GBM cell culture in PLA under 2D vs 3D for day 4,7,14

Liang Ma
At Day 4, 7, 14, total RNA of GBM cells was isolated with Trizol followed by manufacturer’s instruction (Invitrogen, San Diego, CA). For 2D cell cultures, regular isolation protocol was adopted. For 3D cell cultures, the scaffolds contain cells were cut into small pieces by scissors, then 1mL Trizol was added to the mixture. Total RNA was purified by Qigen RNeasy mini kit (Qiagen, Velencia, CA). Purified RNA was analyzed on a ND-1000 spectrophotometer (Nanodrop...
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