I study GATA2 and GATA3 role in trophoblast development and hematopoiesis development. I combine Crispr Cas9 for double knockout both GATA2 and GATA3. GATA2&3 DKO cell was characterized with IF stianing, WB. Then human TSC differentiation was performed. For hematopoiesis development, i conducte HEMO to test hematopoiesis differentiation efficiency of GATA2&3 DKO cells.

This file contains all raw and processed data of the thesis titled 'Nsp1 facilitates SARS-CoV-2 replication through calcium- calcineurin-NFATc2 signaling pathway'. The research study investigated how the SARS-CoV-2-encoded viral protein nsp1 hijacked the cellular calcieurin-NFAT pathway to substantiate SARS-CoV-2 replication and explored possible anti-nsp1 strategy to mitigate SARS-CoV-2 viral replication. This encrypted file contains the nanopore sequencing data for DelNSP1 BACmid and virus (Folder 1), the raw data for all infection assays, qPCR and luciferase...

The dataset includes all of the raw data involved in the PhD thesis. Each categorized data has been combined in a separate folder. A detailed description of the individual file has been involved in the README file.

Our society is facing increasing burden of health care associated with cancer disease. At present, there are more than hundreds of anti-cancer drugs. At the same time, the number of compositions made from these clinically approved anti-cancer drugs is up to 1300-1500.[1] Among the clinically used anti-cancer drugs, cytotoxic platinum (II) compounds are the most extensively used in chemotherapy. In this project, the platinum(II) complexes bearing different bis-N-heterocyclic carbene and 1- (3-hydroxybenzo[b]thiophen-2-yl)ethanone (HO^O) ligand were...

This file contains results of histology, confocal microscopy, RNA-seq and the downstream bioinformatic analysism, as well as flow cytometry for the validation of a newly established cell model and its transcriptomic profile during an Talaromcyes marneffei infection.
Talaromyces marneffei is a thermally dimorphic fungus known to be the causative pathogen of talaromycosis - a potentially fatal fungal infection typically found in immunocompromised individuals. In accordance with the proposed transmission model, the airborne fungal conidia...

This file contains all raw and processed data of the thesis titled 'Nsp1 facilitates SARS-CoV-2 replication through calcium- calcineurin-NFATc2 signaling pathway'. The research study investigated how the SARS-CoV-2-encoded viral protein nsp1 hijacked the cellular calcieurin-NFAT pathway to substantiate SARS-CoV-2 replication and explored possible anti-nsp1 strategy to mitigate SARS-CoV-2 viral replication. This encrypted file contains the nanopore sequencing data for DelNSP1 BACmid and virus (Folder 1), the raw data for all infection assays, qPCR and luciferase...

This file contains results of histology, confocal microscopy, RNA-seq and the downstream bioinformatic analysism, as well as flow cytometry for the validation of a newly established cell model and its transcriptomic profile during an Talaromcyes marneffei infection.
Talaromyces marneffei is a thermally dimorphic fungus known to be the causative pathogen of talaromycosis - a potentially fatal fungal infection typically found in immunocompromised individuals. In accordance with the proposed transmission model, the airborne fungal conidia...

I study GATA2 and GATA3 role in trophoblast development and hematopoiesis development. I combine Crispr Cas9 for double knockout both GATA2 and GATA3. GATA2&3 DKO cell was characterized with IF stianing, WB. Then human TSC differentiation was performed. For hematopoiesis development, i conducte HEMO to test hematopoiesis differentiation efficiency of GATA2&3 DKO cells.

To select suitable human HCC cell lines for subsequent functional investigations, the mRNA expression and secretion levels of SPINK1 were assessed across various HCC cell lines. The analysis led to the selection of the SPINK1high Huh7 cell line for repression and the SPINK1low MHCC97L cell line for overexpression of SPINK1. Subsequently, the validation of SPINK1 expression was performed by performing western blot analysis on Huh7 cells with SPINK1 knockdown and MHCC97L cells treated with recombinant...

This is a poster presentation for AACR 2022 Annual Meeting

Our society is facing increasing burden of health care associated with cancer disease. At present, there are more than hundreds of anti-cancer drugs. At the same time, the number of compositions made from these clinically approved anti-cancer drugs is up to 1300-1500.[1] Among the clinically used anti-cancer drugs, cytotoxic platinum (II) compounds are the most extensively used in chemotherapy. In this project, the platinum(II) complexes bearing different bis-N-heterocyclic carbene and 1- (3-hydroxybenzo[b]thiophen-2-yl)ethanone (HO^O) ligand were...

This is a poster presentation for AACR 2022 Annual Meeting

The dataset includes all of the raw data involved in the PhD thesis. Each categorized data has been combined in a separate folder. A detailed description of the individual file has been involved in the README file.

To select suitable human HCC cell lines for subsequent functional investigations, the mRNA expression and secretion levels of SPINK1 were assessed across various HCC cell lines. The analysis led to the selection of the SPINK1high Huh7 cell line for repression and the SPINK1low MHCC97L cell line for overexpression of SPINK1. Subsequently, the validation of SPINK1 expression was performed by performing western blot analysis on Huh7 cells with SPINK1 knockdown and MHCC97L cells treated with recombinant...