34 Works

TFC Experiments and Controls (Figure 1b-e, 3e, 5, Extended Data Figure 1d-e, 6, 9, 10)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Spine Movement Experiments (Extended Data Figure 7)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Phosphorylated ERK Immunostaining Experiments (Figure 2, 6a, Extended Data Figure 2)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Individual Anterolateral Cell Photoconversion Experiments (Figure 6)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Figure 5: Synapse formation occurs preferentially in the ventrolateral half of the dorsal pallium in learner, but not nonlearner, fish.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Extended Data Figure 6: Changes in synapse number for zebrafish exposed to control behavioral paradigms after TFC are not significant.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Extended Data Figure 9: Distributions of lost and gained synapses in the pallium following TFC.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Photobleaching Analysis (used in analyses for Figure 5e and Extended Data Figure 10a)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Extended Data Figure 2: Phosphorylated ERK (pERK) staining shows increased anterolateral activity in learners exposed to the CS following training and in naïve fish exposed to the US.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Extended Data Figure 3: PSD-95.FingR-GFP localizes to synapses in living zebrafish larvae.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Extended Data Figure 5: An algorithm for semi-automated identification of synapses from raw SPIM data.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Anterolateral Cell Regional Photoconversion Experiments (Extended Data Figure 11)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Memory Retention Experiments, Control for Extinction (Figure 1h)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Canonical Template (Extended Data Figure 8)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Extended Data 8: Feature based registration onto a template zebrafish brain.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Extended Data Figure 1: Experimental setup and analysis of tail-flick conditioning (TFC), a behavioral paradigm that stimulates associative memory formation in zebrafish larvae.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Extended Data Figure 11: Anterolateral cell population within the 14-16 dpf zebrafish brain projects out of the dorsal pallium compartment ventrally and posteriorly.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Extended Data Figure 10: Intensity of synaptic labeling does not change with TFC.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Figure 3: Total synapse number and distribution of PSD-95.FingR labeling intensity does not change with memory formation.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Figure 4: Rapid synapse turnover in larval zebrafish.

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Redistribution of synapses following memory formation in larval zebrafish

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Microscopy conditions (Figure 3, Extended Data Figure 5)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

TFC Extinction Experiments (Figure 1g, h)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

PSD-95 FingR Colocalization with Dendritic Spines (Extended Data Figure 3)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

TFC Data for Size and Age Dependence Analysis (Extended Data Figure 1f, 4a, b)

William Dempsey, Zhuowei Du, Thai Truong, Karl Czajkowski, Colton Smith, Anna Nadtochiy, Andrey Andreev, Drew Robson, Jennifer Li, Serina Applebaum, Carl Kesselman, Scott Fraser & Don Arnold

Registration Year

  • 2019
    34

Resource Types

  • Dataset
    34