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123Movies

Indresh Singh
123Movies - First launched around 2015, Movies 123 had over 100 million users, unfortunately the site was shut down by the Motion Picture Association of America (MPAA) in March 2018.Let’s explore what 123Movies is and what makes it such a great and popular streaming site ahead of it’s competitors.123movie is an online streaming portal which is extremely popular, here's the complete 100% updated proxy list of 123Movies.

123Movies

Indresh Singh
123Movies - First launched around 2015, Movies 123 had over 100 million users, unfortunately the site was shut down by the Motion Picture Association of America (MPAA) in March 2018.Let’s explore what 123Movies is and what makes it such a great and popular streaming site ahead of it’s competitors.123movie is an online streaming portal which is extremely popular, here's the complete 100% updated proxy list of 123Movies.

Genome-scale metabolic modeling of human CD4+ T cells reveals ceramides as metabolic signature of Th17 and regulatory T-cell differentiation

Partho Sen, Alex M. Dickens, Ubaid Ullah, Syed Bilal Ahmad Andrabi, Mohd Moin Khan, Tanja Buchacher, Tuomas Lindeman, Victoria Hinkkanen, Esko Kemppainen, Omid Rasool, Tuulia Hyötyläinen, Riitta Lahesmaa & Matej Oresic
Poster at European RFMF Metabomeeting 2020 (Toulouse, January 22-24, 2020), no. 131https://rfmf-mpf-2020.sciencesconf.org/

ABSTRACTIntroduction. T helper (Th) cells play a pivotal role in cell-mediated immunity. During the development, T cells undergo metabolic remodeling that is essential for orchestrating the action of other immune cells. Technological and methodological innovation. In order to understand global metabolism during T cell development, we developed genome-scale metabolic models (GEMs) for human Th1, Th2 and Th17 subsets and T-regulatory cells (iTreg). Results. and...

Genome-scale metabolic modeling of human CD4+ T cells reveals ceramides as metabolic signature of Th17 and regulatory T-cell differentiation

Partho Sen, Alex M. Dickens, Ubaid Ullah, Syed Bilal Ahmad Andrabi, Mohd Moin Khan, Tanja Buchacher, Tuomas Lindeman, Victoria Hinkkanen, Esko Kemppainen, Omid Rasool, Tuulia Hyötyläinen, Riitta Lahesmaa & Matej Oresic
Poster at European RFMF Metabomeeting 2020 (Toulouse, January 22-24, 2020), no. 131https://rfmf-mpf-2020.sciencesconf.org/

ABSTRACTIntroduction. T helper (Th) cells play a pivotal role in cell-mediated immunity. During the development, T cells undergo metabolic remodeling that is essential for orchestrating the action of other immune cells. Technological and methodological innovation. In order to understand global metabolism during T cell development, we developed genome-scale metabolic models (GEMs) for human Th1, Th2 and Th17 subsets and T-regulatory cells (iTreg). Results. and...

Supplementary Figure.pdf

Jin Wang
Supplementa lFig. S1. GO and KEGG pathway analyses of the differentially expressed mRNAs. (A) Major enriched and significant GO-assessed up-regulated mRNAs.(B) Major enriched and significant GO-assessed down-regulated mRNAs. (C) The top ten significantly enriched pathways for the up-regulated mRNAs. (D) The top ten significantly enriched pathways for the down-regulated mRNAs. (E) The top 8 GO terms of biological process were significantly enriched for the 20 up-regulated genes in preeclampsia. (F) The top 9 GO terms...

Supplementary Figure.pdf

Jin Wang
Supplementa lFig. S1. GO and KEGG pathway analyses of the differentially expressed mRNAs. (A) Major enriched and significant GO-assessed up-regulated mRNAs.(B) Major enriched and significant GO-assessed down-regulated mRNAs. (C) The top ten significantly enriched pathways for the up-regulated mRNAs. (D) The top ten significantly enriched pathways for the down-regulated mRNAs. (E) The top 8 GO terms of biological process were significantly enriched for the 20 up-regulated genes in preeclampsia. (F) The top 9 GO terms...

