Verfahren zur Bestimmung der alkalischen Phosphatase-Aktivität

A. P. Osipov, N. K. Gapanova, A. M. Egorov & I. V. Berezin
The activity of alk. phosphatase is detd. by incubation of a sample with NADP (I) in a buffer medium with subsequent spectrophotometric recording of the hydrolysis product in an enzymic regeneration system of a cofactor-contg. alk. dehydrogenase and two substrates, one of which is EtOH. The sensitivity of the detn. is increased and a rapid and simplified detn. is ensured by incubation of the sample and recording of the hydrolysis product catalyzed by alk. phosphatase...
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