Computer-Based Redesign of a β Sandwich Protein Suggests that Extensive Negative Design Is Not Required for De Novo β Sheet Design

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The de novo design of globular β-sheet proteins remains largely an unsolved problem. It is unclear if most designs are failing because the designed sequences do not have favorable energies in the target conformations or if more emphasis should be placed on negative design, i.e. explicitly identifying sequences that have poor energies when adopting undesired conformations. We tested if we could redesign the sequence of a naturally occurring β-sheet protein, tenascin, with a design algorithm...
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