47 Works

Host Factor Experiment (ICL012-R)

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Purpose: To obtain samples from Calu-3 cells infected with A/Vietnam/1203-CIP048_RG4/2004 (H5N1). Details: Time Points = 0, 3, 7, 12, 18 and 24hrs post infection were taken for mutant virus while wild type virus was only tested at 7 and 24hrs post-infection; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of samples;...

Host Factor Experiment (IM007-P)

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Purpose: To obtain lung samples from C57BL6 mice infected with Vietnam/1203-CIP048_RG4/2004 (H5N1) for both transcriptional and proteomic analyses. Details: Time Points = 1, 2, 4 and 7 days post infection; 5 replicates for infected mice and triplicate mice for the mocks; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^3 and 10^4 PFU.

Host Factor Experiment (ICL004-R)

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Purpose: To obtain samples from Calu-3 cells infected with A/Vietnam/1203/2004(H5N1) for both transcriptional and proteomics analyses. Details: Time Points = 0, 3, 7, 12, 18, and 24 h post infection; Done in triplicate for both RNA and Protein; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of samples; Culture medium (the...

Host Factor Experiment (E_R_MDM_H1N1)

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Human monocyte-derived macrophages (MDM) serve as a model for resident alveolar macrophages (AM) in the human respiratory tract. MDM cells were analyzed at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.

Host Factor Experiment (E_R_C57BL/6_FLU)

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Analysis of C57BL/6 mouse lungs infected with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53. A/Wyoming/03/03 (H3N2) influenza virus replicates poorly in mice and lung tissue collected from mice inoculated with this virus did not carry viral loads detectable by plaque assay.

Host Factor Experiment (ICL010-P)

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Purpose: To obtain samples from Calu-3 cells infected with A/Netherlands/602/2009 (H1N1) and A/CA/04/09 (H1N1) for transcriptional analysis and proteomics analysis for A/Netherlands/602/2009. For A/Netherlands/602/2009 and mock infected the time points are 0, 3, 7, 12, 18, 24, 30, 36 and 48 h post infection. For A/CA/04/09 the time points are 0,12, 24 and 48 h post infection.There are triplicate samples for all conditions for both transcriptional and proteomics analyses. Triplicates are defined as 3 different...

Host Factor Experiment (ICL011-P)

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Purpose: To obtain samples from Calu-3 cells infected with mutants A/Vietnam/1203-CIP048_RG2/2004 (H5N1) and A/Vietnam/1203-CIP048_RG3/2004 (H5N1). Details: Time Points = 0, 3, 7, 12, 18 and 24hrs post infection were taken for mutant viruses while wild type virus was only tested at 7 and 24hrs post-infection; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock...

Host Factor Experiment (ICL012-P)

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Purpose: To obtain samples from Calu-3 cells infected with A/Vietnam/1203-CIP048_RG4/2004 (H5N1). Details: Time Points = 0, 3, 7, 12, 18 and 24hrs post infection were taken for mutant virus while wild type virus was only tested at 7 and 24hrs post-infection; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of samples;...

Host Factor Experiment (IM010-R)

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Purpose: To obtain lung samples from C57BL6 mice infected with Vietnam/1203/2004 (H5N1) for transcriptional analyses. Details: Mouse strains = WT, IDO1 knock-out and Tnfrsf1b knock-out; Time Points = 2 and 6 days post-infection; 2-3 replicates for each mouse strain; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 1000 pfu.

Host Factor Experiment (E_R_MDM_H5N1)

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Human monocyte-derived macrophages (MDM) serve as a model for resident alveolar macrophages (AM) in the human respiratory tract. MDM cells were analyzed at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.

Host Factor Experiment (CA04M001-P)

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Purpose: To look at the host response to different doses across 4 time points after infection. Samples were collected for both transcriptomics and proteomics. General Design: 20 week-old C57Bl6 mice; Three Doses = 1E3, 1E4, 1E5, 1E6 (PFU); Time points of 1, 2, 4 and 7 days; ~5 mice/time point for infections; 3 mice/timepoint for time matched mocks

Host Factor Experiment (IM015-R)

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Purpose: To obtain lung samples from C57BL6 or RIPK3 knock-out mice infected with A/CA/04/2009 (H1N1) virus for transcriptional analysis. Details: Time Points = 2 and 4 days post-infection; 2-3 replicates for infected and mock mice; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^5 pfu.

Host Factor Experiment (SBRI_LN-L)

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Purpose: To obtain broncho-alveolar lavage from mice infected with influenza A virus, PR8 (280 PFU), or mock for analysis of lipidomic host response: Time Points: 3, 6, 9, 11 d post infection, done in 5 replicates.)

