286,291 Works

Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells

Ernst Böhm, Birgit Seyfried, Michael Dockal, Michael Graninger, Meinhard Hasslacher, Marianne Neurath, Christian Konetschny, Peter Matthiessen, Artur Mitterer & Friedrich Scheiflinger
Abstract Background & Methods Recombinant factor VII (rFVII), the precursor molecule for recombinant activated FVII (rFVIIa), is, due to its need for complex post translational modifications, produced in mammalian cells. To evaluate the suitability of a human cell line in order to produce rFVII with post-translational modifications as close as possible to pdFVII, we compared the biochemical properties of rFVII synthesized in human embryonic kidney-derived (HEK)293 cells (HEK293rFVII) with those of rFVII expressed in Chinese...

Tracing the origin of disseminated tumor cells in breast cancer using single-cell sequencing

Jonas Demeulemeester, Parveen Kumar, Elen Møller, Silje Nord, David Wedge, April Peterson, Randi Mathiesen, , Masoud Zamani Esteki, Koen Theunis, Elia Fernandez Gallardo, , Elin Borgen, Lars Baumbusch, , Kevin White, Vessela Kristensen, Peter Van Loo, Thierry Voet & Bjørn Naume
Abstract Background Single-cell micro-metastases of solid tumors often occur in the bone marrow. These disseminated tumor cells (DTCs) may resist therapy and lay dormant or progress to cause overt bone and visceral metastases. The molecular nature of DTCs remains elusive, as well as when and from where in the tumor they originate. Here, we apply single-cell sequencing to identify and trace the origin of DTCs in breast cancer. Results We sequence the genomes of 63...

Extending the PRISMA statement to equity-focused systematic reviews (PRISMA-E 2012): explanation and elaboration

Vivian Welch, Mark Petticrew, Jennifer Petkovic, David Moher, Elizabeth Waters, Howard White & Peter Tugwell
Abstract Background The promotion of health equity, the absence of avoidable and unfair differences in health outcomes, is a global imperative. Systematic reviews are an important source of evidence for health decision-makers, but have been found to lack assessments of the intervention effects on health equity. The Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) is a 27 item checklist intended to improve transparency and reporting of systematic reviews. We developed an equity extension...

Diagnosing sepsis is subjective and highly variable: a survey of intensivists using case vignettes

, Sameer Kadri, Robert Danner, Anthony Suffredini, Anthony Massaro, Barrett Kitch, Grace Lee & Michael Klompas
Abstract Background Sepsis is the focus of national quality improvement programs and a recent public reporting measure from the Centers for Medicare and Medicaid Services. However, diagnosing sepsis requires interpreting nonspecific signs and can therefore be subjective. We sought to quantify interobserver variability in diagnosing sepsis. Methods We distributed five case vignettes of patients with suspected or confirmed infection and organ dysfunction to a sample of practicing intensivists. Respondents classified cases as systemic inflammatory response...

Post-paralysis tyrosine kinase inhibition with masitinib abrogates neuroinflammation and slows disease progression in inherited amyotrophic lateral sclerosis

Emiliano Trias, Sofía Ibarburu, Romina Barreto-Núñez, Joël Babdor, , Matthias Guillo, Laurent Gros, Patrice Dubreuil, Pablo Díaz-Amarilla, Patricia Cassina, Laura Martínez-Palma, Ivan Moura, Joseph Beckman, Olivier Hermine & Luis Barbeito
Abstract Background In the SOD1G93A mutant rat model of amyotrophic lateral sclerosis (ALS), neuronal death and rapid paralysis progression are associated with the emergence of activated aberrant glial cells that proliferate in the degenerating spinal cord. Whether pharmacological downregulation of such aberrant glial cells will decrease motor neuron death and prolong survival is unknown. We hypothesized that proliferation of aberrant glial cells is dependent on kinase receptor activation, and therefore, the tyrosine kinase inhibitor masitinib...

CoDiab-VD: protocol of a prospective population-based cohort study on diabetes care in Switzerland

Emilie Zuercher, Julie Bordet, Bernard Burnand & Isabelle Peytremann-Bridevaux
Abstract Background Diabetes represents an increasing health burden worldwide. In 2010, the Public Health Department of the canton of Vaud (Switzerland) launched a regional diabetes programme entitled “Programme cantonal Diabète” (PcD), with the objectives to both decrease the incidence of diabetes and improve care for patients with diabetes. The cohort entitled CoDiab-VD emerged from that programme. It specifically aimed at following quality of diabetes care over time, at evaluating the coverage of the PcD within...

