Microscopic images and 2D-MALDI - images of the sponge Aplysina aerophoba in response to grazing and video recording of grazing behavior

Yu-Chen Wu, María García-Altares, Marta Ribes, Ute Hentschel & Lucía Pita
We aimed to investigate the cellular and chemical response of the chemically defended sponge Aplysina aerophoba (Phylum Porifera: Class Demospongiae) to grazing by its specialist Tylodina perversa (Phylum Mollusca: Class Opistobranchia). Three treatments were applied: control, grazing, and mechanical damage. Samples were collected 3 hours, 1 day, 3 days, and 6 days after treatment. The behavior of sea slugs after directly contact with sponge specimen was recorded by using a GoPro Hero 4 camera with...
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