Host Factor Experiment (FLX0001-siSETX)

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The human helicase senataxin (SETX) has been linked to the neurodegenerative diseases amyotrophic lateral sclerosis (ALS4) and ataxia with oculomotor apraxia (AOA2). Here we identified a role for SETX in controlling the antiviral response. Cells that had undergone depletion of SETX and SETX-deficient cells derived from patients with AOA2 had higher expression of antiviral mediators in response to infection than did wild-type cells. Mechanistically, we propose a model whereby SETX attenuates the activity of RNA...

Host Factor Experiment (ICL011-R)

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Purpose: To obtain samples from Calu-3 cells infected with mutants A/Vietnam/1203-CIP048_RG2/2004 (H5N1) and A/Vietnam/1203-CIP048_RG3/2004 (H5N1). Details: Time Points = 0, 3, 7, 12, 18 and 24hrs post infection were taken for mutant viruses while wild type virus was only tested at 7 and 24hrs post-infection; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock...

Host Factor Experiment (ICL004-P)

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Purpose: To obtain samples from Calu-3 cells infected with A/Vietnam/1203/2004(H5N1) for both transcriptional and proteomics analyses. Details: Time Points = 0, 3, 7, 12, 18, and 24 h post infection; Done in triplicate for both RNA and Protein; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of samples; Culture medium (the...

Host Factor Experiment (ICL006-P)

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Purpose: To obtain samples from Calu-3 cells infected with A/CA/04/09 (H1N1) for both transcriptional and proteomics analyses. Details: Time Points = 0, 3, 7, 12, 18, 24, 30, 36 and 48 h post infection; Done in triplicate for both RNA and protein; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of...

Host Factor Experiment (E_R_HTBE_H1N1)

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Human tracheobronchial epithelial (HTBE) cells are considered to serve as a good correlate of influenza virus infection in the human respiratory tract. All HTBE cells used in this study were obtained from a single donor. mRNA-Seq analysis was used to profile the cellular transcriptome of HTBE cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an...

Host Factor Experiment (E_R_HTBE_H5N1)

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Human tracheobronchial epithelial (HTBE) cells are considered to serve as a good correlate of influenza virus infection in the human respiratory tract. All HTBE cells used in this study were obtained from a single donor. mRNA-Seq analysis was used to profile the cellular transcriptome of HTBE cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an...

Host Factor Experiment (IM001-R)

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Purpose: To look at the host response to different doses across 4 time points after infection. Samples were collected for both transcriptomics and proteomics.General Design: 20 week-old C57Bl6 mice; ThreeDoses = 1E2, 1E3, & 1E4 (PFU); Time points of 1, 2, 4 and 7 days; ~5 mice/time point for infections; 3 mice/timepoint for time matched mocks

Host Factor Experiment (IM006B-P)

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Purpose: To obtain lung samples from C57BL6 mice infected with Vietnam/1203-CIP048_RG3/2004 (H5N1) for both transcriptional and proteomic analyses. Details: Time Points = 1, 2, 4 and 7 days post infection; 5 replicates for infected mice and triplicate mice for the mocks; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^4 PFU.

Host Factor Experiment (IM006B-R)

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Purpose: To obtain lung samples from C57BL6 mice infected with Vietnam/1203-CIP048_RG3/2004 (H5N1) for both transcriptional and proteomic analyses. Details: Time Points = 1, 2, 4 and 7 days post infection; 5 replicates for infected mice and triplicate mice for the mocks; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^4 PFU.

Host Factor Experiment (IM006A-R)

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Purpose: To obtain lung samples from C57BL6 mice infected with Vietnam/1203-CIP048_RG3/2004 (H5N1) for both transcriptional and proteomic analyses. Details: Time Points = 1, 2, 4 and 7 days post infection; 5 replicates for infected mice and triplicate mice for the mocks; Inoculation medium for mock infection was the same as the medium used for virus infection. Infection dose was 10^3 PFU.

Host Factor Experiment (ICL006-R)

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Purpose: To obtain samples from Calu-3 cells infected with A/CA/04/09 (H1N1) for both transcriptional and proteomics analyses. Details: Time Points = 0, 3, 7, 12, 18, 24, 30, 36 and 48 h post infection; Done in triplicate for both RNA and protein; Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates; Time matched mocks done in triplicate from same cell stock as rest of...

Host Factor Experiment (IM004-P)

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Purpose: To look at the host response to different doses across 4 time points after infection. Samples were collected for both transcriptomics and proteomics.General Design: 20 week-old C57Bl6 mice; doses = 1E4 PFU; Time points of 1, 2, 4 and 7 days; ~5 mice/time point for infections; 3 mice/timepoint for time matched mocks

Registration Year

  • 2020
    47

Resource Types

  • Dataset
    44
  • Other
    3

Affiliations

  • University of Washington
    30
  • Icahn School of Medicine at Mount Sinai
    12
  • Seattle Institute for Biomedical and Clinical Research
    5