Characterization of nanoparticle mediated laser transfection by femtosecond laser pulses for applications in molecular medicine

Markus Schomaker, Dag Heinemann, Stefan Kalies, Saskia Willenbrock, Siegfried Wagner, Ingo Nolte, Tammo Ripken, Hugo Murua Escobar, Heiko Meyer & Alexander Heisterkamp
Background: In molecular medicine, the manipulation of cells is prerequisite to evaluate genes as therapeutic targets or to transfect cells to develop cell therapeutic strategies. To achieve these purposes it is essential that given transfection techniques are capable of handling high cell numbers in reasonable time spans. To fulfill this demand, an alternative nanoparticle mediated laser transfection method is presented herein. The fs-laser excitation of cell-adhered gold nanoparticles evokes localized membrane permeabilization and enables an...
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