Engineering high-density endothelial cell monolayers on soft substrates.

Adam Feinberg, James F. Schumacher & Anthony B. Brennan
This study demonstrates that a confluent monolayer of endothelial cells (ECs) can be tissue engineered on a soft substrate with a cell density and morphology that approximates in vivo conditions. We achieved formation of a confluent EC monolayer on polydimethylsiloxane (PDMS) elastomer by microcontact printing of fibronectin (FN) in a square lattice array of 3microm diameter circular islands at a 6microm pitch. Uniform coatings of FN or serum proteins on PDMS or on tissue-culture-treated polystyrene...
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