Signal Enhancement in Disperse Solutions for the Analysis of Biomedical Samples by Photothermal Spectroscopy

Proskurnin, M.A.; Analytical Chemistry Division, Chemistry Department, Lomonosov Moscow State University, Moscow, 119991, Russia, Nedosekin, D.A.; Philips Classic Laser Laboratories, University Of Arkansas For Medical Sciences, Little Rock, Arkansas, 72205, USA, Ryndina, E.S.; Analytical Chemistry Division, Chemistry Department, Lomonosov Moscow State University, Moscow, 119991, Russia, Zharov, V.P.; Philips Classic Laser Laboratories, University Of Arkansas For Medical Sciences, Little Rock, Arkansas, 72205, USA & Volkov, D.S.; Analytical Chemistry Division, Chemistry Department, Lomonosov Moscow State University, Moscow, 119991, Russia
Photothermal lens spectrometry not only shows high sensitivity of colored heme protein determination, but also provides a change in the sensitivity compared to the theoretical values due to changes in the heat transfer in dispersed media. This can be used for estimating the size of disperse particles exemplified by hemoglobin cyanide, photothermal examination of the state of existence of hemoglobin in highly saline solutions by changes in photothermal properties upon dissociation of hemoglobin tetramers into...