Fluorescent properties of the kindling fluorescent protein (KFP) at acidic pH values

Nemukhin, A.V.; M.V. Lomonosov Moscow State University, Department Of Chemistry, 119991, Moscow, Russia, Goryashchenko, A.S.; A.N. Bach Institute Of Biochemistry, Moscow, 119071, Russia, Mironov, V.A.; M.V. Lomonosov Moscow State University, Department Of Chemistry, 119991, Moscow, Russia, Savitsky, A.P.; A.N. Bach Institute Of Biochemistry, Moscow, 119071, Russia & Rusanov, A.L.; A.N. Bach Institute Of Biochemistry, Moscow, 119071, Russia
Kindling fluorescent protein (KFP) is the photoswitchable protein which can be used in high-resolution microscopy and as a quencher in FRET-sensors. Fluorescent properties of KFP depend on pH value. In this paper we investigate the influence of pH on the spectral properties and kindling/quenching ability of KFP in the acidic pH region.Shift to the acidic region leads to the increase of fluorescence intensity of KFP over time. The excitation spectrum has a new peak near...