Merino and Merino-derived sheep breeds: a genome-wide intercontinental study

Elena Ciani, Emiliano Lasagna, , Ingrid Alloggio, Fabio Marroni, Simone Ceccobelli, Juan Delgado Bermejo, Francesca Sarti, James Kijas, Johannes Lenstra & Fabio Pilla
Abstract Background Merino and Merino-derived sheep breeds have been widely distributed across the world, both as purebred and admixed populations. They represent an economically and historically important genetic resource which over time has been used as the basis for the development of new breeds. In order to examine the genetic influence of Merino in the context of a global collection of domestic sheep breeds, we analyzed genotype data that were obtained with the OvineSNP50 BeadChip...

In patients with metastatic breast cancer the identification of circulating tumor cells in epithelial-to-mesenchymal transition is associated with a poor prognosis

Michela Bulfoni, Lorenzo Gerratana, Fabio Del Ben, Stefania Marzinotto, Marisa Sorrentino, Matteo Turetta, Giacinto Scoles, Barbara Toffoletto, Miriam Isola, Carlo Beltrami, Carla Di Loreto, Antonio Beltrami, Fabio Puglisi & Daniela Cesselli
Abstract Background Although recent models suggest that the detection of Circulating Tumor Cells (CTC) in epithelial-to-mesenchymal transition (EM CTC) might be related to disease progression in metastatic breast cancer (MBC) patients, current detection methods are not efficient in identifying this subpopulation of cells. Furthermore, the possible association of EM CTC with both clinicopathological features and prognosis of MBC patients has still to be demonstrated. Aims of this study were: first, to optimize a DEPArray-based protocol...

Intravenous administration of silver nanoparticles causes organ toxicity through intracellular ROS-related loss of inter-endothelial junction

Hua Guo, Jing Zhang, Mary Boudreau, Jie Meng, Jun-Jie Yin, Jian Liu &
Abstract Background Administration of silver nanoparticles (AgNPs) to mice could result in their distribution and accumulation in multiple organs, with notable prominence in liver, lungs, and kidneys. However, how AgNPs transport through blood vesicular system to reach the target organs is unclear, and the precise differences in the mechanisms of toxicity between AgNPs and silver ions still remain elusive. In the present research, the pathological changes on these target organs with a focus on inter-endothelial...

Online Labour Index: Measuring the Online Gig Economy for Policy and Research

Otto Kässi, Martin Hadley & Vili Lehdonvirta
Data repository for the data underlying the Online Labour Index. See http://ilabour.oii.ox.ac.uk online-labour-index/ for details.

Supplementary material from \"Semisynthesis and insecticidal activity of some novel fraxinellone-based thioethers containing 1,3,4-oxadiazole moiety\"

Yong Guo, , , Qian Zhang, Yi Wang, Yi Zhao, , Ming Ding &
Two series of novel fraxinellone-based thioethers containing 1,3,4-oxadiazole moiety were prepared as insecticidal agents against the oriental armyworm, Mythimna separata Walker. The structural assignment was based on the spectroscopic and X-ray analysis data. Among all the target compounds, compounds 4b, 4 k, 5b, 5j and 5 k exhibited more potent insecticidal activity with final mortality rates (FMRs) of more than 65%, especially 4 k with the FMR of 75.9%, when compared with toosendanin. Some interesting results of structure–activity...

Supplementary material from \"New composite thixotropic hydrogel composed of a polymer hydrogelator and a nanosheet\"

Yutaka Ohsedo, Masashi Oono, , Hisayuki Watanabe & Nobuyoshi Miyamoto
A composite gel composed of a water-soluble aromatic polyamide hydrogelator and the nanosheet Laponite®, a synthetic layered silicate, was produced and found to exhibit thixotropic behaviour. Whereas the composite gel contains the gelator at the same concentration equal to the molecular gel made by the gelator only, the composite gel becomes softer thixotropic gel comparing to molecular gel made by the gelator only. This may be due to the reason that a bundle of polymer...

