Matrigel plug assay: evaluation of the angiogenic response by reverse transcription-quantitative PCR

Daniela Coltrini, Emanuela Di Salle, Roberto Ronca, Mirella Belleri, Chiara Testini & Marco Presta
The subcutaneous Matrigel plug assay in mice is a method of choice for the in vivo evaluation of pro- and anti-angiogenic molecules. However, quantification of the angiogenic response in the plug remains a problematic task. Here we report a simple, rapid, unbiased and reverse transcription-quantitative PCR (RT-qPCR) method to investigate the angiogenic process occurring in the Matrigel plug in response to fibroblast growth factor-2 (FGF2). To this purpose, a fixed amount of human cells were...
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