Supplementary material. Autologous iPSC-derived four-organ-chip
Microphysiological systems play a pivotal role in progressing towards a global paradigm shift in drug development. Here, we designed a four-organ-chip interconnecting miniaturized human intestine, liver, brain and kidney equivalents. All four organ models were pre-differentiated from induced pluripotent stem cells from the same healthy donor and integrated into the microphysiological system. The co-culture of the four autologous tissue models in one common medium deprived of tissue specific growth factors was...

Supplementary Figure S1. Plasmid map of pMYU1ATED. pMGT (truncated) indicates a fragment of 2576 bp from position 18 to 2593 of pMGT (GenBank accession number NC_007706). The TED module is separated by MCS2 between mlcR25 (magenta) and the lac operator (not labeled) to mimic the plasmid used in the original work (1).

Example Assay Definition File

Supplementary Table 2 Amount of Operational Taxonomic Units (OTUs) before and after filtering. For the mock libraries (A, B, and C). OTUs that were chimeric were removed, and OTUs that matched ≤95% to one of the mock community members were considered to be a weak consensus match and were also removed.

Supplementary Table 1 The amount and percentage of contaminant sequences in the negative control that passed through quality filtering and clustered into operational taxonomic units (OTUs). The negative control contained trace contaminants from nine of the 16 mock community members. This is presumably due to cross-contamination during PCR and library preparation steps, despite surface sterilization and stringency in protocols.

Supplemental Figure. 3. Quantifying the signal of single PLA for A2AR and D2R, and dual PLA for D2R-A2AR, in the NAcc. The numbers of PLA puncta / mm2 were quantified by BOPSS (A-D, data were plotted as mean ± SEM). The fractions of D2R-A2AR dual PLA puncta relative to D2R (E) and A2AR (F) single PLA were calculated with the means in (A-C) and data were plotted as mean ± propagated error. The error was...

Supplemental Table 1. Spearman rank order correlations between individual analysis methods. Significant (p<0.05) coefficients (rs) are indicated in bold. Supplemental Table 2. Impact of amount of starting DNA on telomere lengths (n=3) calculated using 4 different analysis methods and applying 2 different equations.

Supplemental Methods and Materials Supplemental Table 1. Human Subject information Supplemental Table 2. ROI, sampling areas and counting areas

Supplemental file 1 for: An efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture Supplementary Figure 1: Plasmodium falciparumDNA from spiked whole blood determined by PfBetatubulin PCR. A and B. low volume eluate template, 1 μl C and D. high volume eluate template, 10 μl. Two extractions run per parasitaemia condition. M: 100bp marker, NTC: No template control. Percentage denotes the parasitaemia post spiking. Supplementary Figure 2: Plasmodium...

Supplemental file 1 for: An efficient and novel technology for the extraction of parasite genomic DNA from whole blood or culture Supplementary Figure 1: Plasmodium falciparumDNA from spiked whole blood determined by PfBetatubulin PCR. A and B. low volume eluate template, 1 μl C and D. high volume eluate template, 10 μl. Two extractions run per parasitaemia condition. M: 100bp marker, NTC: No template control. Percentage denotes the parasitaemia post spiking. Supplementary Figure 2: Plasmodium...

Supplemental Figure 1. Bright-field image of B. cereus cells taken from a stationary phase culture and incubated in a 0.4% EB solution. In two instances EB specifically stains a single compartment in a filamentous chain.

Supplementary Figure S1. Improvement of PCR reaction system using dilution comparing to spin column. M: 100bp marker. (A) The patterns of the first/second-round PCR reaction system by agarose gel electrophoresis. For the 50μL PCR reaction system, compared with the previously reported primer concentration which was 3.3μL (ITSP1s) and 0.66μL (AP1) in Lane l, Lane 2 shows no difference in the yield when primer concentration was 2.2μL (ITSP1s) and0.44μL (AP1). For the 10μL PCR reaction system,...

Supplementary Table 1 - List of assays used for qPCR analysis of candidate reference genes. Information on RPLP0 reference gene analysis are reported within the text. Supplementary Table 2 – List of primers used for qPCR analysis of circular RNA for validation of hybridization array results. The primers were used for qPCR detection of circular RNAs with a SYBR green chemistry. Supplementary Table 3 – List of assays used for qPCR analysis of microRNA (miRNA)...

Supplementary Table 1 - List of assays used for qPCR analysis of candidate reference genes. Information on RPLP0 reference gene analysis are reported within the text. Supplementary Table 2 – List of primers used for qPCR analysis of circular RNA for validation of hybridization array results. The primers were used for qPCR detection of circular RNAs with a SYBR green chemistry. Supplementary Table 3 – List of assays used for qPCR analysis of microRNA (miRNA)...

PROTOCOL FOR: Optimized transformation, overexpression and purification of S100A10

Supplemental Table 1 - Selected candidate reference genes evaluated in this study and the information of the primers used for RT-qPCR.

Supplementary Figure 2. Target inserting the IL-37 into AASV1 site. (A) Donor and gRNA structures. (B) PCR characterization of genome edited MSC colonies by amplifying the left arm. Arrow shows the predicted PCR product size. (C) PCR characterization of genome edited MSC colonies by amplifying the right arm. Arrow shows the predicted PCR product size. (D) mRNA level of IL-37 among the colony 8 (C8) without donor insertion, colony 20 (C20) with correct insertion, and...

Figure S3. As-synthesized GO (left) and rGO (right) solutions.

Figure S2. The effect of ssDNA-Au electrode treated with TMPD only.Figure S2 shows the CV curves of [Fe(CN)6]3−/[Fe(CN)6]4− recorded using ssDNA-Au electrode after treatment with 1 mM TMPD only at various time points. The nearly identical current responses over time showed that TMPD itself has little effect on [Fe(CN)6]3−/[Fe(CN)6]4− signal recovery.

Figure S2. The effect of ssDNA-Au electrode treated with TMPD only.Figure S2 shows the CV curves of [Fe(CN)6]3−/[Fe(CN)6]4− recorded using ssDNA-Au electrode after treatment with 1 mM TMPD only at various time points. The nearly identical current responses over time showed that TMPD itself has little effect on [Fe(CN)6]3−/[Fe(CN)6]4− signal recovery.

To analyze DNA molecules, it is important that little contaminating DNA is present so that it does not drown out the signal of the DNA you are interested in. This paper describes a simple method of filtering out small DNA molecules so that only the large, interesting molecules are left behind. We do so by using filters that have holes of a very precisely defined size so that only DNA smaller than these holes can...

Supplementary Figure S1. Plots and fits of the average loss of DNA in pmol over time for all sizes of DNA.

Supplementary Figure S3. Agarose gel stained with Sybr Gold with multiple 20 and 40 hour dialysis samples of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S2.

Supplementary Figure 1

Supplementary material