A new turbidimetric method for assaying serum C-reactive protein based on phosphocholine interaction

Pierrot L. Tugirimana, Astrid L. Holderbeke, Jos A. Kint & Joris R. Delanghe
Background: C-reactive protein (CRP) is able to bind phospholipids (mainly phosphocholine) in the presence of calcium ions. We investigated the use of this property for developing an affordable turbidimetrical CRP assay based on diluted soy oil. Methods: Serum (or heparinized plasma) was mixed with Intralipid® 20% in Tris-calcium buffer (pH 7.5). After 30 min of incubation at 37°C, the CRP-phospholipids complexes were measured by turbidimetry (660 nm/700 nm) with a Cobas 6000 analyzer (Roche). Results...
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