688 Works

Clonality assays in adrenal medullary and thyroid C-cell proliferation

Salvador J. Diaz-Cano
This review covers the role of RET protooncogene in the pathogenesis of thyroid C-cell and adrenal medullary cells proliferative lesions and the clonal and genetic evolution of these lesions, especially emphasizing the role of other genes. These key elements contribute to the molecular progression and intralesion heterogeneity of such lesions.


Transferu Technologii I Rozwoju Fundacja Uniwersytetu Śląskiego Centrum Innowacji
"Preferencje polityczne" to pismo o profilu humanistycznym, prezentujące na swoich kartach interdyscyplinarne podejście do problematyki zachowań wyborczych, szczególnie czynników determinujących identyfikacje polityczne. Pismo jest otwarte na współpracę z różnymi ośrodkami naukowymi w kraju. Wydawcą jest Centrum Innowacji, Transferu Technologii i Rozwoju Fundacja Uniwersytetu Śląskiego. Patronat nad projektem sprawuje Polskie Towarzystwo Nauk Politycznych.

Introduction to apoptosis

Salvador J. Diaz-Cano &
Kerr and colleagues (II) coined the term" apoptosis" to designate a particular form of cell death distinct from necrosis. The salient features of apoptosis, which Kerr defined in histopathologic terms, were the breakup of individual cells into smaller, often round or ovoid cytoplasmic fragments containing pyknotic nuclear debris, called" apoptotic bodies," which were subsequently phagocytized, usually by adjacent cells. This minireview focuses the attention of main molecular pathways of apototis, as default cellular mechanism to...

Clonality: Molecular criteria and clinical utility

Salvador J. Diaz-Cano &
Conceptually, a clone is a group of genetically identical cells derived from a single common ancestor Most somatic tissues arc polyclonal, unless there is a significant growth advantage within a subset of cells which proliferate at the expense of surrounding cells. Clonal proliferation in most somatic tissues may be viewed as evidence of the creation of a cell or group of cells with a growth advantage Clonal derivation of cells is a hallmark of neoplasia,...

PCR-based alternative for diagnosis of immunoglobulin heavy chain gene rearrangement: principles, practice, and polemics

Salvador J. Diaz-Cano
PCR-based detection of immunoglobulin gene rearrangements is a reliable and accurate method which can be utilized as a clinical test if appropriate quality controls are performed (internal, polyclonal and monoclonal, sensitivity and specificity controls) and strict criteria for clonal rearrangements are used to exclude false-positives and false-negatives.

Cellular blue nevus with massive regional lymph node metastases

Salvador J. Diaz-Cano, , , , &
Small, well-differentiated groups of nevus cells have been found occasionally in the marginal sinuses and parenchyma of regional lymph nodes that drain sites of cellular blue nevi.
The histologic, immunohistochemical, and karyometric description of a pigmented cutaneous lesion, with the features of cellular blue nevus and located on the leg of a 14-year-old woman, that was accompanied by synchronic presentation of massive inguinal lymph-node metastases.
The excised specimens were processed routinely, embedded in paraffin, and...

Motesanib diphosphate in progressive differentiated thyroid cancer

Salvador J. Diaz-Cano
Sherman et al. (July 3 issue)1 highlight the importance of selecting patients for alternative therapies, such as motesanib diphosphate, in the treatment of progressive differentiated thyroid carcinomas. Although radiographic indings are useful criteria for predicting a response to motesanib, they can be supplemented by the microscopical findings — in particular, growth pattern, necrosis, and desmoplastic reaction — which have been correlated with progression prediction.2 The genotypic alterations described in follicular-cell neoplasms control the mitogen-activated protein...


, , & Salvador J. Diaz-Cano
Case Report: A 36-year-old presented with abdominal cramps, diarrhoea and several episodes of profuse rectal bleeding (1 week duration). No associated fever sweats or weight loss. He was haemodynamically stable. He had mild left iliac fossa tenderness, no organomegaly and bowel sounds were normal. Digital rectal examination revealed some fresh blood but no haemorrhoids and a normal prostrate. Other systems were unremarkable. Investigations: c-RP 10 mg/l, Hb 13.5 g/dl, WBC 8.34×103/mm3, and platelets 162×103/mm3. Multiple...

