4,196 Works


Charles L. Mee
Nearly all the awful things you can imagine, and then, at the end, a prayer. [10 actors]

The Four Seasons

Charles L. Mee
Trees with buds on the branches, and, then, later on, there will be summer leaves, and then, fall foliage, and, at the end, bare branches, and then, finally, spring blossoms. And the clothes the actors wear: at first the clothes of spring, then—if they are not all naked, like a nineteenth century French painting of a picnic—they are in bikinis and summer shorts, and then the sweaters of autumn, and, finally, winter overcoats and gloves...

The Life of George Washington

Charles L. Mee
A backyard barbecue with George Washington, Walt Whitman, Jack Kerouac, Allen Ginsberg, Aunt Eller, Curly, Bambi, and some guys dancing with rifles and some bloggers—because: how it is, always, when you check into the old folks' home, or you go to your deathbed, you think you're finished, but you're not. And George Washington: he lives on in all of us.

Our Times: On the Street Where I Live

Charles L. Mee
In the olden days people lived in small towns and villages. These days they live in cities. And so, when they go out the front door to get a cup of coffee somewhere, this is how the world looks. [8 or 10 actors or more]

MHC Class I Expression by Donor Hematopoietic Stem Cells Is Required to Prevent NK Cell Attack in Allogeneic, but Not Syngeneic Recipient Mice

Yuichi Hirata, Hao Wei Li, Kazuko Takahashi, Hiroshi Ishii, Megan Sykes & Joji Fujisaki
NK cells resist engraftment of syngeneic and allogeneic bone marrow (BM) cells lacking major histocompatibility (MHC) class I molecules, suggesting a critical role for donor MHC class I molecules in preventing NK cell attack against donor hematopoietic stem and progenitor cells (HSPCs), and their derivatives. However, using high-resolution in vivo imaging, we demonstrated here that syngeneic MHC class I knockout (KO) donor HSPCs persist with the same survival frequencies as wild-type donor HSPCs. In contrast,...

Generalized Framework of OKID for Linear State-Space Model Identification

Francesco Vicario, Minh Q. Phan, Richard W. Longman & Raimondo Betti
This paper presents a generalization of observer/Kalman filter identification (OKID). OKID is a method for the simultaneous identification of a linear dynamical system and the associated Kalman filter from input-output measurements corrupted by noise. OKID was originally developed at NASA as the OKID/ERA algorithm. Recent work showed that ERA is not the only way to complete the OKID process and paved the way to the generalization of OKID as an approach to linear system identification....

Variable window binding for mutually exclusive alternative splicing

Dimitris Anastassiou, Hairuo Liu & Vinay Varadan
Background: Genes of advanced organisms undergo alternative splicing, which can be mutually exclusive, in the sense that only one exon is included in the mature mRNA out of a cluster of alternative choices, often arranged in a tandem array. In many cases, however, the details of the underlying biologic mechanisms are unknown. Results: We describe 'variable window binding' - a mechanism used for mutually exclusive alternative splicing by which a segment ('window') of a conserved...

Whole-genome association analysis to identify markers associated with recombination rates using single-nucleotide polymorphisms and microsatellites

Song Huang, Shuang Wang, Nianjun Liu, Liang Chen, Cheongeun Oh & Hongyu Zhao
Recombination during meiosis is one of the most important biological processes, and the level of recombination rates for a given individual is under genetic control. In this study, we conducted genome-wide association studies to identify chromosomal regions associated with recombination rates. We analyzed genotype data collected on the pedigrees in the Collaborative Study on the Genetics on Alcoholism data provided by Genetic Analysis Workshop 14. A total of 315 microsatellites and 10,081 single-nucleotide polymorphisms from...

Whole-genome linkage analysis in mapping alcoholism genes using single-nucleotide polymorphisms and microsatellites

Shuang Wang, Song Huang, Nianjun Liu, Liang Chen, Cheongeun Oh & Hongyu Zhao
There is currently a great interest in using single-nucleotide polymorphisms (SNPs) in genetic linkage and association studies because of the abundance of SNPs as well as the availability of high-throughput genotyping technologies. In this study, we compared the performance of whole-genome scans using SNPs with microsatellites on 143 pedigrees from the Collaborative Studies on Genetics of Alcoholism provided by Genetic Analysis Workhsop 14. A total of 315 microsatellites and 10,081 SNPs from Affymetrix on 22...

Evaluation of Integrated Community Case Management in Eight Districts of Central Uganda

Denis Mubiru, Robert Byabasheija, John Baptist Bwanika, Joslyn Edelstein Meier, Godfrey Magumba, Flavia Mpanga Kaggwa, Jackson Ojera Abusu, Alex Chono Opio, Charles Clarke Lodda, Jaanki Patel & Theresa Diaz
Objective Evidence is limited on whether Integrated Community Case Management (iCCM) improves treatment coverage of the top causes of childhood mortality (acute respiratory illnesses (ARI), diarrhoea and malaria). The coverage impact of iCCM in Central Uganda was evaluated. Methods Between July 2010 and December 2012 a pre-post quasi-experimental study in eight districts with iCCM was conducted; 3 districts without iCCM served as controls. A two-stage household cluster survey at baseline (n = 1036 and 1042)...

