1,196 Works

Early sex reversal during embryonic development in the Nile tilapia

Carole Rougeot, Samane Y. Kanfitine, Christian Prignon, Vincent Gennotte & Charles Mélard
The aim of this work was to study the effect of hormonal sex-reversal treatment during embryonic development of Nile tilapia. XX and XY embryos were incubated in 17 α-methyltestosterone or in 17α-ethynylestradiol during embryonic development. Mean survival rates of embryos 5 days post-hatching ranged from 13.1% to 63.9%. XY embryos incubated in E2 displayed a significant proportion of females (from 50.6 to 79.0%) and XX embryos incubated in MT a significant proportion of males (from...

Motility characteristics of spermatozoa in cod (Gadus morhua) and hake (Merluccius merluccius)

Jacky Cosson, Anne-Laure Groison, Marc Suquet & Christian Fauvel
As in many marine fish, motility is triggered by osmotic pressure (OP) in cod and hake spermatozoa and motility ranges several minutes with a high velocity (above 50 μm/s) period restricted to 100 s. A decrease in the flagellar beat frequency (BF) is partly responsible of this briefness. Detailed observations of the flagella during the motility period showed: 1) sea water provokes OP damages (blebs) which impair the correct wave propagation 2) waves become restricted...

Review of Giuris (Teleostei: Eleotridae) from Indo-Pacific islands, with description of three new species

Philippe Keith & Marion I. Mennesson
Giuris species from the Indo-Pacific area are reviewed. Eight species are recognized including three new species. These are described using genetic and morphomeristic approaches. The species differ by a high percentage of divergence in partial COI gene (495 bp) and by several characters including the number of pectoral fin rays, the number of scales in transverse forward series, the number of scales around the eye, the interorbital length, and the body depth at first dorsal...

Reevaluation of the Perciformes from the Late Cretaceous Marília Formation of Brazil and its confirmation as a Siluriformes

Paulo M. Brito, François J. Meunier & Yuri M. Alves
A fossil spine, originally described as belonging to Perciformes from the Late Cretaceous Marília Formation, Bauru Basin, Southeastern Brazil, is here restudied based on paleohistology. In addition to the overall morphology of the spine, our histological results point to the presence of pseudolamellar bone with embedded star-shaped osteocytes. These results confirm the recent redescription of this specimen as a Siluriformes, and confirm the absence, so far, of Percomorpha in freshwater deposits from the Cretaceous of...

Seasonal-related homologous regulation of goldfish liver Estrogen receptor subtypes

Erik R. Nelson & Hamid R. Habibi
Treatment with estrogen was found to increase expression of estrogen receptor subtypes (ERα, ERβ1 or ERβ2) in the liver of goldfish during regressed gonadal stage. Here, we test the hypothesis that homologous ER regulation may occur in a seasonal-dependant manner. To make seasonal comparison, we investigated homologous regulation of the ERs expression in the livers of goldfish at mid-late stage of gonadal recrudescence. The results demonstrate that homologous regulation of the ER subtypes are different...

Estrogenic response of wild roach from the Seine River (France)

Perrine Maltret-Geraudie, Marie Gerbron & Christophe Minier
The aquatic environment is the final destination for most chemicals released in the environment, as a consequence freshwater populations are inevitably exposed and may suffer from adverse effects. Fish have been widely used as models for determining the effect of environmental pollutants on vertebrate reproductive function. Several studies have demonstrated endocrine disrupting effects on fish living in rivers that receive treated sewage effluents. To investigate the estrogenic effects of effluents from the Seine River, plasmatic...

French ichthyological records for 2018

Samuel P. Iglésias &
Uncommon records of fishes may evidence local or global changes in fish composition resulting from environmental change or anthropogenic activities. Significant records of uncommon marine fishes, including migrant, non-native, cryptic, rare and threatened species, collected in French waters or by French vessels in European waters, observed by scuba divers or beachgoers, are reported for the year 2018. They include first, new, rare and unusual records for the following 52 species: Hexanchus griseus, Squatina squatina, Gymnura...

Effects of gonadotropin-releasing hormone agonist (GnRHa) administration on male germ cell apoptosis in captive Atlantic bluefin tuna (Thunnus thynnus L.)

Aldo Corriero, Antonio Medina, Constantinos C. Mylonas, Nicoletta Santamaria, Michele Losurdo, Francisco J. Abascal, Daniela Spedicato, Aida Aprea, Christopher R. Bridges & Gregorio De Metrio
The effects of gonadotropin-releasing hormone agonist (GnRHa) administration on testicular maturation and apoptosis of male germ cells of Atlantic bluefin tuna (Thunnus thynnus L.) reared in captivity were evaluated. Individuals classified at early and late spermatogenesis were equally present among untreated and GnRHa-treated fish. A lower rate of germ cell apoptosis was observed in GnRHa-treated individuals than in untreated controls. The present study indicates that: (i) wild-caught male Atlantic bluefin tuna reared for 1 to...

