A quantitative map of human Condensins provides new insights into mitotic chromosome architecture

Jan Ellenberg
Single Colour - Condensin subunits were immunostained (with an anti-GFP antibody), and mitotic cells with chromatids oriented in parallel to the focal plane were selected for imaging. DNA (Hoechst) and Condensins (mEGFP tag and anti-GFP immunostaining) were imaged by diffraction-limited microscopy, whereas immunostained Condensin was also imaged by STED microscopy. Double Colour - NCAPH2-mEGFP and NCAPH-Halo were immunostained respectively with anti-GFP (1:500; ab13970; Abcam) + goat secondary anti-chicken Alexa Fluor 594 (1;500; A-11042; Thermo Fisher...
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