26 Works

Host Factor Experiment (SCL006-P)

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Purpose: To obtain samples for transcriptional analysis and proteomics in triplicate using wild typeicSARS urbani and icSARS Bat SRBD mutant in 2B4 cells/sorted Calu3 cells with high ACE2 expression. Overview of Experiment: Time Points = 0, 7, 12, 24, 30, 36, 48, 54, 60 and 72 hrs post infection. (Note: there is no time point at 7 h for icSARS Bat SRBD.) Done in triplicate for both RNA and Protein. Triplicates are defined as 3...

Host Factor Experiment (Tseng01_Infected_2B4_Expression)

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Human lung epithelial cells are likely among the first targets to encounter invading severe acute respiratory syndrome-associated coronavirus (SARS-CoV). Not only can these cells support the growth of SARS-CoV infection, but they are also capable of secreting inflammatory cytokines to initiate and, eventually, aggravate host innate inflammatory responses, causing detrimental immune-mediated pathology within the lungs. Thus, a comprehensive evaluation of the complex epithelial signaling to SARS-CoV is crucial for paving the way to better understand...

Host Factor Experiment (SM007-R)

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Purpose: To obtain lung samples from Tnfrsf1a/1b knock out mutant mice infected with SARS MA15 virus for transcriptional analyses. Details: Time Points = days 2, 4 and 7 post-infection; 2-4 replicate mice for each condition; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^4 pfu.

Host Factor Experiment (ECL001-R)

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Purpose: To obtain samples from 2B-4 cells (a clonal derivative of Calu-3 cells) infected with MERS-CoV (previously called Hu CoV EMC virus) for transcriptional analysis. MERS-CoV and mock infected, the time points are 0, 3, 7, 12, 18 and 24h post-infection, except for mocks where the 18h was excluded. There are triplicate samples for all conditions where triplicates are defined as 3 different wells, plated at the same time using the same cell stock for...

Host Factor Experiment (EjuanesL_AUM_02_Mouse_Lung_Expression)

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A recombinant severe acute respiratory syndrome coronavirus (SARS-CoV) lacking the envelope (E) protein is attenuated in vivo. Here we report that E protein PDZ-binding motif (PBM), a domain involved in protein-protein interactions, is a major determinant of virulence. Elimination of SARS-CoV E protein PBM by using reverse genetics caused a reduction in the deleterious exacerbation of the immune response triggered during infection with the parental virus and virus attenuation. Cellular protein syntenin was identified to...

Host Factor Experiment (SM004-R)

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Purpose: To obtain lung samples from C57BL6, TIMP1 or Serpine1 (PAI1) knock-out mice infected with SARS MA15 virus for transcriptional analysis. Details: Time Points = 4 and 7 days post-infection; 2-4 replicates for infected and mock mice; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^4 pfu.

Host Factor Experiment (TCL001-R)

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Purpose: To obtain samples from Calu-3 cells exposed to either interferon (IFN) _ or _ for transcriptional analysis. For IFN_ and corresponding mock exposed-cells the time points are 0, 3, 6, and 18h post-exposure. For IFN_ and corresponding mock exposed-cells the time points are 0, 3, 6, and 21h post-exposure. There are triplicate samples for all conditions, where triplicates are defined as 3 different wells, plated at the same time using the same cell stock...

Host Factor Experiment (SM001-R)

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Purpose: To look at the host response to different doses across 4 time points after infection. Samples were collected for both transcriptomics and proteomics.General Design: 20 week-old C57Bl6 mice; ThreeDoses = 1E2, 1E3,1E4 and 1E5 (PFU); Time points of 1, 2, 4 and 7 days; ~5 mice/time point for infections; 3 mice/timepoint for time matched mocks

Host Factor Experiment (SM015-R)

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Purpose: To obtain lung samples from Tnfrsf1a/1b knock out mutant mice infected with SARS MA15 virus for transcriptional analyses. Details: Time Points = days 4 and 7 post-infection; 2-3 replicate mice for each condition; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^5 pfu.