Egg quality in domesticated and wild seabass (Dicentrarchus labrax): A proteomic analysis

Amélie Crespel, Hélène Rime, Erwann Fraboulet, Julien Bobe & Christian Fauvel
In order to improve our understanding of factors determining egg quality in fish, seabass egg batches obtained from 34 females held under standard conditions, were assessed for biological performances and a fraction from each batch was subjected to proteomic analysis. Comparison of proteomic patterns from eggs of different quality revealed differential protein spots among which one was identified as Nucleoside Diphosphate Kinase, a highly conserved protein involved in cell proliferation. This study shows that molecular...

Effects of hormone injections on tolerance to cryopreservation of ayu spermatozoa

Ken-Ichi Yokoi, Koji Yamane, Madoka Tsutaka, Shinichi Yamamoto, Mitsuki Gouda, Kenji Takii & Hiromi Ohta
We examined the effects of maturation stimulating hormone, salmon pituitary extract (SPE) or 17α-hydroxyprogesterone (17α-OHP), injections on the post-thaw motility of ayu spermatozoa. Comparative post-thaw motility (percent motility of post-thawed spermatozoa/that of fresh spermatozoa x100) in the Ringer control group showed higher percent motility in the late time than in the early time of the spawning season. Post-thaw motility was higher in the SPE and 17α-OHP groups than in the Ringer control group. These results...

Specific binding of exogenous sex hormones and chemical substances to sex hormone-binding globulin (SHBG) in carp and rainbow trout

Masaki Nagae, Yuichi Suminami, Hiroko Kitagawa, Ken Ichirou Shiroyama, Nobuyuki Ohkubo & Takahiro Matsubara
The binding characteristics of sex hormone-binding globulin (SHBG ), the most important transporter of sex steroids in blood, was investigated using various kinds of sex steroids in carp and rainbow trout in vivo and in vitro. In vitro binding assay using various kinds of sex steroids revealed androgens (T and 11-KT), estrogens (E2 and EE2) and progestins (17α-P and 17α,20b-DP) have a high affinity to SHBG. However, the affinity of estrone, cortisol, and bisphenol-A to...

Improved storage conditions for unfertilized rainbow trout (Oncorhynchus mykiss) eggs

Marina Komrakova & Wolfgang Holtz
Unfertilized rainbow trout eggs were kept at 2 ± 1°C under O2, CO2, N2, air or expired air. Eggs were stored either loose or sealed in PE foil. After 5, 10, 15 and 20 d the pH was recorded and the eggs were fertilized with cryopreserved semen and incubated to the eyed egg stage. Under CO2 eggs succumbed within 1 d. Between air and exhaled air there was no difference. With loose eggs air was...

Sperm chromatin fragmentation and oxidization during short- and long-term storage of sex reversed rainbow trout (Oncorhynchus mykiss) males

Serafín Pérez-Cerezales, Sonia Martínez-Páramo & M. Paz Herráez
Sperm storage is a basic tool for reproductive biotechnology that causes cell damage at different levels. Our objective was to detect DNA strand breaks or nucleotide oxidization promoted by short and long-term storage in spermatozoa from sex-reversed rainbow trout, using the Comet assay and previous digestion with specific endonucleases. Short-term storage promotes strand breaks and oxidization, especially in guanines. Cryopreservation increases DNA fragmentation, but apparently does not cause oxidization, suggesting possible apoptotic events.

High transcript level of a fatty acid-binding protein 11 but not of vitellogenin receptor is correlated to ovarian follicle atresia in a teleost fish (Solea senegalensis)

Maria J. Agulleiro, Michèle André, Sofia Morais, Joan Cerdá & Patrick J. Babin
The expression level of vtgr transcripts may be used as a precocious functional marker to quantify the number of oocytes recruited for vitellogenesis and fabp11 mRN A may be a very useful molecular marker for determining cellular events and environmental factors that regulate follicular atresia in fish.

Gonadal development and spawning rhythm in mudskipper (Periophthalmus modestus)

Kiyoshi Soyano, Tatsuya Higuchi, Minoru Kobayashi, Megumi Takushima, Jun-Ya Aoki & Masaki Nagae
Reproductive and spawning cycle of mudskipper were examined. The reproductive season in this species was between May and early August. The final oocyte maturation and spawning in natural conditions depend on the endocrine changes that are induced by environmental cue such as tidal variability.

Fish egg quality and oocyte transcriptome. A new approach using cDNAs microarrays on oocytes after two ovulation perturbations

Emilie Bonnet, Bernard Jalabert, Alexis Fostier & Julien Bobe
The purpose of this study was to investigate the effect of photoperiod-advanced spawning and hormonallyinduced ovulation on egg quality and egg transcriptome. Experimentations were performed using 3 groups of rainbow trout (Oncorhynchus mykiss) from an autumn-spawning strain: (i) natural spawning, (ii) photoperiod-advanced, and (iii) hormonally- induced group. For each female, egg quality was evaluated by measuring survival and malformations at yolk-sac resorption. Transcriptome analysis was performed using AGENAE microarrays displaying 9,152 rainbow trout cDNA s....

