88,327 Works

Different subcellular localizations and functions of Arabidopsis myosin VIII-4

Lior Golomb, Mohamad Abu-Abied, Eduard Belausov & Einat Sadot
ATM1(IQ-tail) (dots of 980 ± 145 nm in diameter). B. FM4-64. C. Overlay of A and B (1 optic section). D. GFP-ATM1(IQ-tail) (dots of 630 ± 60 nm). E. FYVE-DsRED. F. Overlay of D and E (1 optic section). G. RFP-ATM1(IQ-tail) (dots of 300 ± 100, colored green for ease of demonstration). H. GFP-ARA7 (colored magenta for ease of demonstration). I. Overlay of G and H (1 optic section). J. RFP-ATM1(IQ-tail) (dots of 570 ±...

Rice Yellow Mottle Virus stress responsive genes from susceptible and tolerant rice genotypes-4

Marjolaine Ventelon-Debout, Christine Tranchant-Dubreuil, , Martine Bangratz, Christelle Siré, Michel Delseny & Christophe Brugidou
were loaded with: reaction products from RNAs of IR64 infected leaves harvested at 2, 5, and 7 dpi, RNAs of Azucena infected leaves harvested at 3, 5, and 7 dpi, 1 kb ladder (Promega).Copyright information:Taken from "Rice Yellow Mottle Virus stress responsive genes from susceptible and tolerant rice genotypes"http://www.biomedcentral.com/1471-2229/8/26BMC Plant Biology 2008;8():26-26.Published online 3 Mar 2008PMCID:PMC2275266.

Technical validation of microarray data by quantitative RT-PCR (QRT-PCR)

Tim Demuth, Jessica L Rennert, Dominique B Hoelzinger, Linsey B Reavie, Mitsutoshi Nakada, Christian Beaudry, Satoko Nakada, Eric M Anderson, Amanda N Henrichs, Wendy S McDonough, David Holz, Anna Joy, Richard Lin, Kuang H Pan, , Stan N Cohen & Michael E Berens
Average Log2 expression of relative mRNA copy numbers derived from three replicate microarray experiments and QRT-PCR of migratory (rim) over stationary (core) glioma cells (error bars = SD). Concordance between directionality of differential regulation between migratory and stationary cells for microarray and QRT-PCR data is displayed in parentheses (CYR61 = cystein rich 61, CTGF = connective tissue growth factor, RRAS2 = related RAS viral oncogene homolog 2, RhoA = ras homolog gene family member A,...

Derived from SAM analysis of union of and ; fast migrating cell lines colored in green, slow migrating cell lines colored in red ()

Tim Demuth, Jessica L Rennert, Dominique B Hoelzinger, Linsey B Reavie, Mitsutoshi Nakada, Christian Beaudry, Satoko Nakada, Eric M Anderson, Amanda N Henrichs, Wendy S McDonough, David Holz, Anna Joy, Richard Lin, Kuang H Pan, , Stan N Cohen & Michael E Berens
K-means (k = 3) identified three populations in malignant astrocytoma samples stratified by age that were visualized by multidimensional scaling (MDS); young (< median age) () and old patients (> median age) (). Kaplan-Meier survival analysis for clusters derived from k-means stratified by age; young (), old () show significant differences in overall survival.Copyright information:Taken from "Glioma cells on the run – the migratory transcriptome of 10 human glioma cell lines"http://www.biomedcentral.com/1471-2164/9/54BMC Genomics 2008;9():54-54.Published online 29...

Neoplastic transformation of rat liver epithelial cells is enhanced by non-transferrin-bound iron-2

Donald J Messner & Kris V Kowdley
Itional 16 weeks in four separate experiments. Media additions during the promotion phase and the number of replicates were: 200 μM ammonium citrate (AmCit), n = 4 (- MNNG), n = 4 (+ MNNG); 200 μM ferric ammonium citrate (FAC), n = 3 (- MNNG), n = 4 (+ MNNG); or 200 μM ferric ammonium citrate,160 μM desferoxamine (FAC, dfo), n = 2 (+ MNNG). Cell transformation was assayed by growth in soft agar as...

C6 glioma cells were incubated for 24 h with 10 μM berberine (Ber) or 5 μM AsO(As), and with 10 μM berberine and 5 μM AsO(A Ber)

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Total cell lysates were prepared and subjected to Western blot analysis. Protein levels of phosphorylated ERK1/ERK2 and non-phosphorylated ERK1/ERK2, and ODC, MT1-MMP and MMP2 were detected using the respective monoclonal antibodies (A). The condition serum free media were collected for gelatin zymography analysis from C6 glioma and U-87 cells. Determined activities of these proteins were subsequently quantified by densitometric analysis with the value of controls set at 100% as shown in the graph. The quantitative...

