Supplemental data to \"Estimating the Genetic Diversity of Pacific salmon and trout using Multi-gene eDNA Metabarcoding\"

Kevin Weitemier, Brooke E. Penaluna, Laura Hauck, Lucas J. Longway, Tiffany Garcia & Richard Cronn
This dataset contains DNA sequence data of Oncorhynchus species, isolated from environmental DNA (eDNA) from Pacific Northwest streams via microfluidic eDNA metabarcoding and high-throughput (Ilummina) sequencing (samples collected from 2017-09-22 to 2017-10-10). It is accompanied by scripts and commands for data analyses including: sequence denoising, calculation of entropy values by codon position, and calculation of diversity statistics and haplotype mapping. Intermediate outputs include denoised haplotypes, entropy calculations, and haplotype summaries following chimera removal.

Registration Year

  • 2020
    1

Resource Types

  • Dataset
    1

Affiliations

  • Oregon State University
    1
  • United States Department of Agriculture
    1
  • Pacific Northwest Research Station
    1
  • US Forest Service
    1