Cryopreservation of sperm of testicular neomales and stripped normal males of European perch (Perca fluviatilis L.)

Marek Rodina, Tomas Policar & Otomar Linhart
The present study demonstrated that stripped sperm of normal perch could be successfully frozen with hatching rate of fresh testicular sperm of neomales and stripped sperm of normal males were 42.5 and 49.3% at 12x105 spermatozoa per egg. In terms of neomale sperm, squeezing testes for collection of testicular sperm is not recommended for cryopreservation due to low sperm velocity, motility and hatching rate. Both sperm velocity and motility showed a significant decrease in the...

Powder coconut water (ACP®) as extender for semen cryopreservation of Brazilian migratory fish species

Ana T.M. Viveiros, Alexandre N. Maria, Laura H. Orfão, Mª Audalia M. Carvalho & José F. Nunes
Methods to dehydrate coconut water and produce a stable and standardized powder coconut water (ACP®) has been developed. The use of ACP® as semen extender has been tested in many mammalian species, but this is the first report on fish species. The aim of this study was to compare ACP® with our standard freezing medium for semen cryopreservation of three Brazilian migratory fish species. ACP® was tested in combination with methylglycol (MG) or dimethyl sulphoxide...

Left-Right gene expression asymmetry in gonads of rainbow trout, Oncorhynchus mykiss, following masculinization treatments with androgens

Mathieu Guillevic & Yann Guiguen
Following masculinization of an all female genetic population of rainbow trout larvae by repetitive immersions with 17α-methyltestosterone (17 αMT) we investigate if we could characterize a L/R gene expression asymmetry in the gonads of these treated fish. The 17 αMT immersion treatment resulted in an overall masculinization percentage of 29%. Gene expression of 18 genes reveals that most of the genes investigated exhibit clear left/right (L/R) differences in their gonadal expression profiles. This L/R asymmetry...

‘Post-sex differentiational’ sex reversal in the female common carp (Cyprinus carpio)

Satoshi Ogawa, Minoru Akiyoshi, Masahito Higuchi, Masaru Nakamura & Toshiaki Hirai
Gonadal plasticity after sex differentiation has been verified in the female common carp. One-year-old genetically controlled female (XX) carps were fed with Fadrozole, an aromatase inhibitor (AI), and the gonads were subjected to histological analysis. Testicular lobule-like structures and cysts of spermatocytes began to appear after 8 weeks of treatment, and spermatozoa were initially observed at week 16 and then spread to the whole gonad in some individuals by week 18. These observations show that...

Melatonin receptors in the brain and the pituitary of a Venezuelan catfish, “Sierra Negra” (Oxydoras sifontesi): A preliminary 2-[125I] iodomelatonin binding study

Hilda Y. Guerrero, Elizabeth Cardillo, Gilmer Vanegas, Germán Poleo & Daissy Marcano
We have studied melatonin binding sites in the brain of the tropical catfish O. sifontesi using in vitro autoradiography and receptor binding assay. Specific binding was found in brain areas involved in the processing of visual information and in the pituitary. Binding was saturable around 150 pM. KD and Bmax were calculated.

Quelques aspects reproductifs de l’anchois (Engraulis encrasicolus) de la pêche artisanale du littoral est ivoirien

Siaka Ouattara, Agathe Fantodji & Mamadou Ouattara
Quelques aspects du cycle de reproduction de l’anchois (Engraulis encrasicolus) de la pêche artisanale ont été étudiés de septembre 2004 à mars 2006. Les échantillonnages ont été réalisés dans des campements et villages, le long du littoral ivoirien où les sennes de plage et les sennes tournantes coulissantes constituent les engins de la pêche artisanale utilisés pour la capture de ces poissons entre 6 à 10 m de profondeur. La sex-ratio globale observée est de...

Sexual changes in the neurosteroids of Indian major carp, Labeo rohita (Ham.)

Moses Inbaraj & Winkins Santosh
The synthesis of steroids in different regions of the fish brain needs to be studied in order to understand the control mechanism of ‘neurosteroids’ on reproduction in both males and females. In the present study, the synthesis of sex steroids testosterone (T) and estradiol-17b (E2) was studied in different regions of the brain in the carp L. rohita, by using 17α-OHprogesterone (17α-OHP) as a precursor in in vitro incubations. A sex dependent difference was observed...

New data on the structure and the chondrocyte populations of the haemal cartilage of abdominal vertebrae in the adult carp Cyprinus carpio (Ostariophysii, Cyprinidae)

Louise Zylberberg & François J. Meunier
The bulk of cartilage retained in the haemal arch of the abdominal vertebrae of adult carps was examined using scanning and transmission electron microscopy. A special attention was devoted to the mineralization of the cartilage. The mineralization is spheritic but the processes differ in the proximal and distal parts. The proximal hyaline cartilage contacts the lamellar bone of the centrum without an intermediate chondroid bone; it mineralizes although chondrocytes bearing a hypertrophic phenotype were not...

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