A novel cyclodextrin glycosyltransferase from alkaliphilic amphibacillus sp. NPST-10 : purification and properties

Abdelnasser S. S. Ibrahim, Ali A. Al-Salamah, Mohamed A. El-Tayeb, Yahya B. Elbadawi & Garabed Antranikian
Screening for cyclodextrin glycosyltransferase (CGTase)-producing alkaliphilic bacteria from samples collected from hyper saline soda lakes (Wadi Natrun Valley, Egypt), resulted in isolation of potent CGTase producing alkaliphilic bacterium, termed NPST-10. 16S rDNA sequence analysis identified the isolate as Amphibacillus sp. CGTase was purified to homogeneity up to 22.1 fold by starch adsorption and anion exchange chromatography with a yield of 44.7%. The purified enzyme was a monomeric protein with an estimated molecular weight of 92...