1,377 Works

Roles of extracellular Ca2+ and pH on motility and flagellar waveform parameters in sturgeon spermatozoa

Sayyed Mohammad H. Alavi, Marek Rodina, Jacky Cosson, Martin Psenicka & Otomar Linhart
Ca2+ had no effect on symmetric flagellar behavior at pH 8.0. Wavelength and amplitude decreased signifi- cantly as a function of time post sperm activation, but number of curvature and waves increased. High concentrations of Ca2+ (2.5 mM) at high alkalinity (pH > 10.0) induced both acrosome reaction and asymmetric flagellar behavior.

Fish sperm cryopreservation in France: from laboratory studies to application in selective breeding programs

Pierrick Haffray, Catherine Labbé, & Gérard Maisse
Application of sperm cryopreservation by the fish farming industry is still limited, despite great interest in optimising genetic resource conservation; limiting risks associated with horizontal disease transmission; increasing genetic progress; and accelerating genetic selection from prime specimens, as in other livestock production. Several factors are listed. With the aim of developing coherent broodstock selection programmes, SYSAAF (Union of the French poultry and aquaculture broodstock industry), INRA and IMV-Technologies, standardised semen cryopreservation procedures between 1994 and...

Rainbow trout gonadotropin receptors are preferentially activated by their cognate ligand

Elisabeth Sambroni, Florence Le Gac, Bernard Breton & Jean-Jacques Lareyre
To better improve understanding of the molecular mechanisms involved in the actions of gonadotropins in fish, a structural and functional characterization of trout gonadotropin receptors has been undertaken. The present study describes two distinct gonadotropin receptors in trout showing similarities with those of other vertebrates but also differences in structural determinants of the FSHR. As observed in their mammalian counterparts, trout LH receptor (LHR) harbours a large N-terminal hormone binding domain with 9 leucine reach...

Introduction to Journées Techniques nationales (5th edition)

Cartographie des espèces de brochet récemment recensées en France : le brochet aquitain Esox aquitanicus et le brochet italien Esox cisalpinus

Gaël Denys, Théo Lauga & Agnès Dettai

Étude génétique des populations de truites en Auvergne-Rhône-Alpes

François Desmolles, Jean-Pierre Faure, Pierre Gres, Arnaud Caudron, Stéphane Nicolas, Mickaël Lelievre, Manuel Vallat & Stéphane Perrot

Apports et gestion de données télétransmises pour la gestion environnementale et halieutique de milieux aquatiques

Jean-Baptiste Fagot

Early sex reversal during embryonic development in the Nile tilapia

Carole Rougeot, Samane Y. Kanfitine, Christian Prignon, Vincent Gennotte & Charles Mélard
The aim of this work was to study the effect of hormonal sex-reversal treatment during embryonic development of Nile tilapia. XX and XY embryos were incubated in 17 α-methyltestosterone or in 17α-ethynylestradiol during embryonic development. Mean survival rates of embryos 5 days post-hatching ranged from 13.1% to 63.9%. XY embryos incubated in E2 displayed a significant proportion of females (from 50.6 to 79.0%) and XX embryos incubated in MT a significant proportion of males (from...

Cryopreservation of sperm of testicular neomales and stripped normal males of European perch (Perca fluviatilis L.)

Marek Rodina, Tomas Policar & Otomar Linhart
The present study demonstrated that stripped sperm of normal perch could be successfully frozen with hatching rate of fresh testicular sperm of neomales and stripped sperm of normal males were 42.5 and 49.3% at 12x105 spermatozoa per egg. In terms of neomale sperm, squeezing testes for collection of testicular sperm is not recommended for cryopreservation due to low sperm velocity, motility and hatching rate. Both sperm velocity and motility showed a significant decrease in the...

High transcript level of a fatty acid-binding protein 11 but not of vitellogenin receptor is correlated to ovarian follicle atresia in a teleost fish (Solea senegalensis)

Maria J. Agulleiro, Michèle André, Sofia Morais, Joan Cerdá & Patrick J. Babin
The expression level of vtgr transcripts may be used as a precocious functional marker to quantify the number of oocytes recruited for vitellogenesis and fabp11 mRN A may be a very useful molecular marker for determining cellular events and environmental factors that regulate follicular atresia in fish.