The Influence of Drivers and Barriers on Urban Adaptation and Mitigation Plans—An Empirical Analysis of European Cities

Diana Reckien, Johannes Flacke, Marta Olazabal & Oliver Heidrich
Cities are recognised as key players in global adaptation and mitigation efforts because the majority of people live in cities. However, in Europe, which is highly urbanized and one of the most advanced regions in terms of environmental policies, there is considerable diversity in the regional distribution, ambition and scope of climate change responses. This paper explores potential factors contributing to such diversity in 200 large and medium-sized cities across 11 European countries. We statistically...

A Multi-Area Stochastic Model for a Covert Visual Search Task

Michael A. Schwemmer, Samuel F. Feng, Philip J. Holmes, Jacqueline Gottlieb & Jonathan D. Cohen
Decisions typically comprise several elements. For example, attention must be directed towards specific objects, their identities recognized, and a choice made among alternatives. Pairs of competing accumulators and drift-diffusion processes provide good models of evidence integration in two-alternative perceptual choices, but more complex tasks requiring the coordination of attention and decision making involve multistage processing and multiple brain areas. Here we consider a task in which a target is located among distractors and its identity...

On the Significance of Psychodynamic Discourse for the Field of Consciousness Studies

Robin S. Brown
Despite the obvious confluence of concerns between psychodynamic psychology and the emerging field of consciousness studies, the extent to which psychodynamic thinking has factored into the consciousness literature has been limited. With widespread interest in “the unconscious” having significantly diminished, the present paper asks what might be implied in the shift towards the notion of “consciousness”—what about this cross-disciplinary designation has come to attract attention not only within the academic world, but also in the...

Blockade of Treg derived TGF-β abrogates suppression of effector T cell function within the tumor microenvironment

Sadna Budhu, David Schaer, Yongbiao Li, Alan Houghton, Samuel C. Silverstein, Taha Merghoub & Jedd Wolchok
Regulatory T cells (Treg) play a role in suppression of anti-melanoma immunity; however, the exact mechanism is poorly understood. Through intravital two photon microscopy, we found that Pmel-1 effectors engage in cell-cell interactions with tumor resident Tregs. To determine if contact between Tregs and T effectors (Teff) hinders killing of tumor cells in vivo, we utilized ex-vivo three-dimensional collagen-fibrin gel cultures of B16 melanoma cells. Collagen-fibrin gel cultures recapitulated the in vivo suppression, rendering the...

Toward integrative cancer immunotherapy: targeting the tumor microenvironment

Leisha A. Emens, Samuel C. Silverstein, Samir Khleif, Francesco M. Marincola & Jérôme Galon
The development of cancer has historically been attributed to genomic alterations of normal host cells. Accordingly, the aim of most traditional cancer therapies has been to destroy the transformed cells themselves. There is now widespread appreciation that the progressive growth and metastatic spread of cancer cells requires the cooperation of normal host cells (endothelial cells, fibroblasts, other mesenchymal cells, and immune cells), both local to, and at sites distant from, the site at which malignant...

CD11b/CD18 mediates production of reactive oxygen species by mouse and human macrophages adherent to matrixes containing oxidized LDL

Jens Husemann, Amrom Obstfeld, Maria Febbraio, Tatsuhiko Kodama & Samuel C. Silverstein
Production of reactive oxygen species (ROS) and other proinflammatory substances by macrophages adherent to matrix proteins that contain or have been modified by oxidized LDL (oxLDL) may play an important role in atherogenesis. In vitro, human macrophages adhere to matrixes containing oxLDL via scavenger receptors and are signaled to produce ROS partly by interactions of the class B scavenger receptor (SR-B) CD36 with ligands on the matrix. In this report, we show that macrophages from...

Are We Ready for Mass Fatality Incidents? Preparedness of the US Mass Fatality Infrastructure

Jacqueline A. Merrill, Mark Orr, Daniel Chen, Qi Zhi & Robyn R. Gershon
Objective To assess the preparedness of the US mass fatality infrastructure, we developed and tested metrics for 3 components of preparedness: organizational, operational, and resource sharing networks. Methods In 2014, data were collected from 5 response sectors: medical examiners and coroners, the death care industry, health departments, faith-based organizations, and offices of emergency management. Scores were calculated within and across sectors and a weighted score was developed for the infrastructure. Results A total of 879...

The Life and Death of Mass Media

Natan Dotan
There is a paradox in our understanding of the media today. Popular accounts often proclaim that mass media is dead while newspapers routinely report that new Hollywood box-office records have been smashed. In this dissertation I aim to resolve this paradox and to determine whether or not mass media is in fact in terminal decline. I propose two new concepts - the principle of cheap publicity and the mass media tendency - and I use...

