Pannexin 1 (Panx1) channels are ion channels expressed in a wide range of tissues in vertebrate animals. Panx1 is predicted to be responsible for the release of ATP and other large signaling metabolites from cells. Panx1 has numerous activation mechanisms including via Gq-GPCRs and via C-terminal tail cleavage. The channel is important in this regard for various cell signaling pathways in numerous (patho)physiological processes. Despite works by previous groups, there are still numerous questions regarding...