Host Factor Experiment (EjuanesL_AUM_01_VE6_MA104_Expression)

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Severe acute respiratory syndrome virus (SARS-CoV) that lacks the envelope (E) gene (rSARS-CoV-_E) is attenuated in vivo. To identify factors that contribute to rSARS-CoV-_E attenuation, gene expression in cells infected by SARS-CoV with or without E gene was compared. Twenty-five stress response genes were preferentially upregulated during infection in the absence of the E gene. In addition, genes involved in signal transduction, transcription, cell metabolism, immunoregulation, inflammation, apoptosis and cell cycle and differentiation were differentially...

Host Factor Experiment (SCL008-R)

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Purpose: To obtain samples for transcriptional and proteomic analysis using wild type icSARS CoV and icSARS ExoNI and icSARS dNSP16 mutants in 2B-4 cells/sorted Calu-3 cells with high ACE2 expression. Details: Time Points = 0, 7, 12, 24, 36, 48, 60 and 72h post infection (Note: only 0, 24, 48 and 72hr samples analysed for proteomics); Done in triplicate for RNA and quadruplicate for protein; Replicates are defined as 3 or 4 different wells, plated...

Host Factor Experiment (SM009-R)

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Purpose: To obtain lung samples from C57BL6 or PLAT knock-out mice infected with SARS MA15 virus for transcriptional analysis. Details: Time Points = 4 and 7 days post-infection; 2-3 replicates for infected and mock mice; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^5 pfu.

Host Factor Experiment (SM003-R)

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Purpose: To obtain lung samples from C57BL6 mice infected with , icSARS, SARS MA15 or SARS BatSRBD mutant viruses for transcriptional analysis. Details: Time Points = 1, 2, 4 and 7 days post-infection; 3-5 replicates for infected and mock mice; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^5 pfu for icSARS, 10^4 or 10^5pfu for MA15 and 10^4pfu BatSRBD.

Host Factor Experiment (SM020-R)

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Purpose: To obtain lung samples from ppp1r14c knockout mutant mice infected with SARS MA15 virus for transcriptional analyses. Details: Time Points = days 4 and 7 post-infection; 2-3 replicate mice for each condition; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^5 pfu.

Host Factor Experiment (SHAE004-R)

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Purpose: To obtain samples from primary Human Airway Epitheilal (HAE) cells infected with SARS-CoV, SARS deltaORF6, SARS BatSRBD mutants or A/CA/04/2009 (H1N1) influenza for transcriptional analysis. The time points are 0, 12, 24, 36, 48, 60, 72, 84 and 96h post-infection for SARS viruses (except SHAE003 which did not include the 12 and 36h timepoints), 0, 6, 12, 18, 24, 36 and 48h post-infection for H1N1 and 0, 6, 12, 18, 24, 36, 48, 60,...

Host Factor Experiment (SM014-R)

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Purpose: To obtain lung samples from C57BL6 mice infected with SARS MA15 and nsp16 mutant viruses for transcriptional analysis. Details: Time Points = 1, 2, 4 and 7 days post infection; 3-4 replicates for infected and 3 replicates for mock mice; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^5 pfu.

Host Factor Experiment (SHAE003-R)

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Purpose: To obtain samples from primary Human Airway Epitheilal (HAE) cells infected with SARS-CoV, SARS deltaORF6, SARS BatSRBD mutants or A/CA/04/2009 (H1N1) influenza for transcriptional analysis. The time points are 0, 12, 24, 36, 48, 60, 72, 84 and 96h post-infection for SARS viruses (except SHAE003 which did not include the 12 and 36h timepoints), 0, 6, 12, 18, 24, 36 and 48h post-infection for H1N1 and 0, 6, 12, 18, 24, 36, 48, 60,...