Figure 2 -CyrtarachneSurfaceSpreading Comparison.ai from The moth specialist spider Cyrtarachne akirai uses prey scales to increase adhesion

Candido Diaz, Daniel Maksuta, Gaurav Amarpuri, Akio Tanikawa, Tadashi Miyashita, Ali Dhinojawala & Todd Blackledge
Contaminants decrease adhesive strength by interfering with substrate contact. Spider webs adhering to moths present an ideal model to investigate how natural adhesives overcome contamination because moths' sacrificial layer of scales rubs off on sticky silk, facilitating escape. However, Cyrtarachninae spiders have evolved gluey capture threads that adhere well to moths. Cyrtarachne capture threads contain large glue droplets oversaturated with water, readily flowing but also prone to drying out. Here, we compare the spreading and...

Figure 3 - Interspecies Spreading-LC on Moth Wings.ai from The moth specialist spider Cyrtarachne akirai uses prey scales to increase adhesion

Candido Diaz, Daniel Maksuta, Gaurav Amarpuri, Akio Tanikawa, Tadashi Miyashita, Ali Dhinojawala & Todd Blackledge
Contaminants decrease adhesive strength by interfering with substrate contact. Spider webs adhering to moths present an ideal model to investigate how natural adhesives overcome contamination because moths' sacrificial layer of scales rubs off on sticky silk, facilitating escape. However, Cyrtarachninae spiders have evolved gluey capture threads that adhere well to moths. Cyrtarachne capture threads contain large glue droplets oversaturated with water, readily flowing but also prone to drying out. Here, we compare the spreading and...

Figure 2 -CyrtarachneSurfaceSpreading Comparison.ai from The moth specialist spider Cyrtarachne akirai uses prey scales to increase adhesion

Candido Diaz, Daniel Maksuta, Gaurav Amarpuri, Akio Tanikawa, Tadashi Miyashita, Ali Dhinojawala & Todd Blackledge
Contaminants decrease adhesive strength by interfering with substrate contact. Spider webs adhering to moths present an ideal model to investigate how natural adhesives overcome contamination because moths' sacrificial layer of scales rubs off on sticky silk, facilitating escape. However, Cyrtarachninae spiders have evolved gluey capture threads that adhere well to moths. Cyrtarachne capture threads contain large glue droplets oversaturated with water, readily flowing but also prone to drying out. Here, we compare the spreading and...

Figure 3 - Interspecies Spreading-LC on Moth Wings.ai from The moth specialist spider Cyrtarachne akirai uses prey scales to increase adhesion

Candido Diaz, Daniel Maksuta, Gaurav Amarpuri, Akio Tanikawa, Tadashi Miyashita, Ali Dhinojawala & Todd Blackledge
Contaminants decrease adhesive strength by interfering with substrate contact. Spider webs adhering to moths present an ideal model to investigate how natural adhesives overcome contamination because moths' sacrificial layer of scales rubs off on sticky silk, facilitating escape. However, Cyrtarachninae spiders have evolved gluey capture threads that adhere well to moths. Cyrtarachne capture threads contain large glue droplets oversaturated with water, readily flowing but also prone to drying out. Here, we compare the spreading and...

MOESM1 of Method comparison for the direct enumeration of bacterial species using a chemostat model of the human colon

Ines Moura, Charmaine Normington, Duncan Ewin, Emma Clark, Mark Wilcox, Anthony Buckley & Caroline Chilton
Additional file 1: Figure S1. Mean gut microbiota populations of C. difficile based on the housekeeping gene gluD in vessel 3 of model A, B and C at the different stages of the experiment. Bar graphs represent the levels in log10 copies/μL measured by qPCR. CD, C. difficile; rCDI, recurrent CDI; FMT, faecal microbiota transplantation. Asterisks represent significant variations by qPCR between time points: *correspond to p

MOESM1 of Method comparison for the direct enumeration of bacterial species using a chemostat model of the human colon

Ines Moura, Charmaine Normington, Duncan Ewin, Emma Clark, Mark Wilcox, Anthony Buckley & Caroline Chilton
Additional file 1: Figure S1. Mean gut microbiota populations of C. difficile based on the housekeeping gene gluD in vessel 3 of model A, B and C at the different stages of the experiment. Bar graphs represent the levels in log10 copies/μL measured by qPCR. CD, C. difficile; rCDI, recurrent CDI; FMT, faecal microbiota transplantation. Asterisks represent significant variations by qPCR between time points: *correspond to p

MOESM6 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira & Mari Sogayar
Additional file 6: Figure S6. LC-MS/MS analysis. The rhRSPO1 sample obtained upon the optimized HPLC purification system was subjected to mass spectrometric analysis (LC-MS/MS) for identification of the glycosylation profile.

