1,455 Works

The Organ Care System as a new promising tool for donor heart ex vivo preservation

Antonella Bonetti, Giovanni Benedetti, Cristina Cantarutti, Veronica Ferrara, Magali Contin, Nicoletta Finato, Sandro Sponga, Gennaro Esposito, Ugolino Livi & Fulvia Ortolani
Heart transplantation remains the gold standard treatment for end-stage heart failure. To face actual donor shortage, heart warm perfusion with the Organ Care System (OCS) was introduced alternatively to usual cold ischemic storage [1]. Here, OCS-preserved hearts were matched against those subjected to cold ischemia in terms of (i) perioperative clinical parameters, (ii) histopathological, immunohistochemical, and ultrastructural features of pre- and post-implant left ventricle biopsies, and (iii) cardiomyocyte metabolism by NMR spectroscopy of blood samples....

Effects of the endoplasmic reticulum signaling pathway on cadmium-induced impairment of the blood brain barrier

Jacopo J.V. Branca, Mario Maresca, Gabriele Morucci, Ferdinando Paternostro, Lorenzo Di Cesare Mannelli, Massimo Gulisano & Alessandra Pacini
Due to its high permeability to blood-brain barrier (BBB), cadmium (Cd) has been regarded as a possible etiological factor for human neurodegenerative diseases, such as Parkinson’s disease, Alzheimer’s disease, and Huntington’s disease [1]. However, the mechanism underlying cadmium-induced BBB permeability remains unclear. In this study, we investigated the effect of Cd in RBE4 cells (rat brain endothelial cells) and delineated the signaling pathway that, triggering endoplasmic reticulum (ER) stress and caspase 3 activation, leads to...

Does Pelvic Incidence Influence the Morphology of the Sacroiliac Joint?

Giorgio Cacciola, Federico De Meo, Angelo Favaloro, Alessandro Pisani, Piero Cavaliere, Andrea Barbanera, Elena Filippini, Carmelo Milazzo & Debora Di Mauro
Pelvic Incidence is defined as the angle between the perpendicular line to the upper plate of S1 at its midpoint and the line between this point and the center of bicoxofemoral line, it describes the position of femural heads in relation to sacrum. Recently some authors described a direct correlation between high values of PI and large AP pelvic axis (horizontal pelvis) and a wide pelvic ring [1]. Also the acetabular orientation is influenced by...

Judo training for older individuals with control group: An anthropometric evaluation

Simone Ciaccioni, Giancarlo Condello & Laura Capranica
The aim of this study was to investigate the effects of a 4-month judo training (1-hr training session, twice a week) on anthropometrical variables in older individuals (age: 60-76 yrs). The experimental group (JG) included 19 (F=9, M=10; 68.9±3.7yrs) participants to a 4-month judo programme, whereas the control group (CG) encompassed 14 (F=7, M=7; CG: 69.9±4.9 yrs) sedentary controls (CG). The considered anthropometric variables were: weight (Wt), height (Ht), body mass index (BMI), waist circumference...

The pink adipocytes

Saverio Cinti
Most of white and brown adipocytes, in spite of their well-known different functions: i.e. storing energy (white) and thermogenesis (brown), are contained together in visceral and subcutaneous depots (adipose organ) in all mammals including humans (1, 2). A growing body of evidence suggests that the reason for this anatomical mixture could reside in the fact that adipocytes have peculiar plastic properties allowing them to convert directly each other under appropriate stimuli (3). Under chronic cold...

Sarcoglycan sub-complex in the adipose organ: a molecular and immunofluorescence study

Saverio Cinti, Giuseppina Rizzo, Giovanna Vermiglio, Cecilia Spoto, Antonio Centofanti & Giuseppina Cutroneo
The sarcoglycan sub-complex (SGC) is made up of six glycoproteins which connect the cytoskeleton to the extracellular matrix in skeletal muscle. Recent data show that this complex is also expressed in epithelial tissue such as gingival, breast and prostatic ones [1]. The adipose organ is organized in multiple fat depots consisting of white and brown adipose cells. White adipocytes can store energy in triglycerides; brown adipocytes can dissipate energy for thermogenesis. It has been demonstrated...

Neuronal Antibodies and Brain alterations in APECED Patients

Giulia Corda, Antonella Meloni, Luca Saba, Stefano Mariotti, Gian-Luca Ferri & Cristina Cocco
APECED (Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Distrophy) is a rare autosomal recessive disorder. We previously found that sera samples from 9/14 patients revealed autoantibodies (Auto-Abs) reacting with cerebellum (GABAergic cells, n=5) and substantia nigra (SN; dopaminergic cells, n=5) [1]. Follow-up of the large majority of these patients was perfomed at least 10 years after the previous investigation. Indeed, on these patients, and on control age-matched subjects (n=14), we performed brain examinations using an MRI scanner. Obtained images were...