Factors influencing decision of general practitioners and managers to train and employ a nurse practitioner or physician assistant in primary care: a qualitative study

Mieke Van Der Biezen, Emmy Derckx, Michel Wensing & Miranda Laurant
Abstract Background Due to the increasing demand on primary care, it is not only debated whether there are enough general practitioners (GPs) to comply with these demands but also whether specific tasks can be performed by other care providers. Although changing the workforce skill mix care by employing Physician Assistants (PAs) and Nurse Practitioners (NPs) has proven to be both effective and safe, the implementation of those professionals differs widely between and within countries. To...

Selection on the mitochondrial ATP synthase 6 and the NADH dehydrogenase 2 genes in hares (Lepus capensis L., 1758) from a steep ecological gradient in North Africa

Hichem Ben Slimen, Helmut Schaschl, Felix Knauer & Franz Suchentrunk
Abstract Background Recent studies of selection on mitochondrial (mt) OXPHOS genes suggest adaptation due mainly to environmental variation. In this context, Tunisian hares that display several external phenotypes with phylogenetically rather homogenous gene pool and shallow population structure provide a good precondition to detect positive selection on mt genes related to environmental/climatic variation, specifically ambient temperature and precipitation. Results We used codon-based methods along with population genetic data to test for positive selection on ATPase...

A randomized exploratory phase 2 study in patients with chemotherapy-related peripheral neuropathy evaluating whole-body vibration training as adjunct to an integrated program including massage, passive mobilization and physical exercises

Stefan Schönsteiner, Heidi Bauder Mißbach, Axel Benner, Silja Mack, Thomas Hamel, Michael Orth, Bernhard Landwehrmeyer, Sigurd Süßmuth, Carolin Geitner, Regine Mayer-Steinacker, Anneliese Riester, Andrea Prokein, Elfriede Erhardt, Jelena Kunecki, Anna Eisenschink, Rainer Rawer, Hartmut Döhner, Elisabeth Kirchner & Richard Schlenk
Abstract Background Chemotherapy-induced polyneuropathy (CIPN) is a common toxicity after chemotherapy, immunomodulatory drugs or proteasome inhibitors, which is difficult to treat and may also have impact on quality of life. The objective of the study was to evaluate whole-body vibration (WBV) on the background of an integrated program (IP) including massage, passive mobilization and physical exercises on CIPN. Patients and methods In an exploratory phase-2 study patients with CIPN (NCI CTC grade 2/3) were randomized...

Primary care patient experience and cancer screening uptake among women: an exploratory cross-sectional study in a Japanese population

Takuya Aoki & Machiko Inoue
Abstract Background Patient experience and clinical quality, which are represented by preventive care measures such as cancer screening, are both widely used for the evaluation of primary care quality. The aim of this study was to examine the association between patient experience and cancer screening uptake among women in a Japanese population. Methods We conducted a cross-sectional mail survey. The questionnaire was sent to 1000 adult female residents randomly selected from a basic resident register...

Building a model for disease classification integration in oncology, an approach based on the national cancer institute thesaurus

Vianney Jouhet, Fleur Mougin, Bérénice Bréchat & Frantz Thiessard
Abstract Background Identifying incident cancer cases within a population remains essential for scientific research in oncology. Data produced within electronic health records can be useful for this purpose. Due to the multiplicity of providers, heterogeneous terminologies such as ICD-10 and ICD-O-3 are used for oncology diagnosis recording purpose. To enable disease identification based on these diagnoses, there is a need for integrating disease classifications in oncology. Our aim was to build a model integrating concepts...

The importance of selection in the evolution of blindness in cavefish

Reed A. Cartwright, Rachel S. Schwartz, Alexandra L. Merry & Megan M. Howell
Abstract Background Blindness has evolved repeatedly in cave-dwelling organisms, and many hypotheses have been proposed to explain this observation, including both accumulation of neutral loss-of-function mutations and adaptation to darkness. Investigating the loss of sight in cave dwellers presents an opportunity to understand the operation of fundamental evolutionary processes, including drift, selection, mutation, and migration. Results Here we model the evolution of blindness in caves. This model captures the interaction of three forces: (1) selection...