MOESM5 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira & Mari Sogayar
Additional file 5: Figure S5. rhRSPO1 glycosylation qualitative profile - Lectin Panel Assay. ELISA plate (NUNC-Immuno Plate/ MaxiSorp-NUNC) was coated with 200 ng/μL of purified rhRSPO1 and standard glycoproteins (carboxypeptidase, transferrin, fetuin, asylophetuin), as controls. Specific types of glycan residues in the rhRSPO1 molecule were detected by different lectins, namely: GNA (Terminal Mannose, (1–3), (1–6) or (1–2) linked to Mannose (N- or O-linked glycosylation)); SNA (Sialic Acid linked (2–6) to Galactose (N- or O-linked glycosylation));...

MOESM4 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira & Mari Sogayar
Additional file 4: Figure S4. rhRSPO1 in vitro bioactivity. C2C12 cells were treated with two doses of 200 ng/mL of rhRSPO1 obtained from different rhRSPO1-producing cell clones, for up to 5 days. The activity was measured by an alkaline phosphatase (ALP) colorimetric assay. Conditioned medium from HEK293 cells transfected with the empty pNU1 vector (C-) or 293 T cells expressing rhBMP7 were used as negative and positive controls, respectively. ANOVA statistical test (Tukey’s post hoc...

MOESM5 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira & Mari Sogayar
Additional file 5: Figure S5. rhRSPO1 glycosylation qualitative profile - Lectin Panel Assay. ELISA plate (NUNC-Immuno Plate/ MaxiSorp-NUNC) was coated with 200 ng/μL of purified rhRSPO1 and standard glycoproteins (carboxypeptidase, transferrin, fetuin, asylophetuin), as controls. Specific types of glycan residues in the rhRSPO1 molecule were detected by different lectins, namely: GNA (Terminal Mannose, (1–3), (1–6) or (1–2) linked to Mannose (N- or O-linked glycosylation)); SNA (Sialic Acid linked (2–6) to Galactose (N- or O-linked glycosylation));...

MOESM4 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira & Mari Sogayar
Additional file 4: Figure S4. rhRSPO1 in vitro bioactivity. C2C12 cells were treated with two doses of 200 ng/mL of rhRSPO1 obtained from different rhRSPO1-producing cell clones, for up to 5 days. The activity was measured by an alkaline phosphatase (ALP) colorimetric assay. Conditioned medium from HEK293 cells transfected with the empty pNU1 vector (C-) or 293 T cells expressing rhBMP7 were used as negative and positive controls, respectively. ANOVA statistical test (Tukey’s post hoc...

MOESM6 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira & Mari Sogayar
Additional file 6: Figure S6. LC-MS/MS analysis. The rhRSPO1 sample obtained upon the optimized HPLC purification system was subjected to mass spectrometric analysis (LC-MS/MS) for identification of the glycosylation profile.

MOESM2 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira & Mari Sogayar
Additional file 2: Figure S2. First step of rhRSPO1 purification by heparin affinity chromatography. Conditioned medium from an rhRSPO1-overproducing cell clone (Cl. L1) was used for protein purification. A- Chromatogram representing the purification process of rhRSPO1 with a heparin-affinity column in a three step segmented NaCl gradient. The blue line represents the absorbance at the 280 nm UV wavelength and the brown dotted line represents the conductance of the sample. B- Western blot of the...

MOESM3 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira & Mari Sogayar
Additional file 3: Figure S3. Second step of rhRSPO1 purification by molecular exclusion chromatography. The sample containing rhRSPO1 from step 2 of the heparin affinity column was used in this purification process. The blue line represents the absorbance at the 280 nm UV wavelength and the brown dotted line represents the conductance of the sample. A- Chromatogram representing the purification process of rhRSPO1 using a molecular exclusion column. B- Western Blot of the purification fractions,...