Molecular evolution and intratumor heterogeneity by topographic compartments in muscle-invasive transitional cell carcinoma of the urinary bladder

Salvador J. Diaz-Cano, Alfredo Blanes, , , &
Superficial transitional cell carcinomas (TCC) of the urinary bladder have been shown to be monoclonal. However, no combined study of clonality and tumor suppressor genes (TSG) is available to date for muscle-invasive TCC. Forty-four muscle-invasive TCC of the urinary bladder selected from women were included in this study. Tumor cells located above and below the muscularis mucosa zone were systematically microdissected and used for DNA extraction. Hha-I digested and undigested samples were used to study...

Analysis of clonality of atypical cutaneous lymphoid infiltrates associated with drug therapy by PCR/DGGE

Salvador J. Diaz-Cano, , &
Atypical lymphocytic infiltrates that mimic cutaneous lymphoma (ie, pseudolymphoma) are often observed in skin biopsy specimens from patients with altered immune function. The latter may reflect systemic immune dysregulatory states such as collagen vascular disease or human immunodeficiency virus infection. Among the iatrogenic causes are drug therapy with agents that abrogate lymphocyte function. These drugs encompass the anticonvulsants, antidepressants, phenothiazines, calcium channel blockers, and angiotensin-converting enzyme inhibitors. The appellation of lymphomatoid hypersensitivity reaction has been...

Trichogerminoma: further evidence to support a specific follicular neoplasm

Salvador J. Diaz-Cano &
A 74-year-old woman had developed a slowly growing lesion on the face over a period of 18 months, which became ulcerated and was excised with a clinical diagnosis of basal cell carcinoma. Histological examination revealed a well-circum- scribed lesion invading the deep dermis, not connected with the superficial epidermis, and separated from the surrounding soft tissue by a thick capsule (Figure 1A). Perineural invasion but no vascular or lymphatic invasion was demonstrated at the periphery...

Urbanization, ecosystems, and biodiversity: Assessments of India and Bangalore

, , Madhusudan Katti, Maria Tengö & Maria Schewenius
This publication is an outcome and part of the global Cities and Biodiversity Outlook (CBO). The CBO project provides a global assessment in two publications, of the links between urbanization, biodiversity, and ecosystem services. The first part, Action and Policy, is released in Hyderabad at the Cities for Life Summit, parallel to the eleventh meeting of the Conference of the Parties to the Convention on Biological Diversity (CBD) in October 2012. Core chapters of the...

CoE 46: Tree (Structures) of Life

Bradly Alicea
Carnival of Evolution (blog carnival) #46: Tree (Structures) of Life. A review of evolution-related blog posts, papers, and other relevant blog content for the month of April, 2012. Originally presented on April, 1, 2012 at the Synthetic Daisies blog: http://syntheticdaisies.blogspot.com/2012/04/carnival-of-evolution-number-46-tree.html

Quantum perturbation theory in stock trading

It is a hot topic about how to trade a stock/group of stocks in a non-news day. Author tries to design safe, profitable automated stock trading agents using evolutionary algorithms[2].

[Teaching Program on Anatomic and Molecular Pathology] [Spanish]

Salvador J. Diaz-Cano
Teaching program on anatomical and molecular pathology that includes both teaching techniques and content (general and specific objectives). Brief historic perspective and evaluation of research assessment are also included. Key words: teaching, anatomic pathology, molecular pathology, program, examination, medicine, pedagogy and education, open competitive examination.

Histologic criteria for adrenocortical proliferative lesions: value of mitotic figure variability

Salvador J. Diaz-Cano & Alfredo Blanes
This study compared 3 systems and a newly designed stepwise discriminant diagnostic system (SDDS) to assess accuracy, reproducibility, and reliability in adrenocortical nodular hyperplasia (ACNH; n = 82), adenoma (ACA; n = 78), and carcinoma (ACC; n = 32) (diagnoses according to World Health Organization criteria; median follow-up, 135 months). In cross-validations, we studied cortex appearance, growth pattern, cytoplasmic features, nuclear parameters, mitotic figure counting (MFC), necrosis, invasion, and stromal-inflammatory reactions. The SDDS independent predictors...

Cytoarchitectural and kinetic features in the histological evaluation of follicular thyroid neoplasms

Salvador J. Diaz-Cano, Alfredo Blanes, &
The diagnosis of follicular thyroid carcinomas is mainly based on capsular and vascular invasion. The aim of this study was to determine the diagnostic relevance of nuclear features, inflammation and stromal changes.
Anisokaryosis, chromatin pattern, nucleolus, nuclear pleomorphism, nuclear/cytoplasmic ratio, necrosis, stromal changes and tumour interstitial lymphocytes (TIL) were analysed in adenomatous hyperplastic nodules (39), adenomas (43) and carcinomas (28 minimally invasive, 48 widely invasive and 27 anaplastic). Ki67 immunostaining, in situ end labelling (ISEL) for...