Seasonality of cases of malaria (severe and uncomplicated) admitted to the hospital and rainfall pattern

Quique Bassat, Caterina Guinovart, , Pedro Aide, , , Azucena Bardají, Ariel Nhacolo, Eusébio Macete, Inácio Mandomando, John J Aponte, Clara Menendez & Pedro L Alonso
Copyright information:Taken from "Malaria in rural Mozambique. Part II: children admitted to hospital"http://www.malariajournal.com/content/7/1/37Malaria Journal 2008;7():37-37.Published online 26 Feb 2008PMCID:PMC2275288.

Peak plantar pressure values for taped and untaped conditions for each region of the foot; medial forefoot (MFF), lateral forefoot (LFF), medial midfoot (MMF), lateral midfoot (LMF), medial hindfoot (MHF) and lateral hindfoot (LHF)

Kieran O'Sullivan, , Emer O'Neill & Una Ni Mhainin
These differences were statistically significant for the lateral midfoot (p = 0.000), the medial forefoot (p = 0.014), and the medial (p = 0.000) and lateral (p = 0.007) hindfoot.Copyright information:Taken from "The effect of low-dye taping on rearfoot motion and plantar pressure during the stance phase of gait"http://www.biomedcentral.com/1471-2474/9/111BMC Musculoskeletal Disorders 2008;9():111-111.Published online 18 Aug 2008PMCID:PMC2529302.

Unique motifs identify PIG-A proteins from glycosyltransferases of the GT4 family-5

Nupur Oswal, Narinder Singh Sahni, Alok Bhattacharya, Sneha Sudha Komath & Rohini Muthuswami
Glycosyltransferase proteins from prokaryotes, archaeabacteria, and eukaryotes.Copyright information:Taken from "Unique motifs identify PIG-A proteins from glycosyltransferases of the GT4 family"http://www.biomedcentral.com/1471-2148/8/168BMC Evolutionary Biology 2008;8():168-168.Published online 4 Jun 2008PMCID:PMC2446393.

Effect of sample size and varying attributable risk at the linked locus on estimated power

Richard A Kerber, Christopher I Amos, , Dianne M Finkelstein & Duncan C Thomas
Copyright information:Taken from "Design considerations in a sib-pair study of linkage for susceptibility loci in cancer"http://www.biomedcentral.com/1471-2350/9/64BMC Medical Genetics 2008;9():64-64.Published online 10 Jul 2008PMCID:PMC2488325.

Tapping mode atomic force microscope amplitude topography of the three DNA molecules complexed with various gene carriers

Charlie Yu Ming Hsu & Hasan Uludağ
All images were captured at a scan size of 6 × 6 μm, except where noted. c-DNA without carriers [8 × 8 μm] (), with PEI (), PLL (), PLL-PA (), and Lipofectamine-2000™ (). l-DNA without carriers (), with PEI (), PLL (), PLL-PA (), and Lipofectamine2000™ (). pcr-DNA without carriers [5 × 5 μm] (), with PEI (), PLL (), PLL-PA (), and Lipofectamine-2000™ ().Copyright information:Taken from "Effects of size and topology of DNA...

Simplified approaches for the development of an ELISA to detect circulating autoantibodies to p53 in cancer patients-2

, , , , , &
O nickel coated microtiter plate (B) were subjected to react with various amounts of anti-p53 mAb (0, 1:10000, 1:5000 and 1:1000 dilution) and anti-histidine mAb (1:1000 dilution). The p53-BCCP fusion protein directly immobilized onto avidin-coated microtiter plate were reacted with various amounts of anti-p53 mAb (0, 1:10,000, 1:5,000 and 1:1,000 dilution) and anti-biotin mAb (1:1000 dilution). Experiment was performed in triplicate and error bars represent standard deviation.Copyright information:Taken from "Simplified approaches for the development of...

Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET-3

Catarina Madeira, Luís MS Loura, Manuel Prieto, Aleksander Fedorov &
Tris-HCl, pH 7.4 (-○-); Lipoplexes prepared in PBS (-◆-); Lipoplexes prepared in Tris-HCl and incubated on PBS (-△-); Lipoplexes prepared in Tris-HCl, incubated on PBS, followed by incubation on FBS (-□-).Copyright information:Taken from "Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET"http://www.biomedcentral.com/1472-6750/8/20BMC Biotechnology 2008;8():20-20.Published online 26 Feb 2008PMCID:PMC2275333.

Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides-3

Holden T Maecker, Jeffrey Hassler, Janice K Payne, Amanda Summers, Karrie Comatas, , Michael A Morse, Timothy M Clay, Herbert K Lyerly, Sonny Bhatia, Smita A Ghanekar, Vernon C Maino, Corazon DelaRosa & Mary L Disis
Onder. The same non-responding donor was used for all assays. Unstimulated background was subtracted for each dilution point in CFC and ELISPOT assays. This background was uniformly low (< 0.08% for CFC and < 15 SFC per 2.5 × 10PBMC for ELISPOT) despite the used of allogeneic PBMC for the dilution. Note that the pp65peptide response of this donor (#43) was much lower in ELISPOT compared to the other two assays.Copyright information:Taken from "Precision and...

Abnormal skeletal and cardiac development, cardiomyopathy, muscle atrophy and cataracts in mice with a targeted disruption of the gene-1

Emma Heath, Dalal Tahri, Elisabetta Andermarcher, Paul Schofield, Stewart Fleming & Catherine A Boulter
Arsal cartilage elements (white arrow) and elongation of the digits in -/-. Note increased intensity of Alizarin red staining and thickening of the tibia in -/- compared to wild type. C. Knee abnormalities in -/- compared with wild type, including flattening of the patella. D. Malformation of the wrist elements in -/- compared to wild type. E. Dislocation of hip (arrow) in -/- compared to wild type. F. Kinking of tail with compression of the...

The localization of filamin and α-actinin derived probes in non-polarized cells

Raymond W Washington & David A Knecht
(A and B) When expressed in vegetative cells the localization pattern of GFP-FLNABD (B) was similar to that of GFP-FLN (A). Both probes localized strongly to the peripheral cortex, the leading edge of motile cells (arrowheads), new pseudopods (arrows) and macropinocytic cups (*). (C and D) When expressed in vegetative cells both GFPα-A (C) and GFP-αAABD (D) are present in new protrusions (arrows) and at the leading edge of motile cells (arrowheads) and to macropinocytotic...

Role of a NLS in determining the cellular distribution of RASSF2A and apoptosis

, , Minoru Toyota, , Takashi Imai, Mutsumi Ohe-Toyota, Eiichiro Yamamoto, Masanori Nojima, Tomoko Fujikane, Yasushi Sasaki, Toshiharu Yamashita, Yoshiyuki Watanabe, , Koichi Hirata, Fumio Itoh, , Yasuhisa Shinomura & Takashi Tokino
() Cellular distribution of RASSF1 and RASSF2A. () Apoptosis induced by the indicated RASSF2A mutants. Cells were harvested, fixed in methanol and stained with propidium iodide 72 h after infection with the respective adenoviral vector. Percentages of apoptotic cells are shown on the -axis. Various adenoviral vectors were infected at a multiplicity of infection of 100. Lac-Z: Ad-LacZ; F2: Ad- ΔN: Ad--ΔN; ΔRA: Ad--ΔRA; ΔNLS: Ad--ΔNLS.Copyright information:Taken from "Cytoplasmic RASSF2A is a proapoptotic mediator whose...

The effectiveness of pulsed electrical stimulation (E-PES) in the management of osteoarthritis of the knee: a protocol for a randomised controlled trial-2

Robyn E Fary, Graeme J Carroll, Tom G Briffa, Ritu Gupta &
-36 – Medical Outcomes Study 36-item Short-Form Survey; HAP – Human Activity Profile; GPES – Global Perceived Effectiveness Scale.Copyright information:Taken from "The effectiveness of pulsed electrical stimulation (E-PES) in the management of osteoarthritis of the knee: a protocol for a randomised controlled trial"http://www.biomedcentral.com/1471-2474/9/18BMC Musculoskeletal Disorders 2008;9():18-18.Published online 4 Feb 2008PMCID:PMC2275728.

Size distribution of paralogous protein families in rice and Arabidopsis

, Shu Ouyang, Amy Egan, Kan Nobuta, Brian J Haas, Wei Zhu, Xun Gu, Joana C Silva, Blake C Meyers &
The exact number of families is listed above the bars.Copyright information:Taken from "Characterization of paralogous protein families in rice"http://www.biomedcentral.com/1471-2229/8/18BMC Plant Biology 2008;8():18-18.Published online 19 Feb 2008PMCID:PMC2275729.