Cancer stem cells from peritumoral tissue of glioblastoma multiforme: the possible link between tumor development and progression

Alessio D’Alessio, Cristiana Angelucci, Elena Binda, Annunziato Mangiola, Gabriella Proietti, Laura Masuelli, Barbara Fazi, Silvia Anna Ciafrè & Gina Lama
In glioblastoma multiforme (GBM), cancer stem cells (CSCs) are thought to be responsible for gliomagenesis, resistance to treatment and recurrence. Unfortunately, the prognosis for GBM remains poor and recurrence frequently occurs in the peritumoral tissue within 2 cm from the tumor edge. In this area, a population of CSCs has been demonstrated which may recapitulate the tumor after surgical resection. In the present study, we aimed to characterize CSCs derived from both peritumoral tissue (PCSCs)...

Diffusion Tensor Imaging (DTI) in Head and Neck Oncology; a new method of Virtual Biopsy

Alessandro Calvo, Michele Runci Anastasi, Antonio Duca, Giuseppe Anastasi, Demetrio Milardi & Francesco Saverio De Ponte
The objective of our study is to explore the feasibility and utility of Diffusion Tensor Imaging (DTI) in the non invasive evaluation of any lesion [1] in Head and Neck structure. We would reach a verified and certified step of Virtual biopsy. Ten patients with different histological lesions were recruited for this study. Morphological and diffusion weighted images were acquired with a 3T scanner during last 3 months. Probabilistic tensor-based tractography reconstruction of any lesions...

Identification of an HSP90 modulated multi-step process for ERBB2 degradation in breast cancer cells

Patrizia Castagnola, Grazia Bellese, Filippo Birocchi, Maria Cristina Gagliani, Carlo Tacchetti & Katia Cortese
The receptor tyrosine kinase ERBB2 interacts with HSP90 and is overexpressed in aggressive breast cancers. Therapeutic HSP90 inhibitors, i.e. Geldanamycin (GA), target ERBB2 to degradation. We have previously shown that HSP90 is responsible for the missorting of recycling ERBB2 to degradation compartments. In this study, we used biochemical, immunofluorescence and electron microscopy techniques to demonstrate that in SKBR3 human breast cancer cells, GA strongly induces polyubiquitination and internalization of the full-length p185-ERBB2, and promotes its...

Insulin receptor-related receptor in the pancreas and in a β-cell line

Igor E. Deyev, Oxana V. Serova, Andrey A. Mozhaev, Tatiana N. Erokhina, Marì Regoli, Alexander G. Petrenko & Eugenio Bertelli
Insulin receptor-related receptor (IRR) belongs to the family of the insulin receptor (IR) along with the IR itself and the insulin-like growth factor receptor. Whereas the ligands of the latter receptors are known, identification of an IRR ligand has eluded investigations so that IRR has been considered an orphan receptor. A recent breakthrough in the understanding of IRR functional role came from the finding that IRR can be activated by mildly alkali media in absence...

Engineered vesiscles from gingival stem cells: a new approach in 3D printed bone tissue regeneration

Francesca Diomede, Sabrina Giacoppo, Ilaria Merciaro, Paolo Cardelli, Placido Bramanti, Antonella Fontana, Emanuela Mazzon & Oriana Trubiani
In the bone regeneration field, properties of 3D scaffold could be improved using cellular and their released products. Even if previously documented, poly-(lactide) (PLA) scaffolds were not thoroughly evaluated for their design-related characteristics. The aim of the study was to investigate the properties of 3D printed PLA scaffolds for bone regeneration obtained through 3D printing, evaluating the differences in terms of structural properties, in vitro and in vivo cellular responses induced by different scaffold structures....

Morphological and immunoistochemical evaluation of cadaveric gingival specimens to estimate the post-mortem Interval

Mirella Falconi, Paolo Fais, Lucia Manzoli, Gabriella Teti, Maria Carla Mazzotti & Susi Pelotti
The estimation of the post-mortem interval (PMI) is a critical step in forensic medicine and remains one of the most challenging variables to determine. In general, the numerous methods currently used in estimating post-mortem interval yield to large post-mortem windows, are influenced by several factors and sometime contradict each other. With the aim to obtain a much more accurate determination of the post-mortem interval we combined morphological ultrastructural and immunoistochemical analyses to reach a more...

Innovative teaching methods. Effective solutions to complex contests

Chiara Panciroli, Laura Corazza, Patrizio Vignola, Elena Marcato & Daniela Leone
Innovative teaching methods can be thought of as a complex device, where cognitive, emotional and procedural elements are interconnected. Teaching is also defined as innovative when it is possible to find successful and effective solutions to complex contests in terms of mediation and sustainability. This abstract investigates three different teaching methods (Flipped Classroom, Cooperative Learning and Project Based Learning) and the complex system of Competency-based Learning they refer to. Furthermore, not only does it examine...

