Dataset related to the named publication by Palmer et al in Biomedical Optics Express regarding autofluorescence spectroscopy of the endogenous fluorophores collagen, elastin, NADH and flavins in a developing lab based model of muscle invasive urinary bladder cancer. Data from spectroscopy measurements is included in the form of an excel spreadsheet, including day (0-21), sample number, opinion (1 = control, 2 = cancer organoid) and the values of amplitude of fluorescence and diagnostic ratios given...