Host Factor Experiment (SHAE002-R)

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Purpose: To obtain samples from primary Human Airway Epitheilal (HAE) cells infected with SARS-CoV, SARS deltaORF6, SARS BatSRBD mutants or A/CA/04/2009 (H1N1) influenza for transcriptional analysis. The time points are 0, 12, 24, 36, 48, 60, 72, 84 and 96h post-infection for SARS viruses (except SHAE003 which did not include the 12 and 36h timepoints), 0, 6, 12, 18, 24, 36 and 48h post-infection for H1N1 and 0, 6, 12, 18, 24, 36, 48, 60,...

Host Factor Experiment (SCL006-R)

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Purpose: To obtain samples for transcriptional analysis and proteomics in triplicate using wild typeicSARS urbani and icSARS Bat SRBD mutant in 2B4 cells/sorted Calu3 cells with high ACE2 expression. Overview of Experiment: Time Points = 0, 7, 12, 24, 30, 36, 48, 54, 60 and 72 hrs post infection. (Note: there is no time point at 7 h for icSARS Bat SRBD.) Done in triplicate for both RNA and Protein. Triplicates are defined as 3...

Host Factor Experiment (SCL005-P)

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Purpose: To obtain samples for transcriptional and proteomic analysis using wild type and icSARS delta ORF6 virus in 2B-4 cells/sorted Calu-3 cells with high ACE2 expression. Details: Time Points = 0, 3, 7, 12, 24, 30, 36, 48, 54, 60 and 72h post infection; Done in triplicate for both RNA and protein; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks...

Host Factor Experiment (SM001-P)

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Purpose: To look at the host response to different doses across 4 time points after infection. Samples were collected for both transcriptomics and proteomics.General Design: 20 week-old C57Bl6 mice; ThreeDoses = 1E2, 1E3,1E4 and 1E5 (PFU); Time points of 1, 2, 4 and 7 days; ~5 mice/time point for infections; 3 mice/timepoint for time matched mocks

Host Factor Experiment (SM019-R)

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Purpose: To obtain lung samples from Tnfrsf1b knock out mutant mice infected with SARS MA15 virus for transcriptional analyses. Details: Time Points = days 4 and 7 post-infection; 2-3 replicate mice for each condition; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^5 pfu.

Host Factor Experiment (DiegoM_AUM_01_Mouse_SARS_Expression)

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Severe acute respiratory syndrome coronavirus (SARS-CoV) causes a respiratory disease with a mortality rate of 10%. A mouse-adapted SARS-CoV (SARS-CoV-MA15) lacking the envelope (E) protein (rSARS-CoV-MA15-_E) is attenuated in vivo. To identify E protein regions and host responses that contribute to rSARS-CoV-MA15-_E attenuation, several mutants (rSARS-CoV-MA15-E*) containing point mutations or deletions in the amino-terminal or the carboxy-terminal regions of the E protein were generated. Amino acid substitutions in the amino terminus, or deletion of regions...

Host Factor Experiment (SM012-R)

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Purpose: To obtain lung samples from C57BL6 mice infected with SARS MA15 and deltaORF6 mutant viruses for transcriptional analysis. Details: Time Points = 1, 2, 4 and 7 days post infection; 2-3 replicates for infected and mock mice; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^5 pfu.

Host Factor Experiment (SCL008-P)

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Purpose: To obtain samples for transcriptional and proteomic analysis using wild type icSARS CoV and icSARS ExoNI and icSARS dNSP16 mutants in 2B-4 cells/sorted Calu-3 cells with high ACE2 expression. Details: Time Points = 0, 7, 12, 24, 36, 48, 60 and 72h post infection (Note: only 0, 24, 48 and 72hr samples analysed for proteomics); Done in triplicate for RNA and quadruplicate for protein; Replicates are defined as 3 or 4 different wells, plated...

Registration Year

  • 2020
    26

Resource Types

  • Dataset
    26

Affiliations

  • University of Washington
    21