Oxygen contents, sulfate concentrations, and changes of IPLs under different storage conditions of samples from ODP Leg 204 and IODP Expedition 311, supplement to: Lin, Yu-Shih; Biddle, Jennifer F; Lipp, Julius S; Orcutt, B; Holler, Thomas; Teske, Andreas P; Hinrichs, Kai-Uwe (2010): Effect of storage conditions on archaeal and bacterial communities in subsurface marine sediments. Geomicrobiology Journal, 27, 261-272

Yu-Shih Lin, Jennifer F Biddle, Julius S Lipp, Beth N Orcutt, Thomas Holler, Andreas P Teske & Kai-Uwe Hinrichs
We have studied the effects of slow infiltration of oxygen on microbial communities in refrigerated legacy samples from ocean drilling expeditions. Storage was in heat-sealed, laminated foil bags with a N2 headspace for geomicrobiological studies. Analysis of microbial lipids suggests that Bacteria were barely detectable in situ but increased remarkably during storage. Detailed molecular examination of a methane-rich sediment horizon showed that refrigeration triggered selective growth of ANME-2 archaea and a drastic change in the...
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