Determinism of the quality of reproduction in male Eurasian perch, Perca fluviatilis: A multifactorial study

Neil Wang, Marek Rodina, Jean-Noël Gardeur, Jean-Thomas Vuillard, Tomas Policar, Emilie Henrotte, Syaghalirwa Mandiki, Patrick Kestemont, Otomar Linhart & Pascal Fontaine
The multifactorial determinism of the quality of male perch reproduction was investigated. A schematic model of regulation is proposed.

Motility characteristics of spermatozoa in cod (Gadus morhua) and hake (Merluccius merluccius)

Jacky Cosson, Anne-Laure Groison, Marc Suquet & Christian Fauvel
As in many marine fish, motility is triggered by osmotic pressure (OP) in cod and hake spermatozoa and motility ranges several minutes with a high velocity (above 50 μm/s) period restricted to 100 s. A decrease in the flagellar beat frequency (BF) is partly responsible of this briefness. Detailed observations of the flagella during the motility period showed: 1) sea water provokes OP damages (blebs) which impair the correct wave propagation 2) waves become restricted...

Deduced primary structures of three vitellogenins and specific binding to putative multiple ovarian receptors in white perch (Morone americana)

Benjamin J. Reading, Naoshi Hiramatsu, Takahiro Matsubara, Akihiko Hara & Craig V. Sullivan
Three complete cDNA s encoding different forms of vitellogenin (vtg) were cloned from a white perch (Morone americana) liver cDNA library. The two longest cDNA s encoded Vtgs with a complete suite of yolk protein domains that, based on comparisons with vtg sequences from other species, were categorized as VtgAa and VtgAb. The shorter cDNA encoded a Vtg that lacked a phosvitin domain, had a shortened C-terminus, and was categorized as VtgC. Ligand blots and...

Seasonal-related homologous regulation of goldfish liver Estrogen receptor subtypes

Erik R. Nelson & Hamid R. Habibi
Treatment with estrogen was found to increase expression of estrogen receptor subtypes (ERα, ERβ1 or ERβ2) in the liver of goldfish during regressed gonadal stage. Here, we test the hypothesis that homologous ER regulation may occur in a seasonal-dependant manner. To make seasonal comparison, we investigated homologous regulation of the ERs expression in the livers of goldfish at mid-late stage of gonadal recrudescence. The results demonstrate that homologous regulation of the ER subtypes are different...

French ichthyological records for 2018

Samuel P. Iglésias &
Uncommon records of fishes may evidence local or global changes in fish composition resulting from environmental change or anthropogenic activities. Significant records of uncommon marine fishes, including migrant, non-native, cryptic, rare and threatened species, collected in French waters or by French vessels in European waters, observed by scuba divers or beachgoers, are reported for the year 2018. They include first, new, rare and unusual records for the following 52 species: Hexanchus griseus, Squatina squatina, Gymnura...

Effects of gonadotropin-releasing hormone agonist (GnRHa) administration on male germ cell apoptosis in captive Atlantic bluefin tuna (Thunnus thynnus L.)

Aldo Corriero, Antonio Medina, Constantinos C. Mylonas, Nicoletta Santamaria, Michele Losurdo, Francisco J. Abascal, Daniela Spedicato, Aida Aprea, Christopher R. Bridges & Gregorio De Metrio
The effects of gonadotropin-releasing hormone agonist (GnRHa) administration on testicular maturation and apoptosis of male germ cells of Atlantic bluefin tuna (Thunnus thynnus L.) reared in captivity were evaluated. Individuals classified at early and late spermatogenesis were equally present among untreated and GnRHa-treated fish. A lower rate of germ cell apoptosis was observed in GnRHa-treated individuals than in untreated controls. The present study indicates that: (i) wild-caught male Atlantic bluefin tuna reared for 1 to...

Estrogenic response of wild roach from the Seine River (France)

Perrine Maltret-Geraudie, Marie Gerbron & Christophe Minier
The aquatic environment is the final destination for most chemicals released in the environment, as a consequence freshwater populations are inevitably exposed and may suffer from adverse effects. Fish have been widely used as models for determining the effect of environmental pollutants on vertebrate reproductive function. Several studies have demonstrated endocrine disrupting effects on fish living in rivers that receive treated sewage effluents. To investigate the estrogenic effects of effluents from the Seine River, plasmatic...

