Immunoassays and mass spectrometry are powerful single-cell protein analysis tools; however, interfacing and throughput bottlenecks remain. Here, we introduce three-dimensional single-cell immunoblots to detect both cytosolic and nuclear proteins. The 3D microfluidic device is a photoactive polyacrylamide gel with a microwell array-patterned face (x-y) for cell isolation and lysis. Single-cell lysate in each microwell is ‘electrophoretically projected’ into the 3rd dimension (z-axis), separated by size, and photo-captured in the gel for immunoprobing and confocal/light-sheet imaging....
Handwritten notes accompanied by letters from Kroeber to Hans Uldall dated October 8 and 21, 1932. Formerly in the possession of Jesse Sawyer.
Targeted occupant surveys: A novel method to effectively relate occupant feedback with environmental conditionsCarlos Duarte Roa, Stefano Schiavon & Thomas Parkinson
Occupant satisfaction surveys are widely used in laboratory and field research studies of indoor environmental quality. Field studies pose several challenges because researchers usually have no control over the indoor environments experienced by building occupants, it is difficult to recruit and retain participants, and data collection methods can be cumbersome. With this in mind, we developed a survey platform that uses real-time feedback to send targeted occupant surveys (TOS) at specific indoor environmental conditions and...
Typed class paper, with instructor handwritten comments.
Photocopy of typed class paper, with instructor handwritten comments (in photocopy).
Typed final co-authored class paper.
Typed class paper.
Photocopy of typed final class paper.
Handwritten transcription with glosses and free translation. See related file bundle for original sound recording.
Typed transcription with glosses and free translation, with handwritten annotations by instructor. See related file bundle for original sound recording.
Datasets supporting the manuscript, "Particle-in-cell modeling of Martian magnetic cusps and their role in enhancing nightside ionospheric ion escape", published in Geophysical Research Letters. All datasets are output from a 1.5-dimensional, particle-in-cell model of magnetospheric-ionospheric particle interactions along an open magnetic field line in a Martian magnetic cusp region. Particles are self-consistently traced according to the combined magnetic mirror force, parallel electrostatic fields, and Martian gravity. Particle densities, velocity distributions, and domain potentials are printed...
Audio file: VP-060618-08: Goddard verb paradigm check; ‘he is catching’ (ch’ixa:wh), from Goddard 1905, pp. 96-97 ("Morphology of the Hupa Language," University of California Publications in American Archaeology and Ethnology 3).