MOESM2 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira & Mari Sogayar
Additional file 2: Figure S2. First step of rhRSPO1 purification by heparin affinity chromatography. Conditioned medium from an rhRSPO1-overproducing cell clone (Cl. L1) was used for protein purification. A- Chromatogram representing the purification process of rhRSPO1 with a heparin-affinity column in a three step segmented NaCl gradient. The blue line represents the absorbance at the 280 nm UV wavelength and the brown dotted line represents the conductance of the sample. B- Western blot of the...

MOESM3 of Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells

Gabriel Levin, Bruna Koga, Gustavo Belchior, Ana Carreira & Mari Sogayar
Additional file 3: Figure S3. Second step of rhRSPO1 purification by molecular exclusion chromatography. The sample containing rhRSPO1 from step 2 of the heparin affinity column was used in this purification process. The blue line represents the absorbance at the 280 nm UV wavelength and the brown dotted line represents the conductance of the sample. A- Chromatogram representing the purification process of rhRSPO1 using a molecular exclusion column. B- Western Blot of the purification fractions,...

MOESM1 of IL-1-conferred gene expression pattern in ERα+ BCa and AR+ PCa cells is intrinsic to ERα− BCa and AR− PCa cells and promotes cell survival

Afshan Nawas, Mohammed Kanchwala, Shayna Thomas-Jardin, Haley Dahl, Kelly Daescu, Monica Bautista, Vanessa Anunobi, Ally Wong, Rachel Meade, Ragini Mistry, Nisha Ghatwai, Felix Bayerl, Chao Xing & Nikki Delk
Additional file 1: Figure S1. p62 and/or SOX9 are not required for cell survival in HR+ PCa and BCa cell lines. (A) LNCaP and (B) MCF7 cell lines were treated with 70 nM control siRNA (“c”), p62 siRNA, or SOX9 siRNA and after 1 day in siRNA, cells were treated with vehicle control (“veh”) or 25 ng/ml IL-1 for an additional 3 days. Western blot was performed to confirm p62 or SOX9 knockdown and MTT...

MOESM1 of IL-1-conferred gene expression pattern in ERα+ BCa and AR+ PCa cells is intrinsic to ERα− BCa and AR− PCa cells and promotes cell survival

Afshan Nawas, Mohammed Kanchwala, Shayna Thomas-Jardin, Haley Dahl, Kelly Daescu, Monica Bautista, Vanessa Anunobi, Ally Wong, Rachel Meade, Ragini Mistry, Nisha Ghatwai, Felix Bayerl, Chao Xing & Nikki Delk
Additional file 1: Figure S1. p62 and/or SOX9 are not required for cell survival in HR+ PCa and BCa cell lines. (A) LNCaP and (B) MCF7 cell lines were treated with 70 nM control siRNA (“c”), p62 siRNA, or SOX9 siRNA and after 1 day in siRNA, cells were treated with vehicle control (“veh”) or 25 ng/ml IL-1 for an additional 3 days. Western blot was performed to confirm p62 or SOX9 knockdown and MTT...

MOESM2 of IL-1-conferred gene expression pattern in ERα+ BCa and AR+ PCa cells is intrinsic to ERα− BCa and AR− PCa cells and promotes cell survival

Afshan Nawas, Mohammed Kanchwala, Shayna Thomas-Jardin, Haley Dahl, Kelly Daescu, Monica Bautista, Vanessa Anunobi, Ally Wong, Rachel Meade, Ragini Mistry, Nisha Ghatwai, Felix Bayerl, Chao Xing & Nikki Delk
Additional file 2: Figure S2. Verteporfin is cytotoxic for HR− BCa and PCa cell lines. (A) MDA-MB-231, BT549, PC3, and DU145 cell lines were treated with vehicle control, 2.5, 5, or 10 μM verteporfin for 1 day. Western blot analysis shows oligomerized p62 (p62*), indicating treatment efficacy. (B) Cells were treated with vehicle control, 2.5, or 5, 10 μM verteporfin for 3 days and MTT assay was performed to assess cell viability. Verteporfin is cytotoxic...

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