Characteristics of human adipose derived stem cells in scleroderma in comparison to sex and age matched normal controls: implications for regenerative medicine

Michelle Griffin, Caroline Ryan, Omar Pathan, David Abraham, Christopher Denton & Peter Butler
Abstract Background Adipose-derived stem cells (ADSCs) are emerging as an alternative stem cell source for cell-based therapies. Recent data suggest that autologous ADSC-enriched micrografting improves the effects of facial involvement in systemic sclerosis (SSc). We have extensively characterised ADSCs from SSc patients and compared their phenotype and function to healthy age- and sex-matched control ADSCs. Methods ADSCs were isolated and characterised from a cohort of six SSc patients (ADSC-SSc) and were compared to six healthy...

Differential long noncoding RNA/mRNA expression profiling and functional network analysis during osteogenic differentiation of human bone marrow mesenchymal stem cells

, Rui Dong, Shu Diao, Juan Du, & Fu Wang
Abstract Background Mesenchymal stem cells (MSCs) are the most promising cell types for bone regeneration and repair due to their osteogenic potential. MSC differentiation is precisely regulated and orchestrated by the mechanical and molecular signals from the extracellular environment, involving complex pathways regulated at both the transcriptional and post-transcriptional levels. However, the potential role of long noncoding RNA (lncRNA) in the osteogenic differentiation of human MSCs remains largely unclear. Methods Here, we undertook the survey...

Anthocyanins encapsulated by PLGA@PEG nanoparticles potentially improved its free radical scavenging capabilities via p38/JNK pathway against Aβ1–42-induced oxidative stress

Faiz Amin, Shahid Shah, Haroon Badshah, & Myeong Kim
Abstract Background In order to increase the bioavailability of hydrophilic unstable drugs like anthocyanins, we employed a polymer-based nanoparticles approach due to its unique properties such as high stability, improved bioavailability and high water-soluble drug loading efficiency. Anthocyanins constitute a subfamily of flavonoids that possess anti-oxidative, anti-inflammatory and neuroprotective properties. However, anthocyanins are unstable because their phenolic hydroxyl groups are easily oxidized into quinones, causing a reduced biological activity. To overcome this drawback and improve...

Impact of weight maintenance and loss on diabetes risk and burden: a population-based study in 33,184 participants

Adina Feldman, Simon Griffin, Amy Ahern, , Lars Weinehall, Eva Fhärm, Margareta Norberg & Patrik Wennberg
Abstract Background Weight loss in individuals at high risk of diabetes is an effective prevention method and a major component of the currently prevailing diabetes prevention strategies. The aim of the present study was to investigate the public health potential for diabetes prevention of weight maintenance or moderate weight loss on a population level in an observational cohort with repeated measurements of weight and diabetes status. Methods Height, weight and diabetes status were objectively measured...

Analysis of small RNAs revealed differential expressions during pollen and embryo sac development in autotetraploid rice

Xiang Li, Muhammad Shahid, Juan Xia, , Na Fang, Lan Wang, , &
Abstract Background Partial pollen and embryo sac sterilities are the two main reasons for low fertility in autotetraploid rice. Our previous study revealed that small RNAs changes may associate with pollen fertility in autotetraploid rice. However, knowledge on comparative analysis between the development of pollen and embryo sac by small RNAs in autotetraploid rice is still unknown. In the present study, WE-CLSM (whole-mount eosin B-staining confocal laser scanning microscopy) and high-throughput sequencing technology was employed...

Whole-genome sequencing of a Plasmodium vivax clinical isolate exhibits geographical characteristics and high genetic variation in China-Myanmar border area

, Yue Wang, , Bin Xu, &
Abstract Background Currently in China, the trend of Plasmodium vivax cases imported from Southeast Asia was increased especially in the China-Myanmar border area. Driven by the increase in P. vivax cases and stronger need for vaccine and drug development, several P. vivax isolates genome sequencing projects are underway. However, little is known about the genetic variability in this area until now. Results The sequencing of the first P. vivax isolate from China-Myanmar border area (CMB-1)...

Mechanism of action of trabectedin in desmoplastic small round cell tumor cells

, , , , , , , , , , , , &
Abstract Background Desmoplastic small round cell tumor (DSRCT) is a rare and highly aggressive disease, that can be described as a member of the family of small round blue cell tumors. The molecular diagnostic marker is the t(11;22)(p13;q12) translocation, which creates an aberrant transcription factor, EWS-WT1, that underlies the oncogenesis of DSRCT. Current treatments are not very effective so new active drugs are needed. Trabectedin, now used as a single agent for the treatment of...

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