Ion/ion Reactions in Primary Structure Interrogation of Intact Proteins by Mass Spectrometry

Elizabeth Duselis
This dissertation describes method development for intact protein characterization by mass spectrometry with ion/ion reactions. In this first project, novel methodology of sequential ion/ion reactions, electron transfer dissociation (ETD) followed by ion/ion proton transfer, is used on intact hemoglobin subunits in clinical samples to identify single amino acid substitutions. In this study, 22 variants were definitively identified, including a previously unreported hemoglobin variant now known as Hemoglobin Charlottesville. In the second portion of the dissertation,...
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