Genetically Encoded Far-Red Fluorescent Zn2+ and Ca2+ Indicators

Tianchen Wu
The color palette of genetically encoded fluorescent protein indicators has expanded rapidly in recent years. Genetically encoded indicators with excitation and emission within the “optical window” above 600 nm are expected to be superior in many aspects, such as enhanced tissue penetration, reduced autofluorescence and scattering, and lower phototoxicity. We first explored the tolerance of two far-red fluorescent proteins (FPs), mMaroon1 and mCarmine, towards circular permutation. Five circularly permuted far-red FPs (cpFrFPs) with excitation and...
This data repository is not currently reporting usage information. For information on how your repository can submit usage information, please see our documentation.