SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette

Daniel J. Dickinson, Mark M. Slabodnick, Alicia H. Chen & Bob Goldstein
The advent of CRISPR/Cas9 technology in C. elegans has enabled an unprecedented level of control over this organism’s genome, which is facilitating research in a wide variety of fields. Several different experimental approaches exist for CRISPR in this organism (Dickinson and Goldstein 2016). One particularly powerful approach uses a drug selection with a Self-Excising Cassette (SEC) to isolate the desired genetically-modified animals following Cas9-triggered homologous recombination (Dickinson et al. 2015). The most labor-intensive step in...
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