Immunoblot showing levels of wild-type and mutant TSC1-FLAG proteins and endogenous TSC2 in 97-1 cell lines

Louis S. Pymar, Fiona M. Platt, Jon M. Askham, Ewan E. Morrison & Margaret A. Knowles
() Measurement of TSC1 RNA levels by real time RT–PCR analysis of wild-type and mutant TSC1-FLAG mRNA transcript levels in 97-1 cell lines. RT−ve is reverse transcriptase-negative control, NTC is no template control. TSC1 expression is standardized to SDHA and normalized to the 97-1 Neo cell line.Copyright information:Taken from "Bladder tumour-derived somatic missense mutations cause loss of function via distinct mechanisms"Human Molecular Genetics 2008;17(13):2006-2017.Published online 7 Apr 2008PMCID:PMC2427143.© The Author 2008

Derived from inclusions late in the infectious cycle induce aponecrosis in human aortic endothelial cells-5

Joseph Marino, Isabelle Stoeckli, Michael Walch, Sonja Latinovic-Golic, Hanna Sundstroem, Peter Groscurth, Urs Ziegler & Claudia Dumrese
Ols were additionally treated with chloramphenicol. Cells were stained with anti C. pneumoniae-MOMP (red), D3521 (green), DAPI (blue) and colocalization of D3521 and MOMP was calculated (white). A: Infected HAEC 72 hpi contain a MOMP positive inclusion (asterisk). B: Spot-like infected healthy HAEC 48 hpi display a number of MOMP positive spots (arrows) of which only very few colocalize with the ceramide signal. Hence, 24 h after ceramide supply D3521 colocalizes with the MOMP signal...

Structural basis of GC-1 selectivity for thyroid hormone receptor isoforms-0

Lucas Bleicher, Ricardo Aparicio, Fabio M Nunes, Leandro Martínez, Sandra M Gomes Dias, Ana Carolina Migliorini Figueira, Maria Auxiliadora Morim Santos, Walter H Venturelli, Rosangela Da Silva, Paulo Marcos Donate, Francisco AR Neves, , John D Baxter, Paul Webb, Munir S Skaf & Igor Polikarpov
red, gray and blue correspond to regions I, II and III described in the text. Apolar residues are shown in gray, basic residues in blue (namely His435, Arg316, Arg282 and Arg320) and polar residues in orange (including Asn331).Copyright information:Taken from "Structural basis of GC-1 selectivity for thyroid hormone receptor isoforms"http://www.biomedcentral.com/1472-6807/8/8BMC Structural Biology 2008;8():8-8.Published online 31 Jan 2008PMCID:PMC2275733.

Structural basis of GC-1 selectivity for thyroid hormone receptor isoforms-1

Lucas Bleicher, Ricardo Aparicio, Fabio M Nunes, Leandro Martínez, Sandra M Gomes Dias, Ana Carolina Migliorini Figueira, Maria Auxiliadora Morim Santos, Walter H Venturelli, Rosangela Da Silva, Paulo Marcos Donate, Francisco AR Neves, , John D Baxter, Paul Webb, Munir S Skaf & Igor Polikarpov
En) and hTRβ (magenta). All interactions are maintained between the ligand and the binding site residues in both hTR isoforms. Both Ser277 and Asn331 interact with the amino group of Tthrough their amide nitrogen, leading to similar conformations of these residues. GC1 bound to TRα: multiple conformations of the Arg228 are observed. In the productive conformation there is a strong interaction with the ligand (cyan), while in non-productive conformations this residue interacts with the side-chain...

Glial fibrillary astrocytic protein (GFAP) and βIII-tubulin expression in differentiated olfactory bulb-derived (OB-ns) and subventricular zone-derived (SVZ-ns) neurospheres

Perrine Barraud, , Maeve A Caldwell & Robin JM Franklin
(A-B) Photomicrographs showing GFAP-expression in control OB-ns (A) and OM-CM cultures (B). Proportions of GFAP-expressing cells in OB-ns and SVZ-ns cultures are shown in histogram graphs in C and D, respectively. (E-F) Photomicrographs showing βIII-tubulin expression in control (E) and OM-CM cultures (F). Proportions of βIII-tubulin-expressing cells in OB-ns and SVZ-ns cultures are shown in histogram graphs in G and H, respectively. Scale bar in A, B, E, F: 50 μm.Copyright information:Taken from "Secreted factors...

Addition of the microchromosome GGA25 to the chicken genome sequence assembly through radiation hybrid and genetic mapping-3

Marine Douaud, Katia Fève, Marie Gerus, Valérie Fillon, Suzanne Bardes, David Gourichon, Deborah A Dawson, Olivier Hanotte, Terry Burke, Florence Vignoles, Mireille Morisson, Michele Tixier-Boichard, Alain Vignal & Frédérique Pitel
Gion 144.1–159.5 Mb [37]. For each marker on the framework map, a line joins both positions (cR and Mb) together. Framework markers are in red. Left: conservation of synteny between HSA1 and chicken chromosomes. Pink: GGA25, blue: GGA08, orange: GGA01.Copyright information:Taken from "Addition of the microchromosome GGA25 to the chicken genome sequence assembly through radiation hybrid and genetic mapping"http://www.biomedcentral.com/1471-2164/9/129BMC Genomics 2008;9():129-129.Published online 17 Mar 2008PMCID:PMC2275740.

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