Specific binding of exogenous sex hormones and chemical substances to sex hormone-binding globulin (SHBG) in carp and rainbow trout

Masaki Nagae, Yuichi Suminami, Hiroko Kitagawa, Ken Ichirou Shiroyama, Nobuyuki Ohkubo & Takahiro Matsubara
The binding characteristics of sex hormone-binding globulin (SHBG ), the most important transporter of sex steroids in blood, was investigated using various kinds of sex steroids in carp and rainbow trout in vivo and in vitro. In vitro binding assay using various kinds of sex steroids revealed androgens (T and 11-KT), estrogens (E2 and EE2) and progestins (17α-P and 17α,20b-DP) have a high affinity to SHBG. However, the affinity of estrone, cortisol, and bisphenol-A to...

Fish egg quality and oocyte transcriptome. A new approach using cDNAs microarrays on oocytes after two ovulation perturbations

Emilie Bonnet, Bernard Jalabert, Alexis Fostier & Julien Bobe
The purpose of this study was to investigate the effect of photoperiod-advanced spawning and hormonallyinduced ovulation on egg quality and egg transcriptome. Experimentations were performed using 3 groups of rainbow trout (Oncorhynchus mykiss) from an autumn-spawning strain: (i) natural spawning, (ii) photoperiod-advanced, and (iii) hormonally- induced group. For each female, egg quality was evaluated by measuring survival and malformations at yolk-sac resorption. Transcriptome analysis was performed using AGENAE microarrays displaying 9,152 rainbow trout cDNA s....

Review of Giuris (Teleostei: Eleotridae) from Indo-Pacific islands, with description of three new species

Philippe Keith & Marion I. Mennesson
Giuris species from the Indo-Pacific area are reviewed. Eight species are recognized including three new species. These are described using genetic and morphomeristic approaches. The species differ by a high percentage of divergence in partial COI gene (495 bp) and by several characters including the number of pectoral fin rays, the number of scales in transverse forward series, the number of scales around the eye, the interorbital length, and the body depth at first dorsal...

Reevaluation of the Perciformes from the Late Cretaceous Marília Formation of Brazil and its confirmation as a Siluriformes

Paulo M. Brito, François J. Meunier & Yuri M. Alves
A fossil spine, originally described as belonging to Perciformes from the Late Cretaceous Marília Formation, Bauru Basin, Southeastern Brazil, is here restudied based on paleohistology. In addition to the overall morphology of the spine, our histological results point to the presence of pseudolamellar bone with embedded star-shaped osteocytes. These results confirm the recent redescription of this specimen as a Siluriformes, and confirm the absence, so far, of Percomorpha in freshwater deposits from the Cretaceous of...

Gonadal development and spawning rhythm in mudskipper (Periophthalmus modestus)

Kiyoshi Soyano, Tatsuya Higuchi, Minoru Kobayashi, Megumi Takushima, Jun-Ya Aoki & Masaki Nagae
Reproductive and spawning cycle of mudskipper were examined. The reproductive season in this species was between May and early August. The final oocyte maturation and spawning in natural conditions depend on the endocrine changes that are induced by environmental cue such as tidal variability.

First record of Tortonese’s goby, Pomatoschistus tortonesei, from Tunisia

Randa Mejri, Domia Menif, Ibtissem Louiz & Oum Kalthoum Ben Hassine
The gobiid Pomatoschistus tortonesei Miller, 1968, which geographic distribution was restricted to Marsala lagoon (Western Sicily, Italy) and Farawa lagoon (Libya), was found for the first time in six Tunisian lagoons. The meristic counts and morphometric measurements of P. tortonesei are determined on the basis of 27 specimens.

Ecology and diet of the endemic Telestes ukliva (Cyprinidae) in the Cetina River system, Croatia

Davor Zanella, Zlatko Mihaljević, Milorad Mrakovčić & Marko Ćaleta
The first data on the population ecology and diet of the endemic ukliva dace (Telestes ukliva Heckel, 1843) are presented. Along the Cetina River system, the ukliva dace accounted for 38.4% of the overall fish fauna abundance, but only 6.4% of total fish biomass. The ukliva dace population exhibited migratory behaviour during the year and showed a strong temporal component; inhabiting the river main course in spring and summer, and colonising springs and subterranean waters...

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