Transcriptional Regulation by Mot1 and Spt16

Jason True
The Saccharomyces cerevisiae ATPase Mot1 regulates transcription by impacting the distribution and activity of TATA-binding protein (TBP). In vitro, Mot1 forms a complex with TBP and DNA, and uses ATP hydrolysis to dissociate TBP from DNA. To gain insight into the Mot1 mechanism, we employed a DNA tethered cleavage approach to map regions of Mot1 in proximity to DNA under different conditions. We present evidence for two conformations of the Mot1 ATPase, the detection of...
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