FCS investigations on the diffusional behaviour of TNF-Receptors upon stimulation in living cells

Margarita Gerken
In a cell many biochemical and biophysical processes like signal transduction or the assembly of supramolecular structures are driven by diffusion. To gain insight in these processes it is necessary to obtain information about the diffusion coefficients and the concentrations of the involved molecules. The method of choice to observe diffusion processes and quantifying concentrations is the fluorescence correlation spectroscopy (FCS) which analyses the time-dependent intensity of a fluorescent molecule diffusing through the focus of...
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