Cardiac histopathology from human patients with clinical heart failure or cadaveric donor hearts from patients without clinical heart failure. Human tissue research: Human heart tissue was procured from two separate groups of subjects: heart transplant or LVAD recipients with severe heart failure (Fal), and brain dead, organ donors with no history of heart failure (non-failing, NF). Tissue from patients with ischemic cardiomyopathy sampled infarct-free regions. No organs or tissue were procured from prisoners. Prospective informed...

Image data used to build the mitotic cell atlas. This consists of three subsets: raw images, segmentation masks for the landmarks and protein concentration maps.

The mouse embryo has long been central to the study of mammalian development; however, elucidating the cell behaviors governing gastrulation and the formation of tissues and organs remains a fundamental challenge. A major obstacle is the lack of live imaging and image analysis technologies capable of systematically following cellular dynamics across the developing embryo. We developed a light-sheet microscope that adapts itself to the dramatic changes in size, shape and optical properties of the post-implantation...

At the beginning of mammalian life the genetic material from each parent meets when the fertilized egg divides. It was previously thought that a single microtubule spindle is responsible to spatially combine the two genomes and then segregate them to create the two-cell embryo. Utilizing light-sheet microscopy, we showed that two bipolar spindles form in the zygote, which independently congress the maternal and paternal genomes. These two spindles aligned their poles prior to anaphase but...

During development, morphogens provide extracellular cues allowing cells to select a specific fate by inducing complex transcriptional programs. The mating pathway in budding yeast offers simplified settings to understand this process. Pheromone secreted by the mating partner triggers the activity of a MAPK pathway, which results in the expression of hundreds of genes. Using a dynamic expression reporter, we quantified the kinetics of gene expression in single cells upon exogenous pheromone stimulation and in the...

Confocal microscopy images: All of the samples after shear stress treatment were observed using Leica confocal microscopy. The images show fields of view under microscopy. Excel chart for data analysis: Bacterial counting was performed by the software Image J, and analysed using Excel.

Lightsheet microscopy has been previously used to image and directly study the process of gastrulation in a zebrafish embryo. Before now it was not possible to use this method to study late-stage somatogenesis, due to the movement of the embryo, resulting in displacement from the field of view. Here we achieve 4D lightsheet imaging with online, image based registration to maintain the field of view during long timelapses, with emphasis on the tailbud. The zebrafish...