Working in the school as a complex organization. Theoretical perspectives, models, professionalism for the Secondary School

Lucia Balduzzi, Giovanna Del Gobbo & Loredana Perla
Lavorare nell’organizzazione complessa scuola. Prospettive teoriche, modelli, professionalità per la Secondaria di I e II grado

A morphometric and radiological study of the distal end of femur in West Bengal population

Ananya Biswas & Santanu Bhattacharya
The distal end of the femur has immense importance from the anatomical, functional and clinical point of view. The present study was carried out to assess the individual condylar dimension, maximum bicondylar width, size of intercondylar notch and bicondylar angle on dry femurs and on digital radiographic plates among the population of eastern India. The study was carried out bilaterally on seventy adult dry femurs of unknown sex and also on fifty digital radiographic plates...

Giovanni Battista Della Porta’s (1535?-1615) study on ocular anatomy

Konstantinos Laios, Marilita M. Moschos & George Androutsos
Giovanni Battista Della Porta (1535?-1615) was a polymath who dealt with many scientific fields. Among his studies very important are those concerning optics and the physiology of vision. Due to his interest in optics it was inevitable to deal also with ocular anatomy. His anatomical description of the eye gives us a lot of information about the development of the knowledge concerning ocular anatomy of his time.


Magdalena Andreea Strachinescu Olteanu & Eddy Hartog
TECHNE - Journal of Technology for Architecture and Environment, TECHNE: Special Series Vol. 1

È solo la lingua che fa eguali. Don Lorenzo Milani sacerdote e maestro

Bruna Bocchini Camaiani
Don Lorenzo Milani (1923-1967) was an exceptional figure in Italy’s Church of the 1950s and 1960s. Italy’s new democracy - he believed - needed citizens educated in a new way and the Church new devotees with a minimum of logical and linguistic capabilities. The loss of influence of the Church was not a consequence of Communist propaganda but of widespread ignorance and illiteracy in the population and of inadequate formation of the priests themselves. His...

Artigiani digitali. Per un modello innovativo di work related learning e di alternanza scuola-lavoro

Walter Rinaldi
The article compares the different international institutional models of work related learning and of work-school alternation and it deals with the innovative and community experiences related to the transformations of job, of professions, of knowledge, of culture.

Toward the smart city and beyond

Ernesto Antonini & Elena Mussinelli
TECHNE - Journal of Technology for Architecture and Environment, TECHNE: Special Series Vol. 1

Spheroids from human primary skin myofibroblasts as experimental system for myofibroblast deactivation studies

Angelica Avagliano, Giuseppina Granato, Maria R. Ruocco, Stefania Masone, Valeria Imparato, Stefania Montagnani & Alessandro Arcucci
Myofibroblasts are activated fibroblasts, involved in tissue repair and cancer, characterized by de novo expression of alpha smooth muscle actin (α-SMA), increased secretion of growth factors and immunoregulatory phenotype [1]. At the end of wound healing myofibroblasts undergo apoptotic cell death, whereas in vitro they are also subjected to a programmed necrosis-like cell death, termed nemosis, associated with cyclooxygenase-2 (COX-2) expression induction and inflammatory response [1,2]. Moreover, fibroblasts form clusters during wound healing, fibrotic states...

PI3Kα-selective inhibitor alpelisib (BYL719), may be effective as anticancer agents in Rhabdomyosarcoma

Alberto Bavelloni, Manuela Piazzi, Giulia Ramazzotti, Roberta Fiume, William Blalock & Irene Faenza
Rhabdomyosarcoma (RMS) is a highly malignant and metastatic pediatric cancer that arises from the skeletal muscle. Recent studies have identified an important role of AKT signaling in RMS progression. This suggests targeting components of the PI3K/Akt pathway may be an effective therapeutic strategy. Here, we investigated the in vitro activity of the class I PI3K inhibitors [1] in human rhabdomyosarcoma cell lines (embryonal rhabdomyosarcoma RD and A204, alveolar rhabdomyosarcoma SJCRH30). We used a panel of...

Prevalence of pedal symphalangism in Turkish population. A radiographic survey

Selahattin Ozyurek, Ozkan Kose, Mustafa Celiktas, Bogachan Tokatman, Aziz Atik & Omer Faruk Kilicaslan
Objective: The purpose of this study is to investigate the prevalence of biphalengeal toes in Turkish population and to analyze the differences between genders and symmetry patterns. Material and methods: Bilateral foot radiographs of 279 subjects with a mean age of 40.7±16.3 (range, 18-78) years were reviewed to detect presence of pedal biphalangism. There were 146 (52.3%) female and 133 (47.7%) male subjects. Results: The overall prevalence of biphalangeal 3rd toe was 0.9%, 4th toe...

Anatomical considerations on surgical implications of corona mortis: an Indian study

Minnie Pillay, Tintu T. Sukumaran & Mahendran Mayilswamy
The blood vessels traversing the superior pubic ramus are usually vascular connections between obturator and external iliac systems of vessels. Dislocated fractures or iatrogenic injury can cause life threatening bleeding and hence these vascular anomalies are referred to as corona mortis meaning ‘crown of death’. Except for a case report, no study on corona mortis has been attempted in India so far and hence the present study was intended at exploring the possible variations, both...

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