exponential growth phase.

Quantification of within patient Staphylococcus aureus phenotypic heterogeneity as a proxy for presence of persisters across clinical presentations

Julian Baer
Staphylococcus aureus was isolated from patients suffering cardiovascular, prosthetic joint or other infections and plated on Columbia sheep blood agar. Macroscopic time-lapse imaging was performed to quantify colony appearance-time and growth-rate. As a baseline reference, the same time-lapse imaging protocol was used after subculturing clinical isolates in tryptic soy broth until bacteria reached exponential growth phase.

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Fanny Georgi
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

A Ground Truth Data Set of Gas Chromatography mass spectrometry (GCMS) Analysed Synthesised Methylenedioxymethylamphetamine (MDMA)

Jonathan Miller, Niamh NicDaeid & Roberto Puch-Solis
An open source format data set of Methylenedioxymethylamphetamine. This data set was derived from a PhD project which involved the repetitive chemical synthesis of a series of methylenedioxymethylamphetamine (MDMA) samples following a series of known synthetic chemical routes. Controlled drug samples are normally chemically analysed to determine their identity and in some cases, their purity. There are also circumstances where a more broad chemical characterisation of drug samples may also be required. This involves investigating...

Individual and Work Environment Factors that Influence Nursing Faculties’ Well-being, Engagement and Intention to Remain

Alya Alghamdi
Background: Saudi Arabian higher education institutions are facing major problems in retaining nursing faculty members. The reasons for this are unclear and there is limited literature specific to the Middle East and Saudi Arabia. Methods/Design: This current study adopted a mixed methods approach (sequential, explanatory) to understand the factors that contribute to the retention of nursing staff in higher education institutions in Saudi Arabia. This research was carried out in four phases–two scoping and a...

Quantification of within patient Staphylococcus aureus phenotypic heterogeneity as a proxy for presence of persisters across clinical presentations

Julian Baer
Staphylococcus aureus was isolated from patients suffering cardiovascular, prosthetic joint or other infections and plated on Columbia sheep blood agar. Macroscopic time-lapse imaging was performed to quantify colony appearance-time and growth-rate. As a baseline reference, the same time-lapse imaging protocol was used after subculturing clinical isolates in tryptic soy broth until bacteria reached exponential growth phase.

Quantification of within patient Staphylococcus aureus phenotypic heterogeneity as a proxy for presence of persisters across clinical presentations

Julian Baer
Staphylococcus aureus was isolated from patients suffering cardiovascular, prosthetic joint or other infections and plated on Columbia sheep blood agar. Macroscopic time-lapse imaging was performed to quantify colony appearance-time and growth-rate. As a baseline reference, the same time-lapse imaging protocol was used after subculturing clinical isolates in tryptic soy broth until bacteria reached exponential growth phase.

Visual Narration in Comics and Graphic Novels

Yufei Gong
The past year I have investigated storyboards and the processes of visual narration. I have also looked at illustration, and the first experiment in photo-etching. I see visual narration as influenced by the camera's framing, and through the use of the imaginary camera, I have drawn storyboards and made many pages of compositions.

Seven tips for practitioners

Camila Biazus Dalcin, Andrea Rodriguez, Fernando Fernandes, Ann Swinney & Sally Haw
This information is for practitioners and healthcare professionals who work with young people leaving custody. Here are some tips for accessing different healthcare services in Scotland.

Stepwise-edited, human melanoma models reveal mutations’ effect on tumor and microenvironment

Eran Hodis & Elena Torlai Triglia
Establishing causal relationships between genetic alterations of human cancers and the specific phenotypes of malignancy remains a challenge. We sequentially introduced mutations into healthy human melanocytes in up to five genes spanning six commonly disrupted melanoma pathways forming nine genetically distinct cellular models of melanoma. We connected mutant melanocyte genotypes to malignant cell expression programs in vitro and in vivo, replicative immortality, malignancy, rapid tumor growth, pigmentation, metastasis, and histopathology. Mutations in malignant cells also...

A quantitative map of nuclear pore assembly reveals two distinct mechanisms

Shotaro Otsuka & Jan Ellenberg
3D time-lapse imaging of nucleoporins

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Fanny Georgi
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Dominik Olszewski
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Fanny Georgi
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Fanny Georgi
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

A Bliss Writing System

Palatuwa Nandadasa
Being able to communicate in the natural environment and sufficiently meet daily communication needs is the essence of communication. The majority of the population in the world does not think about the mechanics required to communicate effectively. However, there is a significant number of people who experience difficulties in communication due to speech and/or cognitive impairments. Alternative methods of communication can be used to help people with disabilities to maximise the competence of communication. These...

Reference BioImaging dataset to assess the phenotypic trait diversity of bryophytes within the family Scapaniaceae

Kristian Peters
Here, we present a high-quality reference dataset containing macroscopic and bright-field microscopic images documenting various phenotypic properties of the species belonging to the family of Scapaniaceae occurring in Europe. The information contained in the images is of particular interest for biodiversity research, machine learning and image segmentation used in bioinformatics and computational ecology.

Time Series of Media Implied Economic Sentiments (Dictionary)

Dooruj Rambaccussing & Andrzej Kwiatkowski
These are monthly time series of media implied economic sentiments based on economic policy coverage in the main Broadsheet Newspapers in United Kingom. The time series was derived using unsupervised Machine learning where assignment of sentiment scores was achieved using the Loughran-MacDonald Dictionary for assignment of positive and negative scores.

Designing Appropriately for Optimum Environments to Care for Dementia/Alzheimer’s Residents

Ziyue Tao
According to the World Health Organization, dementia is now the fifth largest killer in the world, although dementia is the number one killer in some wealthier countries. Globally, 50 million people suffer from this disease, but due to an aging population, this number is expected to increase to 130 by 2050 million. The 2020 dementia study on Chinese people over 60 years old shows that there are approximately 15.07 million people with dementia among Chinese...

Relationships Between Skin and Fabric, with attention to experimental materials recreating skin-like qualities

Yingying Ren
There seems a natural connection between human bodies, animal bodies, and books, not least because they are all containers, vessels of knowledge and experience. So I explored this year many materials that have skin-like qualities. And I began to think about how fabrics are like a second skin, worn to cover the human skin underneath. And I investigated how these exploratory materials might become something like prosthetics, or barriers and protection from anything touching the...

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Dominik Olszewski
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Fanny Georgi
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Fanny Georgi
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Parallel compound screen for infection modulators identifies re-purposing candidates against common viral infections.

Dominik Olszewski
Viral infectious diseases span a myriad of maladies, including immunosuppression, cancer, organ failure and, if lacking effective treatment, even death. While numerous targeted antiviral therapies have been developed over the past century, many viral diseases still lack effective antivirals to date. Revisiting compounds approved for other applications offers accelerated development, and automated screening and data processing boost the feasibility of the re-purposing approach. Here, we extended our high-content image-based platform on three human viruses differing...

Registration Year

  • 2022
    51

Resource Types

  • Image
    30
  • Dataset
    9
  • Text
    9
  • Other
    2
  • Audiovisual
    1

Affiliations

  • University of Dundee
    20
  • Paul Sabatier University
    5
  • University of Zurich
    4
  • Harvard University
    2
  • Max F. Perutz Laboratories
    2
  • University of Bath
    1
  • Henan University
    1
  • Zhejiang University
    1
  • European Molecular Biology Laboratory
    1
  • University Hospital of Zurich
    1