Microsurgery of Cell Membrane with Femtosecond Laser Pulses for Cell Fusion and Optical Injection

Ilina, I.V.; Joint Institute For High Temperatures Of The Russian Academy Of Sciences, Izhorskaya Str., 13-2, Moscow, 125412, Russian Federation, Chefonov, O.V.; Joint Institute For High Temperatures Of The Russian Academy Of Sciences, Izhorskaya Str., 13-2, Moscow, 125412, Russian Federation, Agranat, M.B.; Joint Institute For High Temperatures Of The Russian Academy Of Sciences, Izhorskaya Str., 13-2, Moscow, 125412, Russian Federation, Khramova, Yu.V.; M.V. Lomonosov Moscow State University, Biological Faculty, Leninskie Gory, 1-12, Moscow, 119234, Russian Federation, Ovchinnikov, A.V.; Joint Institute For High Temperatures Of The Russian Academy Of Sciences, Izhorskaya Str., 13-2, Moscow, 125412, Russian Federation, Sitnikov, D.S.; Joint Institute For High Temperatures Of The Russian Academy Of Sciences, Izhorskaya Str., 13-2, Moscow, 125412, Russian Federation & Semenova, M.L.; M.V. Lomonosov Moscow State University, Biological Faculty, Leninskie Gory, 1-12, Moscow, 119234, Russian Federation
We report on results of using femtosecond laserscalpel for microsurgery of plasma membrane ofliving cells. Femtosecond laser pulses were appliedto initiate cell fusion as well as to performreversible permeabilization of cell membranerequired for efficient injection of extrinsicsubstances into the target cells. Laser-based cellfusion of mammalian embryo blastomeres as wellas fusion of cell bodies of neurons of molluskLymnaea stagnalis were successfully carried out byapplying single femtosecond laser pulses (secondharmonic of a Cr:Forsterite laser system) 620 nm,100...