What Happened to Goldman Sachs: An Insider's Story of Organizational Drift and its Unintended Consequences

Steven George Mandis
This is the story of the slow evolution of Goldman Sachs - addressing why and how the firm changed from an ethical standard to a legal one as it grew to be a leading global corporation. In What Happened to Goldman Sachs, Steven G. Mandis uncovers the forces behind what he calls Goldman's "organizational drift." Drawing from his firsthand experience; sociological research; analysis of SEC, congressional, and other filings; and a wide array of interviews...

Implications of Contemporary Bluegrass Music Performance at and around a New York City Jam Session

Jonathan Tobias King
Bluegrass as it is played in the United States today is not simply a resistant category of country music, but performs a particular and emergent view of past/present relations. More than a "micromusic" mediating between "supercultures" and "subcultures" (in Mark Slobin's terms 1993), in fact bluegrass's complex history resists simple top down or bottom up perspectives, articulating a distinct space of authenticity. Active `genre tending' in a jam setting poetically articulates emergent social relations, in...

Helicobacter pylori Infection Induces Anemia, Depletes Serum Iron Storage, and Alters Local Iron-Related and Adult Brain Gene Expression in Male INS-GAS Mice

Monika Burns, Sureshkumar Muthupalani, Vasudevan Bakthavatchalu, Zhongming Ge, Timothy C. Wang, Kathleen Ennis, Catriona Cunningham, Michael Georgieff & James G. Fox
Iron deficiency anemia (IDA) affects > 500 million people worldwide, and is linked to impaired cognitive development and function in children. Helicobacter pylori, a class 1 carcinogen, infects about half of the world’s population, thus creating a high likelihood of overlapping risk. This study determined the effect of H. pylori infection on iron homeostasis in INS-GAS mice. Two replicates of INS-GAS/FVB male mice (n = 9-12/group) were dosed with H. pylori (Hp) strain SS1 or...

Reduced El Niño–Southern Oscillation during the Last Glacial Maximum

Heather Louise Ford, A. Christina Ravelo & Pratigya Jeremy Polissar
El Niño–Southern Oscillation (ENSO) is a major source of global interannual variability, but its response to climate change is uncertain. Paleoclimate records from the Last Glacial Maximum (LGM) provide insight into ENSO behavior when global boundary conditions (ice sheet extent, atmospheric partial pressure of CO2) were different from those today. In this work, we reconstruct LGM temperature variability at equatorial Pacific sites using measurements of individual planktonic foraminifera shells. A deep equatorial thermocline altered the...

Molecular Evolution and Genetic Analysis of the Major Capsid Protein VP1 of Duck Hepatitis A Viruses: Implications for Antigenic Stability

Xiuli Ma, Zizhang Sheng, Bing Huang, Lihong Qi, Yufeng Li, Kexiang Yu, Cunxia Liu, Zhuoming Qin, Dan Wang, Minxun Song & Feng Li
The duck hepatitis A virus (DHAV), a member of the family Picornaviridae, is the major cause of outbreaks with high mortality rates in young ducklings. It has three distinctive serotypes and among them, serotypes 1 (DHAV-1) and 3 (DHAV-3) were recognized in China. To investigate evolutionary and antigenic properties of the major capsid protein VP1 of these two serotypes, a primary target of neutralizing antibodies, we determined the VP1 coding sequences of 19 DHAV-1 (spanning...

Protection from Lethal Gram-Positive Infection by Macrophage Scavenger Receptor–Dependent Phagocytosis

Christian A. Thomas, Yongmei Li, Tatsuhiko Kodama, Hiroshi Suzuki, Samuel C. Silverstein & Joseph El Khoury
Infections with gram-positive bacteria are a major cause of morbidity and mortality in humans. Opsonin-dependent phagocytosis plays a major role in protection against and recovery from gram-positive infections. Inborn and acquired defects in opsonin generation and/or recognition by phagocytes are associated with an increased susceptibility to bacterial infections. In contrast, the physiological significance of opsonin-independent phagocytosis is unknown. Type I and II class A scavenger receptors (SR-AI/II) recognize a variety of polyanions including bacterial cell...

Complementary roles for scavenger receptor A and CD36 of human monocyte-derived macrophages in adhesion to surfaces coated with oxidized low-density lipoproteins and in secretion of H2O2

Horst Maxeiner, Jens Husemann, Christian A. Thomas, Joseph El Khoury, John D. Loike & Samuel C. Silverstein
Oxidized low-density lipoprotein (oxLDL) is considered one of the principal effectors of atherogenesis. To explore mechanisms by which oxLDL affects human mononuclear phagocytes, we incubated these cells in medium containing oxLDL, acetylated LDL (acLDL), or native LDL, or on surfaces coated with these native and modified lipoproteins. The presence of soluble oxLDL, acLDL, or native LDL in the medium did not stimulate H2O2 secretion by macrophages. In contrast, macrophages adherent to surfaces coated with oxLDL...

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  • Duke University