Tiered 'authorship' as a simple, quantifiable and greyscale measure of contribution?

Aidan Keith
Comment on the possible benefits of a tiered 'authorship' system in science. This paper is based on a blog post on 6th November 2012.

Hart and Chamberlain NCEAS proposal

Edmund Hart & Scott Chamberlain
This is our unfunded NCEAS proposal from September 2012 about climate change, adaptation and open data.

Cymbalaria muralis P. Gaertn., B. Mey. & Schreb. and Cymbalarietum muralis Görs 1966 in Lower Silesia - expansion or regression? - Phytogeographical problems of synanthropic plants: 185-193, 2003

Krzysztof Świerkosz & Ewa Szczęśniak
Cymbalaria muralis, a species native in Southern Alps, Western Yugoslavia, Italy and Sicily, became popular in Central Europe in 19th century as an ornament plant. The first time the species was noticed in Lower Silesia in Karpniki village in 1841 (WIMMER 1841), and number of known sites increased to 67 in 1945. After 1945 21 new localities were found, but about 40% of all known data was not confirmed in 1991-1999. Analysis supports the hypothesis...

Molecular mechanisms in melanoma

Salvador J. Diaz-Cano
The review of melanoma by Mil- ler and Mihm (July 6 issue)1 clearly characterizes the linear progression of melanocytic lesions, from the morphologic perspective to the molecular per- spective.2,3 However, the genetic alterations would have more relevance if they result in kinetic ad- vantage and progression. My colleagues and I have studied a series of dysplastic nevi (92 low-grade and 31 high-grade lesions) and melanomas in situ (15 lesions) using proliferation (Ki-67 labeling), apoptosis (in...

Krasovsky K (2012) Tobacco taxation policy in three Baltic countries after the EU accession

Tatiana Andreeva & Konstantin Krasovsky
Tobacco taxation policy in three Baltic countries after the EU accession
Konstantin Krasovsky BACKGROUND: Estonia, Latvia and Lithuania joined the EU in 2004 and had to increase tobacco excise rates. The aim of the paper is to explore the impact of tax policies on tobacco consumption, revenue and tobacco market in the Baltic countries.
METHODS: Data on tobacco sales, tax rates, prices, revenues, and smoking prevalence were taken from databases and reports. Tobacco affordability index...

Deep dermal up-regulation of HIF-1A is directly correlated with apoptosis in Spitz tumors

, , & Salvador J. Diaz-Cano
Background: The microvessel profile bases in cutaneous atypical melanocytic lesions (atypical melanocytic nevi, AMN, and Spitz tumors, ST) are poorly understood. No study has correlated microvessel profile and HIF-1 expression with cell kinetics by topographic compartments in AMN and ST to date. Design: We selected low-grade AMN (93), high-grade AMN (31), ST (42), malignant melanomas (42, 15 radial growth phase MM-RGP and 27 vertical growth phase MM- VGP), and conventional melanocytic nevi (15 junctional, 20...

Germline RET 634 mutation positive MEN 2A-related C-cell hyperplasias have genetic features consistent with intraepithelial neoplasia

Salvador J. Diaz-Cano, Alfredo Blanes, , &
C-cell hyperplasias are normally multifocal in multiple endocrine neoplasia type 2A. We compared clonality, microsatellite pattern of tumor suppressor genes, and cellular kinetics of C-cell hyperplasia foci in each thyroid lobe. We selected 11 females from multiple endocrine neoplasia type 2A kindred treated with thyroidectomy due to hypercalcitoninemia. C-cell hyperplasia foci were microdissected for DNA extraction to analyze the methylation pattern of androgen receptor alleles and microsatellite regions (TP53, RB1, WT1, and NF1). Consecutive sections...

Molecular markers of apoptosis in breast carcinomas

& Salvador J. Diaz-Cano
Apoptosis is a distinct mode of programmed cell death that plays an important role in cellular kinetics in both normal and pathologic conditions, including neoplastic and precancerous lesions, viral pathogenesis (eg, acquired immunodeficiency syndrome), inflammatory and immune responses and neurodegenerative disorders, among others (Arends and Wyilie, 1991.1 Berges and lsaacs, 1993" Steller, 1995.1 Thompson, 1995). It is associated with numerous aspects of mammalian embryogenesis, especially in proliferating somatic tissues (Evan et al, 1995; Salomon and...

Registration Year

  • 2012

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